Cytotoxic effects of ZnO nanoparticles on mouse testicular cells

Han, Zhe; Yan, Qi; Ge, Wei; Liu, Zhiguo; Gurunathan, Sangiliyandi; Felici, Massiomo De; Shen, Wei; Zhang, Xifeng

doi:10.2147/ijn.s111447

Cited by 102 publications

(82 citation statements)

References 74 publications

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“…From this XRD pattern analysis, we determined the peak intensity, position and width, and full width at half maximum data. The diffraction peaks located at 32.84°, 34.52°, 36.33°, 46.63°, 57.71°, 63.96°, 68.13°, and 69.18° have been keenly indexed as hexagonal wurtzite phase of ZnO, 29,30 and it also confirms further that the synthesized nanopowder was free of impurities as it did not contain any characteristic XRD peaks other than ZnO peaks. According to the diffraction pattern, the synthesized ZnO NP diameter was 20 nm using Debye-Scherrer formula.…”

Section: Results and Discussion Characterization Of Zno Npssupporting

confidence: 55%

“…It is also evident that significant sharp absorption of ZnO indicates the monodispersed nature of the NP distribution. 26,29 The absorption spectroscopy data revealed that both the chemically and biologically prepared ZnO NPs showed similar spectral images. Figure 1B represents the XRD pattern of ZnO nanopowder.…”

Section: Results and Discussion Characterization Of Zno Npsmentioning

confidence: 98%

“…The FTIR measurements of the ZnO NPs showed peaks at 3,445 cm -1 (phosphorous compounds, secondary sulfonamide), 2,922 cm -1 (monosubstituted alkynes, β-lactones, amine salts), 1,628 cm -1 (medium charge vinyl, cis-trisubstituted), 1,387 cm -1 (amide II), and 1,111 cm -1 (monosubstituted alkyne). 29 The peaks observed at 3,445 and 1,111 cm -1 indicate that O-H stretching and deformation, respectively assigned to the water adsorption on the metal surface. The peaks at 1,628 and 439 cm -1 correspond to stretching vibration of C−N and OH, respectively.…”

Section: Results and Discussion Characterization Of Zno Npsmentioning

confidence: 99%

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Zinc oxide nanoparticles induce apoptosis and autophagy in human ovarian cancer cells

Bai

Zhang

Zhang³

et al. 2017

IJN

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227

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BackgroundZinc oxide nanoparticles (ZnO NPs) are frequently used in industrial products such as paint, surface coating, and cosmetics, and recently, they have been explored in biologic and biomedical applications. Therefore, this study was undertaken to investigate the effect of ZnO NPs on cytotoxicity, apoptosis, and autophagy in human ovarian cancer cells (SKOV3).MethodsZnO NPs with a crystalline size of 20 nm were characterized with various analytical techniques, including ultraviolet-visible spectroscopy, X-ray diffraction, transmission electron microscopy, Fourier transform infrared spectroscopy, and atomic force microscopy. The cytotoxicity, apoptosis, and autophagy were examined using a series of cellular assays.ResultsExposure of cells to ZnO NPs resulted in a dose-dependent loss of cell viability, and the characteristic apoptotic features such as rounding and loss of adherence, enhanced reactive oxygen species generation, and loss of mitochondrial membrane potential were observed in the ZnO NP-treated cells. Furthermore, the cells treated with ZnO NPs showed significant double-strand DNA breaks, which are gained evidences from significant number of γ-H2AX and Rad51 expressed cells. ZnO NP-treated cells showed upregulation of p53 and LC3, indicating that ZnO NPs are able to upregulate apoptosis and autophagy. Finally, the Western blot analysis revealed upregulation of Bax, caspase-9, Rad51, γ-H2AX, p53, and LC3 and downregulation of Bcl-2.ConclusionThe study findings demonstrated that the ZnO NPs are able to induce significant cytotoxicity, apoptosis, and autophagy in human ovarian cells through reactive oxygen species generation and oxidative stress. Therefore, this study suggests that ZnO NPs are suitable and inherent anticancer agents due to their several favorable characteristic features including favorable band gap, electrostatic charge, surface chemistry, and potentiation of redox cycling cascades.

