Cytotoxicity and accumulation of ergot alkaloids in human primary cells

Mulac, Dennis; Humpf, Hans‐Ulrich

doi:10.1016/j.tox.2011.01.019

Cited by 80 publications

(103 citation statements)

References 24 publications

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“…was given to the fasted animals to induce gastric injury. In the drug-pretreated groups, the animals were given SEGA (3a) (50,20,10,5,3, and 1 mg⅐kg Ϫ1 b.w. ), intraperitoneally 30 min prior indomethacin treatment.…”

Section: Determination Of In Vitro Antioxidant Property By Following mentioning

confidence: 99%

Tryptamine-Gallic Acid Hybrid Prevents Non-steroidal Anti-inflammatory Drug-induced Gastropathy

Pal

Dey

Alam

et al. 2012

Journal of Biological Chemistry

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Background: Non-steroidal anti-inflammatory drugs (NSAIDs) induce gastropathy by promoting mitochondrial pathology, oxidative stress, and apoptosis in gastric mucosal cells. Results: We have synthesized SEGA (3a), a tryptamine-gallic acid hybrid, which prevents NSAID-induced gastropathy by preventing mitochondrial oxidative stress, dysfunction, and apoptosis. Conclusion: SEGA (3a) bears an immense therapeutic potential against NSAID-induced gastropathy. Significance: This novel molecule is a significant addition in the discovery of gastroprotective drugs.

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Section: Determination Of In Vitro Antioxidant Property By Following mentioning

confidence: 99%

Tryptamine-Gallic Acid Hybrid Prevents Non-steroidal Anti-inflammatory Drug-induced Gastropathy

Pal

Dey

Alam

et al. 2012

Journal of Biological Chemistry

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“…Classes como a dos alcaloides, antraquinonas, cumarinas, fenóis, flavonoides, heterosídeos antraquinônicos e cardioativos, taninos e terpenos estão entre as responsáveis pela ação alelopática, devido a toxicidade sobre as células vegetais, alterando a permeabilidade da membrana das células meristemáticas ou atuando em seu citoesqueleto, por exemplo, levando-as à morte (Weir et al 2004;Fujii and Hiradate 2007;Adams et al 2011;Mulac and Humpf 2011). Desta maneira, estes metabólitos podem afetar negativamente a germinação ou desenvolvimento das plântulas, por exemplo.…”

Section: Estudos Deunclassified

Potencial alelopático do extrato etanólico de Anacardium humile (cajuzinho-do-cerrado) na germinação e formação de plântulas de alface, tomate e fedegoso

Pereira

Oliveira

Matias

et al. 2018

G. Sci.

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O gênero Anacardium, Anacardiaceae, destaca-se em relação aos demais gêneros desta família devido à presença de compostos fenólicos, que possuem inúmeras aplicações e usos populares. Uma das espécies, investigada pelos diferentes usos e potencialidades de utilização, é Anacardium humille (cajuzinho-do-cerrado). Porém pouco se sabe sobre sua ação alelopática. Desta maneira, o presente trabalho teve por objetivo qualificar a composição química das folhas e avaliar os possíveis efeitos alelopáticos do extrato etanólico, na germinação de sementes e formação de plântulas de espécies-alvo, alface, tomate e fedegoso. Para os bioensaios em câmara de germinação, alface e tomate, foram utilizados os extratos nas concentrações de 0 (controle), 25, 50, 100, 150 e 200 mg mL-1 e fedegoso, 100 e 200 mg mL-1. A avaliação em casa de vegetação utilizou bandejas de isopor com substratos nas proporções de 0, 5, 10 e 20%. O delineamento experimental foi o inteiramente casualizado. O extrato apresentou diferentes classes de metabólitos, com compostos fenólicos e antraquinonas em maior intensidade. Os resultados demonstraram efeito alelopático na germinação e no crescimento das espécies testadas em câmara de germinação e casa de vegetação, em diferentes graus, indicando que o mecanismo de ação da alelopatia está relacionado com os principais aleloquímicos encontrados, os ácidos fenólicos e as antraquinonas, detectados nas folhas, demonstrando seu potencial de uso.

