Cytotoxic, nuclear, and growth inhibitory effects of photodynamic drugs on pancreatic carcinoma cells

Ward, Amanda J.; Matthews, E. K.

doi:10.1016/0304-3835(96)04152-3

Cited by 8 publications

(4 citation statements)

References 27 publications

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“…ALA, AlPcS, hypericin, pheopharbide, Pterin and verteporfin) [37—42]. PDT might be effective for chemotherapy insensitive pancreatic cancer cells [43].…”

Section: Discussionmentioning

confidence: 99%

Effect of Photofrin-mediated photocytotoxicity on a panel of human pancreatic cancer cells

Wang

Huang

Han

et al. 2013

Photodiagnosis and Photodynamic Therapy

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Summary Background and objective Pancreatic cancer is a leading cause of cancer-related deaths in men and women. Early clinical studies suggest that photodynamic therapy (PDT) might be a useful modality in the management of this deadly disease. In this study, the photocytotoxicity of Photofrin-mediated PDT on different human pancreatic cancer cells (BxPc-3, HPAF-II, Mia PaCa-2, MPanc-96, PANC-1 and PL-45) was examined. Materials and methods After co-incubating cancer cells with Photofrin (0—10 [H9262]g/ml) for 4 h, the cells were irradiated with 0—6 J/cm2 of 630 nm light. The effect of Photofrin PDT on the survival of cells were examined using tetrazolium-based colorimetric assay and clonogenic assay. PDT-induced apoptosis was analyzed by flow cytometry. Expressions of apoptosis-related proteins were determined by western blot analysis. Results Photofrin PDT strongly inhibited the survival of pancreatic cancer cells. A small portion of cells (<15%) underwent apoptosis 24 h after PDT at LD50. Cleavage of caspase-3, caspase-8, caspase-9 and PARP after PDT were also confirmed. BxPc-3, Mia PaCa-2, MPanc-96, and PANC-1 cells were more sensitive and HPAF-II and PL-45 cells less sensitive. Conclusion Photofrin PDT can induce apoptosis and inhibit survival of human pancreatic cancer cells.

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“…ALA, AlPcS, hypericin, pheopharbide, Pterin and verteporfin) [37—42]. PDT might be effective for chemotherapy insensitive pancreatic cancer cells [43].…”

Section: Discussionmentioning

confidence: 99%

Effect of Photofrin-mediated photocytotoxicity on a panel of human pancreatic cancer cells

Wang

Huang

Han

et al. 2013

Photodiagnosis and Photodynamic Therapy

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“…Sulfonated phthalocyanines bearing a central aluminium ion have been extensively studied in vitro as well as in vivo . They display potent photodynamic activity on cell lines including G361 human melanoma cells [ 73 ], pancreatic carcinoma cells (H2T) [ 74 ], and human fibrosacroma cells (HT-1080) [ 75 ], as well as in vivo on rat (CBH rats) bearing fibrosarcoma (HSN/TC/7) [ 76 ]. These early studies indicated a strong dependence of the photodynamic efficiency on the degree of sulfonation, later systematically assessed by Chan et al .…”

Section: Phthalocyaninesmentioning

confidence: 99%

Like a Bolt from the Blue: Phthalocyanines in Biomedical Optics

et al. 2011

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The purpose of this review is to compile preclinical and clinical results on phthalocyanines (Pcs) as photosensitizers (PS) for Photodynamic Therapy (PDT) and contrast agents for fluorescence imaging. Indeed, Pcs are excellent candidates in these fields due to their strong absorbance in the NIR region and high chemical and photo-stability. In particular, this is mostly relevant for their in vivo activation in deeper tissular regions. However, most Pcs present two major limitations, i.e., a strong tendency to aggregate and a low water-solubility. In order to overcome these issues, both chemical tuning and pharmaceutical formulation combined with tumor targeting strategies were applied. These aspects will be developed in this review for the most extensively studied Pcs during the last 25 years, i.e., aluminium-, zinc- and silicon-based Pcs.

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“…However, mitochondrial damage may not necessarily be equivalent with a reduced energy charge and ATP level because substantially higher doses of light were needed for a reduction of the energy charge than for inhibition of oxygen consumption ( 1 15). The ALA PDT may exert its effect through formation of singlet oxygen (lo2) (152) although other reactive oxygen species may also be involved (100). The range of action of lo2 in cells has been estimated to be approximately 0.01-0.02 pm (153) but may be even shorter if the photoactivated dye producing lo2 is closely associated with a target rich in molecules that can react with or quench lo2.…”

Section: Mechanisms and Efficiency Of Ala-derived Photosensitization mentioning

confidence: 99%

5‐Aminolevulinic Acid‐Based Photodynamic Therapy: Principles and Experimental Research

Peng

Berg

Moan

et al. 1997

Photochem & Photobiology

591

496

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Cytotoxic, nuclear, and growth inhibitory effects of photodynamic drugs on pancreatic carcinoma cells

Cited by 8 publications

References 27 publications

Effect of Photofrin-mediated photocytotoxicity on a panel of human pancreatic cancer cells

Effect of Photofrin-mediated photocytotoxicity on a panel of human pancreatic cancer cells

Like a Bolt from the Blue: Phthalocyanines in Biomedical Optics

5‐Aminolevulinic Acid‐Based Photodynamic Therapy: Principles and Experimental Research

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