Cytotoxic Effects of Strawberry, Korean Raspberry, and Mulberry Extracts on Human Ovarian Cancer A2780 Cells

Abstract

Reactive oxygen species are tumorigenic by their ability to increase cell proliferation, survival, and cellular migration. The purpose of the present study was to compare the antioxidant activity and cytotoxic effects of 3 berry extracts (strawberry, Korean raspberry, and mulberry) in A2780 human ovarian carcinoma cells. Except for raspberry, the ethyl acetate or methylene chloride fractions of berries containing phenolic compounds exerted dose dependent free radical scavenging activities. In the raspberry fractions, the hexane fraction also exhibited potent antioxidant activity. The cytotoxic effects of berries extracts in A2780 human ovarian carcinoma cells were measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Surprisingly, co-treatment with n-butanol (BuOH) fractions of berries showed stronger cytotoxic effects compared to the other fractions. These findings suggest that potent anticancer molecules are found in the BuOH fractions of berries that have stronger cytotoxic activity than antioxidants.

Polyphenols Extracted from Chinese Hickory (Carya cathayensis) Promote Apoptosis and Inhibit Proliferation through the p53-Dependent Intrinsic and HIF-1α-VEGF Pathways in Ovarian Cancer Cells

“…This observation suggests that even more research, such as the current study, is needed to evaluate the therapeutic utility and safety of polyphenolic compounds. CHSP is more effective in inhibiting ovarian cancer cell proliferation than paeonol [ 19 ] or extracts of strawberry, Korean raspberry, and mulberry [ 20 ]. The lower cell viabilities demonstrate that CHSP has high potential for the prevention and therapy of ovarian cancer.…”

Anticancer Effect of Black Raspberry and Apricot Juice against Colon Cancer Induced by Azoxymethane in Male Rats

“…Recent epidemiological studies revealed that regular consumption of fruits and vegetables improve overall human health and wellbeing by preventing noncommunicable diseases including several types of cancer (Siddiqui et al, 2010;Rajput and Mandal, 2012;Brown, 2012 andLee et al, 2016). The biological value of fruits and vegetables depends upon presence of bioactive constituents, especially those of antioxidant and anti-inflammatory properties.…”

Relevance of functional foods in the Mediterranean diet: the role of olive oil, berries and honey in the prevention of cancer and cardiovascular diseases

“…In endometrial cancer cell lines urolithin A obtained from black raspberries was found particularly effective in suppressing cell proliferation in a time-and dose-dependent manner, arresting the G2/M phase of the cell cycle and modulating ERa-dependent gene expression, thereby inhibiting endometrial cancer proliferation (Zhang et al, 2016). Raspberry extracts promoted anticancer activities also in human ovarian carcinoma (Kim et al, 2016, Lee et al, 2016aLee et al, 2016b), breast (Wang et al, 2013;Zhang et al, 2015;Wang, Zhu, and Marcone 2015;Ljevar et al, 2016;Park et al, 2017) and cervical cancer cells (Saini et al, 2014;Ljevar et al, 2016) as demonstrated by the reduction in cell viability and promotion of apoptotic and cytotoxic processes. Interesting results were also obtained in vulvar squamous cell carcinoma cell line, where black raspberry extract treatment efficiently upregulated caspase-14 level, suggesting its possible use in topical treatment of vulvar lesions (Joehlin-Price et al, 2014).…”