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“…Animals are infected through consumption of contaminated feed. This is a widespread phenomenon in livestock leading to adverse effects ranging from weight loss to death of infected animals 4,5,6,2,7 .…”

Section: Introductionmentioning

confidence: 99%

Development of Suspension Polymerized Molecularly Imprinted Beads with Metergoline as Template and Application in a Solid-Phase Extraction Procedure toward Ergot Alkaloids

Lenain

Mavungu²,

Dubruel³

et al. 2012

Anal. Chem.

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The first successfully developed molecularly imprinted polymer toward six ergot alkaloids and their respective epimers is described. A new imprinting molecule, metergoline, was used as template analogue in the production of suspension polymerized beads. These spherical particles functioned as selective sorbent in a solid-phase extraction column. The application of this column in the cleanup of barley samples prior to liquid chromatography coupled with tandem mass spectrometry allowed simple and cost-efficient sample preparation. The performance of the imprinted polymer and a non-imprinted control polymer was evaluated. This includes determination of the recovery values and the matrix effect of each of the 12 tested ergot alkaloids as well as a crossreactivity study with 25 common mycotoxins. The binding isotherms were obtained for metergoline, thus allowing comparison with other (imprinted) sorbents. A comparison between bulk and suspension polymerization is provided to determine the appropriate production technique. E rgot alkaloids are mycotoxins produced by Claviceps species, specifically Claviceps purpurea. These fungi can be found on cereals and produce sclerotia, which vary in composition and concentration of ergot alkaloids. The sclerotia are harvested together with the grains and only up to 80% of the kernels can be removed with the mechanical separation techniques currently available. Especially in periods of drought these techniques become unreliable as smaller sclerotia, similar in size to the grains, are produced. Generally, sclerotia are also broken during transport, allowing them to enter the food chain more easily.These ergot alkaloids possess toxicity due to interactions with α-adrenergic, serotinergic, and dopaminergic receptors. 1−3 Excess intake by humans can cause nausea, convulsions, hallucinations, and vasoconstriction and can even lead to gangrenous symptoms and abortion. Animals are infected through consumption of contaminated feed. This is a widespread phenomenon in livestock leading to adverse effects ranging from weight loss to death of infected animals. [4][5][6]2,7 The European Food Safety Authority (EFSA) stated in 2005 that the degree of variability in ergot alkaloid pattern in relation to fungal species, host plant, and geographical distribution is not known. 5 Six ergot alkaloidsergometrine, ergosine, ergotamine, ergocristine, ergocryptine, and ergocornine which are considered the most important ones according to EFSA, are depicted in Figure 1. An isomerization from the biologically active R-form to the biologically inactive S-form occurs at the C 8 stereogenic center of the tetracyclic ergoline structure in a reversible epimerization reaction. 8,9,6 The suffix "-ine" is used in the nomenclature if the side chain is present in the R-form at the C 8 stereogenic center, for example, ergotamine. The side chain is present in the S-orientation in the corresponding epimer which is expressed by the suffix "-inine", for example, ergotaminine. This reaction is promoted in humid, aqueous,...

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Cytotoxicity and accumulation of ergot alkaloids in human primary cells

Cited by 80 publications

References 24 publications

Tryptamine-Gallic Acid Hybrid Prevents Non-steroidal Anti-inflammatory Drug-induced Gastropathy

Tryptamine-Gallic Acid Hybrid Prevents Non-steroidal Anti-inflammatory Drug-induced Gastropathy

Potencial alelopático do extrato etanólico de Anacardium humile (cajuzinho-do-cerrado) na germinação e formação de plântulas de alface, tomate e fedegoso

Development of Suspension Polymerized Molecularly Imprinted Beads with Metergoline as Template and Application in a Solid-Phase Extraction Procedure toward Ergot Alkaloids

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