Cytotoxic Effects of Eleutherococcus senticosus Aqueous Extracts in Combination with N6(Δ2-Isopentenyl)-adenosine and 1-β-D-Arabinofuranosylcytosine Against L1210 Leukemia Cells

Hacker, Bruce; Medon, Philip J.

doi:10.1002/jps.2600730235

Cited by 18 publications

(5 citation statements)

References 20 publications

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“…The dose of 1 mg/ml WEE, however, led only to a partial inhibition of proliferation, whereas lower concentrations were free of any antiproliferative effects on normal human lymphocytes. It is surprising that the antiproliferative concentrations for normal fully differentiated human lymphocytes are somewhat higher than those reported for L1210 murine leukemia cells with an ED 50 of approx-imately 75 µg/ml [10]. These functional reductions seem to represent a dose-dependent functional inhibition restricted to the high concentrations.…”

Section: Discussionmentioning

confidence: 84%

Immunopharmacological in vitro Effects of Eleutherococcus senticosus Extracts

Steinmann

Esperester

Joller

2011

Arzneimittelforschung

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The objective of these investigations was to further elucidate the immunopharmacological profile of fluid extracts of Eleutherococcus senticosus and to identify the specific role of its characteristic eleutherosides B and E. An ethanolic dry extract of Eleutherococcus senticosus was used as starting material for the isolation of the eleutherosides B and E. Immunopharmacological studies included expression of major histocompatibility complex class I and II molecules by rat bone marrow-derived mononuclear phagocytes, human lymphocyte marker flow cytometry, and in vitro testing of human lymphocyte functions. In contrast to the isolated eleutherosides B and eleutherosides E and the re-mixed eleutherosides B and E, the whole ethanolic fluid extract of Eleutherococcus senticosus was able to induce and enhance interleukin-1 and interleukin-6 but not interleukin-2 production in vitro. The effective concentration of the whole ethanolic extract ranged from 1.0-0.1 mg/ml for the enhancement of interleuking-1 alpha production and 1.0-0.03 mg/ml for the enhancement of interleukin-6 production. It is concluded that the observed enhancing immunopharmacological activities on acute phase response mediators are best exhibited by the induction with whole ethanolic extracts whereas the species-specific and characteristic eleutherosides B and E are not associated with these activities.

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Section: Discussionmentioning

confidence: 84%

Immunopharmacological in vitro Effects of Eleutherococcus senticosus Extracts

Steinmann

Esperester

Joller

2011

Arzneimittelforschung

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“…The earlier studies performed by Hacker and Medon revealed cytotoxic effect of E. senticosus water extract against L1210 leukemia cells with ED50 value of 75 µg/mL [4]. Other studies performed by Yoon et al [15] have shown anti-metastatic activity of a water extract of E. senticosus.…”

Section: Discussionmentioning

confidence: 91%

“…In turn, the performed studies with the use of other cell lines, such [2,19,6]. Other studies showed that the water extract from the stem bark of A. senticosus induced apoptosis in human stomach cancer KATO III cells [4]. Unfortunately, there is still little information on the antileukemic activity of the other investigated species growing in Asia.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic activity of ethanolic extracts of Eleutherococcus species cultivated in Poland on HL60 leukemia cell line

Załuski

Smolarz

Bogucka‐Kocka

2014

Current Issues in Pharmacy and Medical Sciences

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The Eleutherococcus species including 40 species native to Asia are medicinal plants widely used in traditional medicine. Some of these species are cultivated at the botanical gardens in Europe. On the basis on our earlier studies it was concluded that the extracts of analyzed species act as antioxidants, inhibitors of MMPs, and cytotoxic against Jurkat 45 leukemia cell line. In this study, the anti-leukemic potential of roots and leaves from six Eleutherococcus species cultivated in Poland was determined. The in vitro cytotoxic activity towards human promyelotic leukemia cell line HL60 using trypan blue assay was evaluated. The induction of apoptosis in stimulated leukemia cells was determined by AnnexinV method. Morphological changes in treated cells were observed by microscopic investigations. The results showed that ethanolic extracts from the roots and the leaves of E. senticosus, E. setchuensis, E. sessiliflorus, E.gracilistylus, E. henryi and E. divaricatus exhibit cytotoxic effect towards leukemic HL60 cells. The received IC50 values for roots ranged from 49-208 µg/mL and for the leaves from 116-518 µg/mL. The ethanol extract from the roots of E. divaricatus showed the highest cytotoxic and proapoptotic effect on HL60 human lymphoid leukemia cell line.

