Cytotoxic Effect of Caralluma fimbriata Against Human Colon Cancer Cells

Ashwini, S; Ezhilarasan, Devaraj; Anitha, R.

doi:10.5530/pj.2017.2.34

Cited by 57 publications

(44 citation statements)

References 20 publications

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“…Many plant products are useful in diabetes mellitus, anxiety, cancer and many such health problems. [20][21][22][23] Apart from an appraisal of product safety and effectiveness, attention should be paid to the potential that these product ingredients may interact with medications. In recent years, dietary carotenoids, especially xanthophylls, have attracted significant attention because of their activities as antioxidants and their roles in preventing cancer and age-related macular degeneration.…”

Section: Discussionmentioning

confidence: 99%

Effect of Lutein on Cytochrome P450 (Isoform CYP3A4) - An in vitro Study

Meenapriya¹,

Anitha²,

Lakshmi³

2018

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Background: Lutein is a carotenoids vitamin rich in many fruits and vegetables and also available in multivitamin products. It is consumed for its effect on eye disease, cancer, diabetes and other health conditions. Recently, herbal preparations are increasingly used in healthcare systems and concomitant administration of synthetic medications may cause pharmacokinetic or pharmacodynamic interactions leading to very serious medical problems. Understanding the ability of herbal extracts and preparations to modulate the metabolizing enzymes can help the health system for proper treatment of patients and thereby can avoid many adverse effects associated with it. The aim of the study was to find the effect of lutein isolated from Tagetes erecta L on cytochrome P450 isoform CYP3A4. Materials and Methods: The different concentrations of lutein (5-100µg/ml), potassium phosphate buffer, CYP450 reagent and substrate 7-Benzyloxy-4-trifluoromethylcoumarin (BFC) were added to a 96-well plate. The fluorescent intensities of the products were measured by Perkin Elmer Enspire fluorescence reader using an excitation and emission wavelength of 405 nm and 460 nm, respectively to examine the effect of lutein on Cytochrome P 450 isoform CYP3A4 and the IC 50 was calculated by plotting concentrations of lutein against the corresponding percent inhibition. Results: All the tested concentrations of lutein showed potent inhibition against CYP3A4 in a dose-dependent manner. The IC 50 value was found to be 35.27µg/ml. Conclusion: The inhibitory effect of lutein indicates the possibilities of herb-drug interaction if it is co-administered with prescribed drugs that are normally metabolised by CYP3A4 enzyme.

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Section: Discussionmentioning

confidence: 99%

Effect of Lutein on Cytochrome P450 (Isoform CYP3A4) - An in vitro Study

Meenapriya¹,

Anitha²,

Lakshmi³

2018

Self Cite

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“…Apart from intake of a product and looking into its safety and effectiveness, attention should be paid to the potential that these product ingredients may interact with medications [17,18] as many plant products are useful in treating various disease conditions such as diabetes mellitus, anxiety, cancer, and many other health problems [19][20][21][22]. Hence, proper care should be taken to administer such products after confirming its effect on various metabolizing enzymes.…”

Section: Discussionmentioning

confidence: 99%

Tamarind Extract Inhibits Cytochrome P450 (Cyp3a4 Isozyme) - An in Vitro Study

Anitha

Thangavelu

2018

Asian J Pharm Clin Res

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Objective: The aim of the present study was to analyze the effect of the aqueous fruit pulp extract of Tamarindus indica L. (tamarind extract) on cytochrome P 450 isoform CYP3A4.Methods: Tamarind extract at different concentrations from 5 to 100 μg/ml was examined for its inhibitory property toward cytochrome P 450 isoform CYP3A4. The various concentrations of tamarind extract, potassium phosphate buffer, CYP450 reagent, and substrate 7-Benzyloxy-4- trifluoromethylcoumarin were added to a 96-well plate. The mixtures were preincubated for 20 min at room temperature. The reaction was started by a mixture of free constituted substrate and NADP+ and incubated at room temperature for 30–60 min. The reaction was stopped by Tris-HCl buffer, pH 10.5. The fluorescent intensities of the products were measured by PerkinElmer Enspire fluorescence reader using an excitation and emission wavelength of 405 nm and 460 nm, respectively. Inhibitory concentration (IC50) was calculated by plotting concentrations of tamarind extract against the corresponding percentage inhibition.Results: All the tested concentrations of extract except 5 μg/ml showed good inhibition against CYP3A4 in a dose-dependent manner. The IC50 value of tamarind for CYP3A4 inhibitory activity was found to be 27.89 μg/ml.Conclusion: T. indica aqueous fruit pulp extract exhibited an inhibitory effect on CYP34A, thereby indicating the possibilities of herb-drug interaction if these extracts are coadministered with the prescribed drugs that are metabolized by CYP3A4.

