Cytotoxic Potential of Nickel Oxide Nanoparticles Functionalized with Glutamic Acid and Conjugated with Thiosemicarbazide (NiO@Glu/TSC) Against Human Gastric Cancer Cells

Hosseinkhah, Maryam; Ghasemian, Reza; Shokrollahi, Faezeh; Mojdehi, Samira Rezaei; Noveiri, Mahboubeh Jahani Sayyad; Hedayati, Mohammad; Rezaei, Marjan; Salehzadeh, Ali

doi:10.1007/s10876-021-02124-2

Cited by 27 publications

(11 citation statements)

References 22 publications

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“…Finally, the optical absorbance of the wells was measured at 570 nm by a Bio-Rad microplate reader. The inhibitory percentage of the nanoparticles for the studied cell lines was measured using the following formula 30 . The inhibitory effect of Cisplatin, as a standard anticancer drug, for the AGS cell line was also determined.…”

Section: Methodsmentioning

confidence: 99%

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Biosynthesized BiFe2O4@Ag nanoparticles mediated Scenedesmus obliquus induce apoptosis in AGS gastric cancer cell line

Shamsi,

Yari,

Salehzadeh

2024

Sci Rep

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The use of magnetic metal nanoparticles has been considered in cancer treatment studies. In this study, BiFe2O4@Ag nanoparticles were synthesized biologically by Scenedesmus obliquus for the first time and their anticancer mechanism in a gastric cancer cell line was characterized. The physicochemical properties of the nanoparticles were evaluated by fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), dynamic Light Scattering (DLS), and zeta potential analyses. Cell viability and nuclear damage were investigated by the MTT and Hoechst staining assays, respectively. Flow cytometry analysis was performed to determine the frequency of the necrotic and apoptotic cells as well as cell cycle analysis of the nanoparticles-treated cells. Physicochemical characterization showed that the synthesized particles were spherical, without impurities, in a size range of 38–83 nm, with DLS size and zeta potential of 295.7 nm and -27.7 mV, respectively. BiFe2O4@Ag nanoparticles were considerably more toxic for the gastric cancer cells (AGS cell line) than HEK293 normal cells with IC50 of 67 and 117 µg/ml, respectively. Treatment of AGS cells with the nanoparticles led to a remarkable increase in the percentage of late apoptosis (38.5 folds) and cell necrosis (13.4 folds) and caused cell cycle arrest, mainly at the S phase. Also, nuclear fragmentation and apoptotic bodies were observed in the gastric cancer cells treated with the nanoparticles. This study represents BiFe2O4@Ag as a novel anticancer candidate against gastric cancer that can induce cell apoptosis through DNA damage and inhibition of cell cycle progression.

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Section: Methodsmentioning

confidence: 99%

“…Untreated cells were regarded as the control. After washing the cell monolayer with PBS, the cells were examined under a fluorescent microscope (Zeiss, Wetzlar, Germany) 30 .…”

Section: Methodsmentioning

confidence: 99%

Biosynthesized BiFe2O4@Ag nanoparticles mediated Scenedesmus obliquus induce apoptosis in AGS gastric cancer cell line

Shamsi,

Yari,

Salehzadeh

2024

Sci Rep

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“…The plates were stored at room temperature for 30 min and the optical absorption of the wells was measured at 570 nm (Bio-Rad, Hercules, microplate reader). The 50% inhibitory concentration (IC 50 ) of the NPs was calculated by GraphPad Prism software (version 9.5.1, USA), and the percentage of inhibited cells as was calculated according to the following formula [12][13]:…”

Section: Mtt Assaymentioning

confidence: 99%

Genetic Insights and Therapeutic Potential for Colorectal cancer: Mutation Analysis of KRAS Gene and Efficacy of Oleuropein-Conjugated Iron Oxide Nanoparticles