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“…Proteins, and other isolates from the cortex of A. senticosus a related genus to E. senticosus to have been shown to confer an anti-tumor effect and increase the survival time in mice (Shan et al, 2004), which may include eleutherosides. The anti-cancer effect of various Siberian ginseng extracts both E. senticosus and A. senticosus have been investigated in a number of studies such as using an aqueous extract of E. senticosus and combining it with either cytarabine or N6-(delta 2-isopentenyl)-adenosine potentiated the anti-proliferative effects against L1210 murine leukemia at 75 µg/mL (ED50) (Hacker et al, 1984). Other than eleutherosides, there are a number of other compounds in E. senticosus and the related species A. senticosus that confer anti-cancer effect.…”

Section: Discussionmentioning

confidence: 99%

Proliferative and Inhibitory Activity of Siberian ginseng (Eleutherococcus senticosus) Extract on Cancer Cell Lines; A-549, XWLC-05, HCT-116, CNE and Beas-2b

Cichello

Yao²,

Dowell³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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Siberian ginseng (Eleutherococcus senticosus) is used primarily as an adaptogen herb and also for its immune stimulant properties in Western herbal medicine. Another closely related species used in East Asian medicine systems i.e. Kampo, TCM (Manchuria, Korea, Japan and Ainu of Hokkaido) and also called Siberian ginseng (Acanthopanax senticosus) also displays immune-stimulant and anti-cancer properties. These may affect tumour growth and also provide an anti-fatigue effect for cancer patients, in particular for those suffering from lung cancer. There is some evidence that a carbohydrate in Siberian ginseng may possess not only immune stimulatory but also anti-tumour effects and also display other various anti-cancer properties. Our study aimed to determine the inhibitory and also proliferative effects of a methanol plant extract of Siberan ginseng (E. senticosus) on various cancer and normal cell lines including: A-549 (small cell lung cancer), XWLC-05 (Yunnan lung cancer cell line), CNE (human nasopharyngeal carcinoma cell line), HCT-116 (human colon cancer) and Beas-2b (human lung epithelial). These cell lines were treated with an extract from E. senticosus that was evaporated and reconstituted in DMSO. Treatment of A-549 (small cell lung cancer) cells with E. senticosus methanolic extract showed a concentration-dependent inhibitory trend from 12.5 -50µg/mL, and then a plateau, whereas at 12.5 and 25 µg/mL, there is a slight growth suppression in QBC-939 cells, but then a steady suppression from 50, 100 and 200µg/mL. Further, in XWLC-05 (Yunnan lung cancer cell line), E. senticosus methanolic extract displayed an inhibitory effect which plateaued with increasing dosage. Next, in CNE (human nasopharyngeal carcinoma cell line) there was a dose dependent proliferative response, whereas in Beas-2 (human lung epithelial cell line), an inhibitory effect. Finally in colon cancer cell line (HCT-116) we observed an initially weak inhibitory effect and then plateau.

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Cytotoxic Effects of Eleutherococcus senticosus Aqueous Extracts in Combination with N6(Δ2-Isopentenyl)-adenosine and 1-β-D-Arabinofuranosylcytosine Against L1210 Leukemia Cells

Cited by 18 publications

References 20 publications

Immunopharmacological in vitro Effects of Eleutherococcus senticosus Extracts

Immunopharmacological in vitro Effects of Eleutherococcus senticosus Extracts

Cytotoxic activity of ethanolic extracts of Eleutherococcus species cultivated in Poland on HL60 leukemia cell line

Proliferative and Inhibitory Activity of Siberian ginseng (Eleutherococcus senticosus) Extract on Cancer Cell Lines; A-549, XWLC-05, HCT-116, CNE and Beas-2b

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