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“…Desired concentrations of test compounds were prepared in 0.1% di-methyl sulfoxide (DMSO) prior to the experiment. 17 The reactant mixtures were diluted with media and cells were treated with different concentration ranges of the extract (3.125-200 μg/mL) and incubated for 72 h respectively which was the optimal treatment time of the extracts in each of the cell lines. The effect induced was also compared to the standard drug used viz.…”

Section: Treatment Groupsmentioning

confidence: 99%

Evaluation of in vitro Antioxidant and Anticancer Activity of Simarouba glauca Leaf Extracts on T-24 Bladder Cancer Cell Line

Puranik¹,

Ghagane²,

Nerli³

et al. 2017

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Objective: Screening of preliminary phytochemicals, evaluation of in vitro antioxidant and in vitro anticancer activities of Simarouba glauca leaf extracts on T-24 Bladder cancer cell line. Materials and Methods: Herbal extraction was carried out by Soxhlet method using chloroform, ethylacetate, methanol, ethanol, aqueous and hydroalcohol. Phytochemical investigation was done using biochemical tests. Total phenolic content was estimated by Folin-Ciocalteu reagent (FCR) method. Antioxidant potential of leaf extracts was analyzed by Ferric ion reducing antioxidant power (FRAP) assay, Phosphomolybdenum (PM) assay and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. In vitro anticancer activity on T-24 bladder cancer cell line was assessed by MTT assay. Statistical analysis used: Statistical analysis of data was performed by analysis of variance (one-way ANOVA) and level of statistical significance between groups was carried out using GraphPad Prism version 5.0 for Windows (GraphPad Software, San Diego, CA, USA). Results: Phytochemical analysis revealed the presence of rich secondary metabolite present in all the solvent extracts. Hydroalcoholic extract showed highest presence of phenolic content (92.38±0.29 mg/g) GAE. Ethanol and methanol extract showed highest antioxidant capacity in DPPH, FRAP and PM assay as compared to the other extracts based on the test performed. The results confirmed that ethanol extract significantly (p<0.05) inhibited T-24 cell line with IC 50 value (533.55±25.02 µg/mL) as compared to standard drug doxorubicin (0.16µM/mL). Conclusions: The results of the present findings strengthen the potential property of Simarouba glauca as a resource for the discovery of novel antioxidant and anticancer agents.Key words: Antioxidant, Anticancer, Bladder Cancer, Phytochemical, Simarouba glauca. Key Messages: The use of medicinal plants have been practiced to treat cancer aliments across the world. Our study demonstrates the implication of herbal extracts to inhibit cancer cell lines at in vitro levels. However, the further approaches at in vivo level using lead compounds will give core insights of S. glauca extracts on Bladder cancer.

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Cytotoxic Effect of Caralluma fimbriata Against Human Colon Cancer Cells

Cited by 57 publications

References 20 publications

Effect of Lutein on Cytochrome P450 (Isoform CYP3A4) - An in vitro Study

Effect of Lutein on Cytochrome P450 (Isoform CYP3A4) - An in vitro Study

Tamarind Extract Inhibits Cytochrome P450 (Cyp3a4 Isozyme) - An in Vitro Study

Evaluation of in vitro Antioxidant and Anticancer Activity of Simarouba glauca Leaf Extracts on T-24 Bladder Cancer Cell Line

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