Cytotoxic effects of hard-setting cements applied on the odontoblast cell line MDPC-23

Mendonça, Adriano Augusto Melo de; Souza, Pedro Paulo Chaves de; Hebling, Josimeri; Costa, Carlos Alberto de Souza

doi:10.1016/j.tripleo.2007.05.017

Cited by 24 publications

(18 citation statements)

References 36 publications

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“…In accordance with our results, previous studies 2,5,25) reported that RelyX U200 presented cytotoxic effects in different cell lines, such as odontoblast, fibroblast, and pulp cells. RelyX U200 contains a multifunctional phosphoric-acid-modified methacrylate.…”

Section: Discussionsupporting

confidence: 93%

“…In agreement with our investigation, Bakopoulou et al 26) demonstrated that eluates of RelyX U200 were severely cytotoxic, as the first serial dilution of RelyX U200 caused complete mitotic arrest in lymphocyte cultures. Cementation of inlays using RelyX U200 caused mild inflammatory pulp response in an in vivo study 25) . Maxcem Elite contains methacrylate ester adhesive monomers.…”

Section: Discussionmentioning

confidence: 97%

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Cytotoxicity evaluation of luting resin cements on bovine dental pulp-derived cells (bDPCs) by real-time cell analysis

et al. 2015

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To evaluate the cytotoxicity of resin cements on dental pulp-derived cells (bDPCs), Bifix QM (BQM), Choice 2(C2), RelyX U200(RU200), Maxcem Elite(ME), and Multilink Automix(MA) were tested. The materials were incubated in DMEM for 72 h. A real-time cell analyzer was used to evaluate cell survival. The statistical analyses used were one-way ANOVA and Tukey-Kramer tests. BQM, RU200, and ME demonstrated a significant decrease in the bDPCs' index at 24 and 72 h (p≤0.001). These materials were found to be the most toxic resin cements, as compared to the control and other tested materials (C2 and MA). However, C2 and MA showed a better survival rate, compared to BQM, RU200, and ME, and had lower cell index than the control group. The cytotoxic effects of resin cements on pulpa should be evaluated during the selection of proper cements.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 97%

Cytotoxicity evaluation of luting resin cements on bovine dental pulp-derived cells (bDPCs) by real-time cell analysis

et al. 2015

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“…Generally, information about the toxicity of self-adhesive resin cements is obtained by comparison with other resin cements [27][28][29][30][31] . However, the effects of monomer release by self-adhesive resins on cellular functions, such as cell viability and morphology, ROS generation, and DNA damage, have not been well studied.…”

Section: Toxicity Analysismentioning

confidence: 99%

“…For example, the in vitro toxic effects of TEGDMA and UDMA were studied in L-929 mouse cells instead of human cells. The use of human cells would have been a better choice for simulation; nevertheless, several studies have confirmed that various concentrations of TEGDMA and UDMA provoke cytotoxicity and genotoxicity in human cells 30,33,38) and that both L-929 mouse cells and human cells show comparable responses 41,42) . Another limitation is that self-adhesive cements may contain toxic monomers other than TEGDMA and UDMA that could also be released.…”

Section: Genotoxicity Assaymentioning

confidence: 99%

Evaluation of residual monomer release and toxicity of self-adhesive resin cements

Kurt

Altıntaş

Kızıltaş

et al. 2018

Dental Materials Journal

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The aim of this study was to evaluate the amount of leached residual monomers from self-adhesive resin cements and evaluate their toxicity in-vitro. A total of 60 disk-shaped specimens (5 mm in diameter and 0.5 mm in thickness) were prepared from each cement (RelyX U200, SpeedCEM, G-Cem) (n=20). Specimens were immersed in artificial saliva and the amount of released monomers [urethane dimethacrylate (UDMA) and triethyleneglycol dimethacrylate (TEGDMA)] was identified. Then, the cytotoxicity and genotoxicity effect on cells were evaluated using the defined amounts of released monomers from cements. The highest monomer release was detected in G-Cem (p<0.05). The highest cytotoxicity value was identified from SpeedCEM (p<0.01) and the highest genotoxicity values were calculated from RelyX U200 (p<0.05). Released UDMA and TEGDMA from self-adhesive resin cements induced cytotoxicity and genotoxicity effect on cells.

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“…For this purpose, sterilized 12-mm-diameter cover glasses (Fisher Scientific) were placed at the bottom of the wells of sterile 24-well plates immediately before seeding of MDPC-23 cells. 31 After the incubation period, the extracts that were in contact with the cells were aspirated, and the MDPC-23 cells that remained adhered to the glass substrate (GS) were fixed in 1 mL of 2.5% buffered glutaraldehyde for one hour. These cells were subsequently rinsed three times with 1 mL PBS (five minutes per rinse), postfixed in 1% osmium tetroxide for 60 minutes, and processed for examination with SEM (JEOL-JMS-T33A Scanning Microscope, Peabody, MA, USA).…”

Section: Treatmentsmentioning

confidence: 99%

Effect of Different Light Sources and Enamel Preconditioning on Color Change, H2O2 Penetration, and Cytotoxicity in Bleached Teeth

Gonçalves¹,

Costa

Soares

et al. 2016

Operative Dentistry

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This study evaluated the effects of acid etching of the enamel and the combination of different light sources (halogen light, light-emitting diodes [LEDs], and LED/Laser) and the bleaching product on color change, penetration of hydrogen peroxide (H2O2), and cytotoxicity over time. The color change (ΔE) and the amount of H2O2 that permeated the tooth tissue were analyzed using a spectrophotometer. Cell metabolism and morphology were evaluated using the methylthiazol tetrazolium assay and scanning electron microscopy, respectively. The ΔE values and H2O2 permeation were not significantly different under any of the experimental conditions. Tooth whitening significantly reduced cell metabolism, regardless of whether a light source was used. Preconditioning the enamel did not influence the cellular metabolism in any group. In conclusion, combining the bleaching product with different light sources and/or preconditioning the enamel resulted in few significant changes in color, transenamel and transdentinal penetration of H2O2, or cytotoxicity and cell morphology.

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Cytotoxic effects of hard-setting cements applied on the odontoblast cell line MDPC-23

Cited by 24 publications

References 36 publications

Cytotoxicity evaluation of luting resin cements on bovine dental pulp-derived cells (bDPCs) by real-time cell analysis

Cytotoxicity evaluation of luting resin cements on bovine dental pulp-derived cells (bDPCs) by real-time cell analysis

Evaluation of residual monomer release and toxicity of self-adhesive resin cements

Effect of Different Light Sources and Enamel Preconditioning on Color Change, H2O2 Penetration, and Cytotoxicity in Bleached Teeth

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