Cytotoxicity against various cell lines of lipopolysaccharides purified from bacteroides, fusobacterium, and veillonella isolated from infected root canals

Horiba, Naoki; Maekawa, Yoichi; Abe, Yoshie; Ito, Masato; Matsumoto, Toru; Nakamura, Hiroshi; Ozeki, Masami

doi:10.1016/s0099-2399(89)80195-5

Cited by 25 publications

(23 citation statements)

References 12 publications

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“…The biological activities of smooth-type LPS from F. nucleatum were shown in this study to be weaker than, but still comparable to, those of Salmonella or E. coli LPS, and these results are in agreement with previous reports on F nucleatum LPS from the aqueous phase (11,12,18). This is because the structural principle of enterobacterial LPS and F. nucleatum LPS is similar except for the amide-linked 3-hydroxy fatty acid (9).…”

Section: Discussionsupporting

confidence: 92%

“…Among such studies, there have been few reports on the LPS of F. nucleatum, although F. nucleatum is one of the predominant species in the gingival pocket. Hase et al (9) determined the chemical structure of the lipid A portion of F. nucleatum LPS prepared from the aqueous phase of hot-phenol water extraction, and the biological activities of the LPS have been investigated by several groups (11,23). The structure and the biological activities of F. nucleatum lipid A were found to be similar to those of E. coli, although the amide-linked fatty acid was a longer one (3-hydroxy palmitic acid).…”

Section: Human Gingival Fibroblastsmentioning

confidence: 99%

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Extraction and Characterization of the Smooth‐Type Lipopolysaccharide from Fusobacterium nucleatum JCM 8532 and Its Biological Activities

Onoue

Niwa

Isshiki

et al. 1996

Microbiology and Immunology

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The lipopolysaccharide (LPS) of Fusobacterium nucleatum JCM 8532 was isolated by hot-phenol water extraction. Most of the LPS was extracted in the phenolic phase and shown to be the smooth-type, whereas the aqueous phase contained mainly rough-type LPS. The chemical composition of the LPS was similar to that reported in other studies, but D-quinovosamine, which may be a major component of O-antigenic polysaccharide, and 3-deoxy-D-manno-2-octulosonic acid (Kdo) were detected for the first time by gas chromatography-mass spectrometry. The biological activities of smooth-type LPS, including limulus activity, lethal toxicity, pyrogenicity, and B lymphocyte mitogenicity, were comparable to those of enterobacterial LPS. Smooth-type LPS inhibited the cell growth and DNA synthesis of adult and fetal human gingival fibroblasts in a dose-dependent manner, suggesting that LPS may play a role in the occurrence of human gingivitis.

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Section: Discussionsupporting

confidence: 92%

Section: Human Gingival Fibroblastsmentioning

confidence: 99%

Extraction and Characterization of the Smooth‐Type Lipopolysaccharide from Fusobacterium nucleatum JCM 8532 and Its Biological Activities

Onoue

Niwa

Isshiki

et al. 1996

Microbiology and Immunology

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“…Endotoxin is one of the most important virulent factors involved in the development of periapical inflammation (3)(4)(5), activating immune-competent cells and leading to the release of a variety of proinflammatory mediators (5)(6)(7)(8).…”

mentioning

confidence: 99%

Comparison of Endotoxin Levels Found in Primary and Secondary Endodontic Infections

Gomes

Endo

Martinho

2012

Journal of Endodontics

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“…In addition, our results suggest that fusobacterial LPS could have caused the lethal effect in mice. On the other hand, F. nucleatum possesses a potent LPS capable to induce cutaneous inflammatory reactions in rabbits (31), produces haemolysis (32) and has cytotoxic effects to L-929 and WI-38 cells (33). LPS is involved in pathogenesis of inflammatory reaction in mice, because it activates the complement system (34) or induces leukocyte migration (35).…”

Section: Discussionmentioning

confidence: 99%

“…The pathogenic potential of F. nucleatum has been studied in vitro (7,14,33). However, there are few reports on virulence of this microorganism recovered from humans and Cebus apella monkeys, using animal models.…”

Section: Introductionmentioning

confidence: 99%

Virulence of oral Fusobacterium nucleatum from humans and non-human primates in mice

Júnior¹,

Luvizotto²,

Ávila-Campos³

2000

Braz. J. Microbiol.

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The lethal action in Balb/c mice of 80 oral Fusobacterium nucleatum recovered from patients with adult periodontitis, healthy subjects or Cebus apella monkeys was studied. Mice were inoculated intraperitoneally with each bacterial inoculum of 5 x 10 8 CFU/ml. All the clinical isolates induced weight and coordinated movements loss. Pathological alterations in liver, CNS, heart, and kidney with inflammatory reactions or vascular congestion were observed. Of all the tested F. nucleatum isolates, 61.2% from periodontal patients, 57.1% from healthy subjects and 60% from monkeys, were capable of killing the mice in 48h. The clinical isolates were significantly more pathogenic than F. nucleatum ATCC 10953 or ATCC 25586. B. fragilis ATCC 23745 showed lethality against control mice. Our results suggest that LPS could be involved in lethal action against mice and it may play an important role in producing tissue damage or death of mice.

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Cytotoxicity against various cell lines of lipopolysaccharides purified from bacteroides, fusobacterium, and veillonella isolated from infected root canals

Cited by 25 publications

References 12 publications

Extraction and Characterization of the Smooth‐Type Lipopolysaccharide from Fusobacterium nucleatum JCM 8532 and Its Biological Activities

Extraction and Characterization of the Smooth‐Type Lipopolysaccharide from Fusobacterium nucleatum JCM 8532 and Its Biological Activities

Comparison of Endotoxin Levels Found in Primary and Secondary Endodontic Infections

Virulence of oral Fusobacterium nucleatum from humans and non-human primates in mice

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