Cytotoxic gold(<scp>iii</scp>) complexes incorporating a 2,2′:6′,2′′-terpyridine ligand framework – the impact of the substituent in the 4′-position of a terpy ring

Czerwińska, Katarzyna; Golec, Marlena; Skonieczna, Magdalena; Palion-Gazda, Joanna; Zygadło, Dorota; Szłapa-Kula, Agata; Krompiec, Stanisław; Szurko, Agnieszka

doi:10.1039/c6dt04584g

Cited by 31 publications

(25 citation statements)

References 54 publications

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“…1a, b and c). Within the apoptotic fraction, apoptosis assays together with DNA staining distinguished necrotic from apoptotic cells and early from late apoptosis [11]. In control cultures the apoptotic fraction did not exceed 10% of the total cells for early and 15% for late apoptosis, and necrosis was almost undetectable (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Flow cytometric analysis (Aria III, Becton Dickinson) using the FITC configuration (488 nm excitation; emission: LP mirror 503, BP filter 530/30) and the PE configuration (547 nm excitation; emission: 585 nm) was performed immediately and at least 10,000 cells were counted. Cells were classified as necrotic (PI positive and Annexin-V negative; late apoptotic (PI positive and Annexin-V positive); early apoptotic (PI negative and Annexin-V positive); or normal (PI negative and Annexin-V negative) [11].…”

Section: Methodsmentioning

confidence: 99%

“…After incubation in darkness at 37 °C for 30 min the cells were washed with PBS and kept in the dark for 15 min on ice (DCFH-DA and CellROX Green) or at room temperature (MitoSOX Red and DAF-FM). Fluorescence was measured using the FITC configuration for DCFH-DA, CellROX Green and DAF-FM or the PE configuration for MitoSOX Red [11]. At least 10,000 cells were counted.…”

Section: Methodsmentioning

confidence: 99%

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The adipokine vaspin reduces apoptosis in human hepatocellular carcinoma (Hep-3B) cells, associated with lower levels of NO and superoxide anion

Skonieczna

Hudy

Hejmo

et al. 2019

BMC Pharmacol Toxicol

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Background Among adipose-derived factors, adipocytokines play roles as hormones and signaling mediators for apoptotic pathway. Among of them, vaspin, regulates the metabolism of adipose tissue itself as an endocrine organ, and stimulates adipocytes to maturation, differentiation, etc. Damaged adipocytes, present in obesity and hepatocellular carcinoma (HCC) respond with over-production of inflammatory cytokines. Such pro-inflammatory stimulation remains under adipokine control. Pro-inflammatory pathways are connected to oxidative stress and apoptosis, reported as co-existing with an elevated level of some adipokines in cancer cell lines. However, some hormones, such as vaspin, reduce apoptosis, have anti-inflammatory and anti-oxidative roles in cancer cell lines. Methods Hep-3B cells were cytometrically evaluated under vaspin treatment for reactive oxygen species (ROS) and apoptosiss induction. The statistical significant changes to the untreated controls was calculated by T-tests (indicated at value p < 0.05). Results Here we studied the production of reactive oxygen and nitrogen species in cells of HCC line Hep-3B after vaspin treatment. A decreased level of nitric oxide and superoxide anion 24 h after vaspin addition at 5 ng/ml was correlated with restricted, to the physiological level, apoptosis. A protective role of vaspin was displayed as enhanced cell viability and proliferation, which could be a poor prognostic in liver cancer. Conclusions Apoptosis was suppressed after vaspin treatment, together with low levels of nitric oxide and superoxide anions.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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The adipokine vaspin reduces apoptosis in human hepatocellular carcinoma (Hep-3B) cells, associated with lower levels of NO and superoxide anion

Skonieczna

Hudy

Hejmo

et al. 2019

BMC Pharmacol Toxicol

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“…After treatment, the cells were collected and washed with PBS twice. The cells were stained with PI and Annexin V-FITC and then analyzed using a CytoFLEX analyzer (Beckman Coulter) (for PI, Ex = 535 nm and Em = 615 nm; Annexin V-FITC, Ex = 488 nm and Em = 525 nm) [31].…”