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Section: Results and Discussion Characterization Of Zno Npssupporting

confidence: 55%

Section: Results and Discussion Characterization Of Zno Npsmentioning

confidence: 98%

Section: Results and Discussion Characterization Of Zno Npsmentioning

confidence: 99%

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Zinc oxide nanoparticles induce apoptosis and autophagy in human ovarian cancer cells

Bai

Zhang

Zhang³

et al. 2017

IJN

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227

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“…106 Generally, silver, GO, zinc oxide, and PdNPs are known to induce ROS generation and eventually cause cell death. 36,48,61,62 TSA induced apoptosis in breast cancer cells through mitochondrial respiratory chains and increased ROS generation. 63 Therefore, the combination of both rGO-Ag and TSA causes enhanced production of ROS and is responsible for cell death.…”

mentioning

confidence: 99%

Novel biomolecule lycopene-reduced graphene oxide-silver nanoparticle enhances apoptotic potential of trichostatin A in human ovarian cancer cells (SKOV3)

Zhang¹,

Huang²,

Zhang³

et al. 2017

IJN

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Background Recently, there has been much interest in the field of nanomedicine to improve prevention, diagnosis, and treatment. Combination therapy seems to be most effective when two different molecules that work by different mechanisms are combined at low dose, thereby decreasing the possibility of drug resistance and occurrence of unbearable side effects. Based on this consideration, the study was designed to investigate the combination effect of reduced graphene oxide-silver nanoparticles (rGO-AgNPs) and trichostatin A (TSA) in human ovarian cancer cells (SKOV3). Methods The rGO-AgNPs were synthesized using a biomolecule called lycopene, and the resultant product was characterized by various analytical techniques. The combination effect of rGO-Ag and TSA was investigated in SKOV3 cells using various cellular assays such as cell viability, cytotoxicity, and immunofluorescence analysis. Results AgNPs were uniformly distributed on the surface of graphene sheet with an average size between 10 and 50 nm. rGO-Ag and TSA were found to inhibit cell viability in a dose-dependent manner. The combination of rGO-Ag and TSA at low concentration showed a significant effect on cell viability, and increased cytotoxicity by increasing the level of malondialdehyde and decreasing the level of glutathione, and also causing mitochondrial dysfunction. Furthermore, the combination of rGO-Ag and TSA had a more pronounced effect on DNA fragmentation and double-strand breaks, and eventually induced apoptosis. Conclusion This study is the first to report that the combination of rGO-Ag and TSA can cause potential cytotoxicity and also induce significantly greater cell death compared to either rGO-Ag alone or TSA alone in SKOV3 cells by various mechanisms including reactive oxygen species generation, mitochondrial dysfunction, and DNA damage. Therefore, this combination chemotherapy could be possibly used in advanced cancers that are not suitable for radiation therapy or surgical treatment and facilitate overcoming tumor resistance and disease progression.

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“…In previous studies, it was reported that the cytotoxic and genotoxic activities increased in various cells such as MCF-7 (Elavarasan et al, 2017) and MDA-MB-231 human breast cancer cells (Roshini et al, 2017) treated with ZnO alone. In a research carried out on mouse testicular cells, it was pronounced that ZnO NPs induced apoptosis through DNA damage caused by reactive oxygen species (Han et al, 2016). There are ZnO NPs induced genotoxicity studies in the literature.…”

Section: Discussionmentioning

confidence: 99%

The protective role of resveratrol against zinc oxide induced nanotoxicity

Emsen

Türkez

2017

Anatolian Journal of Botany

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Zinc oxide (ZnO) is a compound that has harmful effects as well as being used in many different areas. Numerous studies have been carried out to minimize the toxic effects of ZnO nanoparticles (NPs). In the present study, the protective role of resveratrol (RSV), a potent antioxidant polyphenol substance, was examined against ZnO-induced nanotoxicity on human pulmonary alveolar epithelial cells (HPAEpiC). In this context, the cytotoxic and genotoxic effects of different concentrations of RSV (5, 10, 20 mg/L) and ZnO NPs on the cells were measured alone and in combination. At the same time, the effects of aforementioned applications on the total antioxidant capacity (TAC) level in HPAEpiC were assessed. The results obtained showed that ZnO NPs alone significantly increased cytotoxicity and genotoxicity on cells compared to negative control (control (-)). In the experiments performed with RSV + ZnO NP combination, cytotoxic and genotoxic activity decreased at the level of p < 0.05 especially at 20 mg/L application of RSV. When the level of TAC in cells was examined, a concentration-dependent increase was detected between TAC and RSV. It was determined that ZnO NPs reduced the TAC level statistically (p < 0.05) in comparison with control (-). In conclusion, the present study revealed that RSV, a natural antioxidant, showed protective property against genotoxic and cytotoxic damage induced by ZnO NPs on HPAEpiC.

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Cytotoxic effects of ZnO nanoparticles on mouse testicular cells

Cited by 102 publications

References 74 publications

Zinc oxide nanoparticles induce apoptosis and autophagy in human ovarian cancer cells

Zinc oxide nanoparticles induce apoptosis and autophagy in human ovarian cancer cells

Novel biomolecule lycopene-reduced graphene oxide-silver nanoparticle enhances apoptotic potential of trichostatin A in human ovarian cancer cells (SKOV3)

The protective role of resveratrol against zinc oxide induced nanotoxicity

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