Cytotoxic effect of inositol hexaphosphate and its Ni(II) complex on human acute leukemia Jurkat T cells

Abstract

Inositol hexaphosphate (InsP6) is present in cereals, legumes, nuts and seed oils and is biologically active against some tumor and cancer cells. Herein, this study aimed at evaluating the cellular toxicity, antiproliferative activity and effects on cell cycle progression of free InsP6 and InsP6-Ni(II) of leukemic T (Jurkat) and normal human cells. Treatments with InsP6 at concentrations between 1.0 and 4.0mM significantly decreased the viability of Jurkat cells, but showed no cytotoxic effect on normal human lymphocytes. Treatment with InsP6-Ni(II) complex at concentrations between 0.05 and 0.30 mM showed an anti-proliferative dose and a time-dependent effect, with significantly reduced cell viability of Jurkat cells but showed no cytotoxic effect on normal human lymphocytes as compared to the control. Ni(II) free ion was toxic to normal cells while InsP6-Ni(II) had no cytotoxic effect. The InsP6-Ni(II) complex potentiated (up to 10×) the antiproliferative effect of free InsP6 on Jurkat cells. The cytometric flow assay showed that InsP6 led to an accumulation of cells in the G0/G1 phase of the cell cycle, accompanied by a decrease in the number of cells in S and G2/M phases, whereas InsP6-Ni(II) has led to an accumulation of cells in the S and G2/M phases. Our findings showed that InsP6-Ni(II) potentiates cytotoxic effects of InsP6 on Jurkat cells and may be a potential adjuvant in the treatment of cancer.

Glycerylphytate compounds with tunable ion affinity and osteogenic properties

“…49 reported that PA extracted from rice bran was not toxic up to 6 mM for 72 h in untransformed cultures of 3T3 cells, yielding < 10% of dead cells 49 , Similar results were reported by de Lima et al . 50 who did not observe PA cytotoxicity at 4.0 mM for 48 h in lymphocytes isolated from healthy human donors. In this work, cytotoxicity (IC 50 ) of PA and derivatives was tested in MSCs cultures using a standardized Alamar Blue assay.…”

“…Biological properties of PA have been widely studied in different in vitro and in vivo models 2,47–50 , Using healthy cells, Norhaizan et al . 49 reported that PA extracted from rice bran was not toxic up to 6 mM for 72 h in untransformed cultures of 3T3 cells, yielding < 10% of dead cells 49 , Similar results were reported by de Lima et al .…”

Physicochemical characterization of Theobroma cacao L. mucilage, in Ecuadorian coast

“…This compound has been employed in growth inhibition assays of human tumor cell lines (De Lima et al, 2015), as a protective agent in fishes with copper exposure (Jiang et al, 2014), as indicator during cellular apoptosis (Agarwal et al, 2010). Other compound: Maltose (0.02%) is a disaccharide that it has too many applications.…”

Combined toxicity and underlying mechanisms of a mixture of eight heavy metals

“…Cadmium can induce a delay in the transition between the G1 and S phases of the cell cycle, and slows progression through the S phase (28). Nickel (II) modulates cellular responses through effectors involved in G2/M arrest and apoptosis regulatory pathways (32,33). Rodríguez-Sastre et al (34) evaluated the capability of a mixture of 2 µM NaAsO 2 , 2 µM CdCl 2 and 5 µM Pb(C 2 H 3 O 2 ) 2 •3H 2 O at relevant epidemiological concentrations to induce cell transformation processes.…”