Section: Apoptotic Cell Death Analysismentioning

confidence: 99%

Designing Aptamer-Gold Nanoparticle-Loaded pH-Sensitive Liposomes Encapsulate Morin for Treating Cancer

et al. 2020

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This study proposes the synthesis of a type of anticancer nanoparticle, aptamers and Au nanoparticle (Apt-Au)modified Morin pH-sensitive liposome (MSL), which exhibits targeting properties. Tumors are difficult to cure because their microenvironment varies from that of normal tissue; its pH is lower than that of normal tissue, which generally impedes the effectiveness of drugs. Thus, pH-responsive drugs have attracted extensive attention. Gold nanoparticles (AuNPs) show potential as drug carriers because of their small size, good biocompatibility, easy surface modification, and strong cell penetration. Apt-Au@MSL exhibits excellent monodispersity and tumor-targeting properties and can be released in partly acidic environment via dialysis. We screened our model cancer cell by MTT assay and found that SGC-7901 cells can effectively suppress proliferation. In vivo results demonstrate that the administration of Apt-Au@MSL could inhibit tumor growth in xenograft mouse models. H&E staining and TUNEL assay further confirmed that Apt-Au@MSL can promote tumor apoptosis. Apt-Au@MSL may induce apoptosis by triggering overproduction of reactive oxygen species (ROS) and regulating multiple signal crosstalk. Both blood biochemistry tests and H&E staining suggested that these materials exhibit negligible acute toxicity and good biocompatibility in vivo. With its powerful function, Apt-Au@MSL can be used as a target-based anticancer material for future clinical cancer treatment.

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“…Among engineered materials various compounds are used including metals: silver (Ag) [12,13], gold (Au) [13,14], copper (Cu) [14], zinc (Zn) [15,16], gallium (Al) [17], metal oxides [16], and many others [1,18,19]. Based on the physical and chemical approaches of metal-based nanoparticles, numerous features can be used in their applications, including shape recognition, paramagnetic properties, biocompatibility, fluorescence, and optical density [19].…”

Section: Introductionmentioning

confidence: 99%

Biological Activity of Silver Nanoparticles and Their Applications in Anticancer Therapy

Skonieczna¹,

Hudy²

2018

Silver Nanoparticles - Fabrication, Characterization and Applications

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Nanotechnology delivers materials and nanoparticles (NPs) with high biological potential, useful in bioengineering, nanomedicine, and human health protection. Silver nanoparticles (NPs), because of their wide spectrum of activities and physical and chemical properties, are nowadays extensively researched. However, careful studies on living organism should be performed, with strong attention to biocompatibility. Multiple cellular effects, displayed after AgNP treatments, show interesting potential of metal-based NPs, not only in bio-nanotechnology but also in molecular medicine and anticancer therapy. AgNPs are promising anticancer agents, influencing the cell cycle, inhibiting cancer proliferation, and inducing oxidative stress and propagation of programmed cellular death (apoptosis). Additionally, they protect against bacterial, fungal, and viral infections. During chemo-and radio-therapies, such antimicrobial protection will be desirable because of the decreased immunological resistance of cancer patients. In conclusion, AgNPs often present in the human environment should be studied for novel findings and better characteristic. This article discusses advantages of AgNP's "eco-friendly" production, followed by green synthesis, with particular consideration of antimicrobial and anticancer properties. Cellular processes, induced after AgNP treatments, are focused on antiproliferative, pro-oxidative, and pro-apoptotic activities of NPs.

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Cytotoxic gold(iii) complexes incorporating a 2,2′:6′,2′′-terpyridine ligand framework – the impact of the substituent in the 4′-position of a terpy ring

Cited by 31 publications

References 54 publications

The adipokine vaspin reduces apoptosis in human hepatocellular carcinoma (Hep-3B) cells, associated with lower levels of NO and superoxide anion

The adipokine vaspin reduces apoptosis in human hepatocellular carcinoma (Hep-3B) cells, associated with lower levels of NO and superoxide anion

Designing Aptamer-Gold Nanoparticle-Loaded pH-Sensitive Liposomes Encapsulate Morin for Treating Cancer

Biological Activity of Silver Nanoparticles and Their Applications in Anticancer Therapy

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