Cytotoxic Effect of the Paku Atai Merah (Angiopteris ferox Copel) Fraction on MCF-7 and HeLa Cells and its Compound Profile by GC-MS

Nur, Syamsu; Aisyah, Andi Nur; Lukitaningsih, Endang; Rumiyati, Rumiyati; Fadri, Alfat; Marwati, Marwati

doi:10.33084/bjop.v5i1.2943

Cited by 3 publications

(2 citation statements)

References 23 publications

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“…MTT measures cell respiration and the amount of formazan formed is proportional to the number of living cells present in culture. An increase or decrease in the number of cells results in a simultaneous change in the amount of formazan formed, the effect of the level of cytotoxicity caused by the substance (Nur et al, 2021;2022).…”

Section: Discussionmentioning

confidence: 99%

Isolation, identification and biochemical studies of gallic acid from Turbinaria decurrens Bory

Sami¹,

Nur²

2022

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Cancer is one of the diseases that cause the biggest death in the world. The research for natural compounds derived from marine is very developed at this time, especially in algae. Brown algae have more phenolic content than other types of algae, T. decurrens Bory is one of the brown algae that had taken from the Indonesian island of Dutungan, South Sulawesi. Phenolic groups are also known to be able to inhibit the growth of cancer cells. The purpose of this study is to isolate and identify compounds using spectrophotometer instruments. The isolates obtained were tested for anticancer activity against H460 and MCF-7 cells. Extraction was carried out by maceration method, fractionation was done by chromatography column eluent n-hexane:EtOAc: MeOH by increasing polarity. The structure determination is done based on the interpretation of FT-IR, 1D-NMR 1H,13C, and ESI-LCMS spectra data and anticancer activity test used MTT bioassay. The isolate is known as gallic acid, and the isolate was tested for anticancer activity against H460 and MCF-7 cells. The result is obtained IC50 value for H460 cells 5.69 μg/ml and MCF-7 cells 4.63 μg/ml. As a positive control used cisplatin with an IC50 value of 5.81 μg/ml against H460 cells and MCF-7 cells with a value of 5.59 μg/ml. Gallic acid has a higher toxic effect compared to cisplatin. One phenolic compound has been found in T. decurrens Bory which is gallic acid. Gallic acid has a higher toxic effect than cisplatin, this compound can be used as an anticancer agent.

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Section: Discussionmentioning

confidence: 99%

Isolation, identification and biochemical studies of gallic acid from Turbinaria decurrens Bory

Sami¹,

Nur²

2022

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“…Bahan yang digunakan ialah aerosil, aquadest, ekstrak etanol umbi paku atai merah, laktosa, magnesium stearat, n-heksan, natrium karboksilmetil selulosa (Na CMC), parafin cair, polivinil pirolidon (PVP), starch 1500, dan talkum. 12,13 .…”

Section: Bahanunclassified

Formulation and Test of Physical Characteristics of Ethanol Extract Capsules of Red Atai Tuber (Angiopteris ferox Copel)

Aisyah,

Khairi,

Nur

et al. 2023

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Umbi paku atai merah (Angiopteris ferox Copel) merupakan tumbuhan paku yang berasal dari suku Dayak yang digunakan sebagai obat tradisional yang berpotensi sebagai antioksidan karena memiliki kandungan senyawa metabolit sekunder meliputii alkaloid, steroid, flavanoid, tanin serta saponin. Penggunaan umbi paku atai merah di masyarakat masih menggunakan cara tradisional yaitu diseduh dengan air panas, diminum air rebusannya dan meninggalkan rasa yang tidak enak, sehingga tujuan dari penelitian ini yaitu untuk memformulasi sediaan kapsul ekstrak etanol umbi paku atai merah yang memenuhi karakteristik fisik. Penelitian ini dilakukan pembuatan sediaan kapsul yang berisi granul dengan variasi konsentrasi aerosil FI 5%, FII 7% dan FIII 9%. Granul dibuat dengan metode granulasi basah, lalu dilakukan evaluasi karakteristik fisik granul dan kapsul. Hasil yang diperoleh dari ketiga granul tersebut memenuhi karakteristik fisik granul dengan parameter uji organoleptik, kandungan lembab, susut pengeringan, kecepatan alir, sudut istirahat, Bj sejati, Bj nyata, Bj mampat dan porositas. Sedangkan untuk kapsul FI dan FII merupakan sediaan yang memenuhi karakteristik fisik kapsul dengan parameter uji keseragaman bobot, waktu hancur dan higroskopis.

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Cytotoxic Effect of the Paku Atai Merah (Angiopteris ferox Copel) Fraction on MCF-7 and HeLa Cells and its Compound Profile by GC-MS

et al. 2022

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Cancer is a condition of abnormal cell proliferation of tissue cells in the body that becomes malignant. It can attack other parts of the body and affect the normal function of the body organs. The sample used in this study was tubers of paku atai merah (Angiopteris ferox Copel), then extracted using 96% ethanol eluent to obtain a thick extract. The ethanolic extract of A. ferox was fractionated using column chromatography to get the active fraction to characterize the compound using thin-layer chromatography and gas chromatography-mass spectroscopy (GC-MS) and tested its cytotoxic effectiveness on MCF-7 and HeLa cancer cells. The results of this study were obtained from fractionation using the column chromatography method to get sub-fraction C and the results of compound characterization using GC-MS and obtained variations in the class of compounds contained in the sample: amino acids, glucosinolates, alkaloids, flavonoids, and terpenoids. Based on the cytotoxic effect test of sub-fraction C on MCF-7 cells, the results obtained moderate cytotoxic effects with an IC50 value of 61.027 µg/mL, and HeLa cells had an IC50 value of 521.03 µg/mL, which was categorized as having a weak cytotoxic effect. Based on the results obtained from this study, it can be concluded that sub-fraction C of A. ferox tubers has a cytotoxic effect on MCF-7 cells to be used as a reference for tracing pure compounds from A. ferox tuber.

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Cytotoxic Effect of the Paku Atai Merah (Angiopteris ferox Copel) Fraction on MCF-7 and HeLa Cells and its Compound Profile by GC-MS

Cited by 3 publications

References 23 publications

Isolation, identification and biochemical studies of gallic acid from Turbinaria decurrens Bory

Isolation, identification and biochemical studies of gallic acid from Turbinaria decurrens Bory

Formulation and Test of Physical Characteristics of Ethanol Extract Capsules of Red Atai Tuber (Angiopteris ferox Copel)

Cytotoxic Effect of the Paku Atai Merah (Angiopteris ferox Copel) Fraction on MCF-7 and HeLa Cells and its Compound Profile by GC-MS

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