Cytotoxic effects of Benzodioxane, Naphthalene diimide, Porphyrin and Acetamol derivatives on HeLa cells

Abstract

Objectives:To synthesize novel compounds belonging to Benzodioxane, Naphthalene diimide, Aminophenol derivatives and Porphyrin classes and test their potential anticancer properties.Methods:Several compounds were synthesized and their molecular identity was confirmed using nuclear magnetic resonance. Potential anticancer properties were determined using cytopathogenicity assays and growth inhibition assays using cervical cancer cells (HeLa). Cells were incubated with different concentrations of compounds belonging to Benzodioxane, Naphthalene diimide, Aminophenol derivatives and Porphyrins and effects were determined. HeLa cells cytopathogenicity was determined by measuring lactate dehydrogenase release using cytotoxicity detection assay. Growth inhibition assays were performed by incubating 50% semi-confluent HeLa cells with Benzodioxane, Naphthalene diimide, Aminophenol derivatives and Porphyrin compounds and HeLa cell proliferation was observed. Growth inhibition and host cell death were compared in the presence and absence of drugs.Results:Cytopathogenicity assays showed that the selected compounds were cytotoxic against HeLa cells, killing up to 90% of cells. Growth inhibition assays exhibited 100% growth inhibition. These effects are likely via oxidative stress, production of reactive oxygen species, changes in cytosolic and intracellular calcium/adenine nucleotide homeostasis, inhibition of ribonucleotide reductase/cyclooxygenase and/or glutathione depletion.Conclusions:Benzodioxane, Naphthalene diimide, Aminophenol derivatives and Porphyrins exhibited potent anticancer properties. These findings are promising and should pave the way in the rationale development of anticancer drugs. Using different cancer cell lines, future studies will determine their potential as anti-tumour agents as well as their precise molecular mode of action.

Antimicrobial activities of green synthesized gums-stabilized nanoparticles loaded with flavonoids

“…HeLa cells were cultured in Roswell Park Memorial Institute (RPMI)−1640 supplemented with 10% fetal bovine serum (FBS), 10% Nu-serum, 2 mM glutamine, 1 mM pyruvate, penicillin and streptomycin (100 units/mL and 100 μg/mL respectively), non-essential amino acids, and vitamins to obtain uniform monolayers of cells in 75-cm 2 culture flasks as described previously 28 . Old media was aspirated, and cells were trypsinized with 2 mL trypsin.…”

“…To evaluate the cytotoxic effects of these nanoparticles on human cell, LDH cytotoxicity assay was performed as reported previously 28 . Briefly, 100 µg per mL concentrations of HDN and NRG loaded nanoparticles and respective controls were treated with uniform monolayer of HeLa cells in a 24-well plate, and the cells were incubated for 24 h at 37 °C in a 5% CO 2 incubator.…”

Cobalt nanoparticles as novel nanotherapeutics against Acanthamoeba castellanii

“…Human keratinocyte cells were cultured in RPMI-1640 supplemented with 10% fetal bovine serum (FBS), 1% (2 mM) l -glutamine, 1% penicillin-streptomycin and 1% nonessential amino acid (NEAA) as previously described [32]. The cells were incubated at 37 °C in a 5% CO 2 incubator with 95% humidity for the formation of uniform monolayers.…”

“…Cytotoxicity of CoNPs towards human keratinocytes cells was tested by treating 100 µg/ml nanoparticles with HaCaT monolayers in 96-well plates by using a lactate dehydrogenase (LDH) assay as described previously [32]. The cells were incubated in a 37 °C, 5% CO 2 incubator for 24 h. Untreated cells in RPMI-1640 only were taken as negative controls and 1% Triton X-100 was used as a positive control.…”

Antiamoebic Properties of Laboratory and Clinically Used Drugs against Naegleria fowleri and Balamuthia mandrillaris

“…Human cervical adenocarcinoma (HeLa) cells (ATCC ® CCL-2 ™ ) were purchased from American Type Culture Collection, while human keratinized skin (HaCaT) cells (CLS:300493) were acquired from CLS Cell Lines. Cells were cultivated in Roswell Park Memorial Institute (RPMI) 1640 medium, supplemented with 10% foetal bovine serum (FBS), 1% L-glutamine, 1% antibiotics and 1% minimum essential medium amino acids at 37 °C and 5% CO 2 in a humidified incubator as previously described [ 23 ].…”