Mehdinejad,

Peymani,

Salehzadeh

et al. 2024

Preprint

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This study aimed to address the challenges of treating advanced stages of colon cancer (CRC) by exploring potential therapeutic options. The research focused on the genetic aspects of CRC, specifically the mutation rate of the KRAS gene, along with other genes like TTN, APC, MUC16, and TP53, using the TCGA dataset. Additionally, the study investigated the efficacy of Oleuropein, a polyphenolic compound found in olives, in combating CRC by using iron oxide nanoparticles coated with glucose and conjugated with Oleuropein. The study characterized the physicochemical properties of the nanoparticles and the cytotoxic effects of the nanoparticles were evaluated on CRC and normal fibroblast cell lines, demonstrating significantly higher cytotoxicity against CRC cells compared to normal cells. Furthermore, the study analyzed gene expression changes using the GSE124627 dataset to understand the influence of KRAS alterations. It identified numerous upregulated and downregulated genes in KRAS-overexpressing samples, suggesting their involvement in critical cancer-related pathways. These findings suggest that KRAS-influenced genes could serve as potential therapeutic targets for CRC treatment. The study also examined the expression levels of identified genes in CRC samples compared to normal samples. Among the upregulated genes, 22 showed significant increases in cancer samples, while 14 downregulated genes exhibited decreased expression in both KRAS-influenced and cancer samples. Cox regression analysis identified specific upregulated genes, including ANKZF1, SNAI1, PPFIA4, SIX4, and NOTUM, associated with poor prognosis. Kaplan-Meier analysis further confirmed the correlation between increased expression of these genes and higher patient mortality rates. In conclusion, this study provided valuable insights into the genetic aspects of CRC and potential therapeutic strategies. The use of Oleuropein-conjugated iron oxide nanoparticles showed promising cytotoxic effects on colon cancer cells. These findings contribute to advancing our understanding of CRC and offer potential targets for further investigation and the development of novel therapeutic approaches.

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“…Consequently, cobalt and nickel oxide NPs that were synthesized via chemical methods have shown dose-dependent cytotoxicity against AGS gastric cancer cell line. However, both cobalt and nickel oxide NPs exhibited slight toxicity towards normal fibroblast cells (L929) [90,108]. Nevertheless, silver NPs are one of the most widely used among many metallic NPs as an anticancer agent.…”

Section: Metallic Nanoparticlesmentioning

confidence: 99%

Novel Drug Delivery Systems as an Emerging Platform for Stomach Cancer Therapy

et al. 2022

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Cancer has long been regarded as one of the world’s most fatal diseases, claiming the lives of countless individuals each year. Stomach cancer is a prevalent cancer that has recently reached a high number of fatalities. It continues to be one of the most fatal cancer forms, requiring immediate attention due to its low overall survival rate. Early detection and appropriate therapy are, perhaps, of the most difficult challenges in the fight against stomach cancer. We focused on positive tactics for stomach cancer therapy in this paper, and we went over the most current advancements and progressions of nanotechnology-based systems in modern drug delivery and therapies in great detail. Recent therapeutic tactics used in nanotechnology-based delivery of drugs aim to improve cellular absorption, pharmacokinetics, and anticancer drug efficacy, allowing for more precise targeting of specific agents for effective stomach cancer treatment. The current review also provides information on ongoing research aimed at improving the curative effectiveness of existing anti-stomach cancer medicines. All these crucial matters discussed under one overarching title will be extremely useful to readers who are working on developing multi-functional nano-constructs for improved diagnosis and treatment of stomach cancer.

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Cytotoxic Potential of Nickel Oxide Nanoparticles Functionalized with Glutamic Acid and Conjugated with Thiosemicarbazide (NiO@Glu/TSC) Against Human Gastric Cancer Cells

Cited by 27 publications

References 22 publications

Biosynthesized BiFe2O4@Ag nanoparticles mediated Scenedesmus obliquus induce apoptosis in AGS gastric cancer cell line

Biosynthesized BiFe2O4@Ag nanoparticles mediated Scenedesmus obliquus induce apoptosis in AGS gastric cancer cell line

Genetic Insights and Therapeutic Potential for Colorectal cancer: Mutation Analysis of KRAS Gene and Efficacy of Oleuropein-Conjugated Iron Oxide Nanoparticles

Novel Drug Delivery Systems as an Emerging Platform for Stomach Cancer Therapy

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