Cytotoxic effects of polychlorinated biphenyl hydroquinone metabolites in rat hepatocytes

Chan, Katie; Lehmler, Hans‐Joachim; Sivagnanam, Milani; Feng, Cynthia; Robertson, Larry W.; O’Brien, Peter J.

doi:10.1002/jat.1483

Cited by 13 publications

(13 citation statements)

References 28 publications

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“…The results of this study showed that PCBs exposure caused a significant decrease in the testicular T‐SHs whereas it increased the ROS and TBARS levels, thus evidencing a state of oxidative stress. The reduction in the intracellular T‐SHs level in PCBs‐treated rats could indicate its overutilisation and/or oxidation following PCBs exposure (Chan et al ., ; Aly, ). Pro‐oxidative alterations in the intracellular thiol status are well known to adversely affect cellular processes (Fourquet et al ., ; Kumar et al ., ; Toledano et al ., ; Adedara & Farombi, ).…”

Section: Discussionmentioning

confidence: 97%

Quercetin ameliorates polychlorinated biphenyls-induced testicular DNA damage in rats

et al. 2015

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Polychlorinated biphenyls (PCBs) are a group of environmental contaminants widely reported to cause gonadal toxicity in both humans and animals. This study investigated the amelioratory role of quercetin in PCBs-induced DNA damage in male Wistar rats. Polychlorinated biphenyls were administered intraperitoneally at a dose of 2 mg kg(-1) alone or in combination with quercetin (orally) at 50 mg kg(-1) for 25 days. Quercetin modulation of PCBs-induced gonadal toxicity was evaluated using selected oxidative stress indices, comet assay, measurement of DNA concentration and histology of the testes. Administration of PCBs alone caused a significant (P < 0.05) depletion in the total thiol level in testes of treated rats. Conversely, the levels of reactive oxygen species (ROS) and thiobarbituric acid reactive substances (TBARS) production were markedly elevated in testes of PCBs-treated rats compared with control. Further, PCBs exposure produced statistically significant increases in DNA tail migration, degraded double-stranded DNA (dsDNA) concentration and histological alterations of testes of the treated rats compared to control. Quercetin cotreatment significantly improved the testicular antioxidant status, decreased DNA fragmentation and restored the testicular histology, thus demonstrating the protective effect of quercetin in PCBs-treated rats.

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Section: Discussionmentioning

confidence: 97%

Quercetin ameliorates polychlorinated biphenyls-induced testicular DNA damage in rats

et al. 2015

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“…SOD, CAT, GST and GPx, were measured with assay kits. 39 GPx activity was measured by following the absorbance at 412 nm on the basis of the oxidation of NADPH with H 2 O 2 . CAT activity was determined by measuring the H 2 O 2 decomposition (absorbance at 240 nm).…”

Section: Determination Of Antioxidant Enzyme Activitiesmentioning

confidence: 99%

“…38 GST activity was measured on the basis of the conjugation reaction of 1-chloro-2,4-dinitrobenzene with GSH whose product has maximum absorbance at 412 nm. 39 GPx activity was measured by following the absorbance at 412 nm on the basis of the oxidation of NADPH with H 2 O 2 . 40…”

Section: Determination Of Antioxidant Enzyme Activitiesmentioning

confidence: 99%

Protective effects of lipoic acid and mesna on cyclophosphamide‐induced haemorrhagic cystitis in mice

Song

Liu

et al. 2013

Cell Biochemistry & Function

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The protective roles of lipoic acid (LA)/vitamin C (VC) and mesna on preventing cyclophosphamide (CYP)-induced haemorrhagic cystitis (HC) were investigated. Swiss mice were divided into five groups randomly. HC was induced by a single dose of CYP injection (150-mg kg(-1) bodyweight). Group I was injected with saline (four times in total) throughout as control group. Group II received CYP and three equal doses of saline. Group III received CYP and three doses of mesna, whereas Group IV (or Group V) received CYP, mesna + two doses of VC (or LA). All injections were performed intraperitoneally. After 24 h of cystitis induction, the bladders were collected for all the experiments. Histological characterization showed that CYP injection resulted in severe HC. Reactive oxygen species (ROS) and thiobarbituric acid reactive substances' levels were increased in CYP group. The activities of antioxidant enzymes, e.g. superoxide dismutase, catalase, glutathione S-transferase and glutathione peroxidase, were inhibited significantly in CYP groups, respectively. In addition, activation of c-jun N-terminal kinases (JNK) and p38 mitogen-activated protein kinase (MAPK) may be involved in the mechanism of CYP-induced HC but not extracellular signal regulated kinases (ERK). Significant suppression of p38 phosphorylation on Group V suggests that LA and mesna may have synergistic beneficial effect. In Groups III-V, all the parameters of HC and oxidative stress were inhibited significantly. Taking together, we found that these results illustrated that ROS play an important role on CYP-induced HC and the administration of LA/VC with mesna may have therapeutic potential against CYP-induced bladder HC.

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“…Polychlorinated biphenyls (PCBs) are persistent organic pollutants found in the environment and in tissue samples (Chan et al, 2010;Tilson and Kodavanti, 1997;Tilson et al, 1990). In addition, their chemical and physical stability inhibits their degradation, leading to their ubiquitous presence in the biosphere (Bushnell et al, 2002); their lipophilicity has facilitated the bioaccumulation of these compounds and their metabolites in the food chain.…”

Section: Introductionmentioning

confidence: 99%

Effects of embryonic exposure to polychlorinated biphenyls on zebrafish (Danio rerio) retinal development

Wang

Qin

et al. 2011

J of Applied Toxicology

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Polychlorinated biphenyls (PCBs) are persistent environmental pollutants that affect embryonic development. The purpose of this study was to examine the effects of embryonic exposure to PCBs on early retinal development in zebrafish, Danio rerio. Zebrafish embryos were immediately exposed to different concentrations (0, 0.125, 0.25, 0.5, 1.0 and 2.0 mg) of PCBs per liter of medium at 28.5 °C. Embryos were assessed at 30, 48, 72 and 96 h post-fertilization (hpf) for changes in embryonic survival rate, development, larval retinal morphology and ultrastructure of the retina. The results show that PCB exposure decreased the survival rate of embryos in a time- and dose-dependent manner. Embryos exposed to the higher concentrations of PCBs (0.5, 1.0 and 2.0 mg l(-1) ) displayed obvious gross morphological deformities. At 72 hpf, the retinal layer development of zebrafish was delayed at higher PCB concentrations (1.0 mg l(-1) ). At 96 hpf, irregularity of photoreceptor cells arrangement and thickening of photoreceptor and ganglionic layers were observed in PCB-treated larvae at concentrations of 0.25-1 mg l(-1) . Ultrastructural examination showed signs of growth inhibition of the photoreceptor outer segment at 0.25-1 mg l(-1) PCB exposure at 72 hpf, as well as the appearance of massive vacuoles and holes inside the outer segments in the PCB exposure group at 96 hpf. These results suggest that embryonic exposure to moderate and high levels of PCBs induced developmental deficits in zebrafish retinas, particularly in photoreceptor cells.

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Cytotoxic effects of polychlorinated biphenyl hydroquinone metabolites in rat hepatocytes

Cited by 13 publications

References 28 publications

Quercetin ameliorates polychlorinated biphenyls-induced testicular DNA damage in rats

Quercetin ameliorates polychlorinated biphenyls-induced testicular DNA damage in rats

Protective effects of lipoic acid and mesna on cyclophosphamide‐induced haemorrhagic cystitis in mice

Effects of embryonic exposure to polychlorinated biphenyls on zebrafish (Danio rerio) retinal development

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