Cytotoxicity and apoptotic effects of nickel oxide nanoparticles in cultured HeLa cells.

Abstract

Abstract:The aim of this study was to observe the cytotoxicity and apoptotic effects of nickel oxide nanoparticles on human cervix epithelioid carcinoma cell line (HeLa). Nickel oxide precursors were synthesized by an nickel sulphate-excess urea reaction in boiling aqueous solution. The synthesized NiO nanoparticles (<200 nm) were investigated by X-ray diffraction analysis and transmission electron microscopy techniques. For cytotoxicity experiments, HeLa cells were incubated in 50-500 μg/mL NiO for 2, 6, 12 and 16 hours. The viable cells were counted with a haemacytometer using light microscopy. The cytotoxicity was observed low in 50-200 μg/mL concentration for 16 h, but high in 400-500 μg/mL concentration for 2-6 h. HeLa cells' cytoplasm membrane was lysed and detached from the well surface in 400 μg/mL concentration NiO nanoparticles. Double staining and M30 immunostaining were performed to quantify the number of apoptotic cells in culture on the basis of apoptotic cell nuclei scores. The apoptotic effect was observed 20% for 16 h incubation.

Cytotoxic effect of inositol hexaphosphate and its Ni(II) complex on human acute leukemia Jurkat T cells

“…This information is important because free Ni(II) in solution, per se is toxic, and promotes oxidative stress, apoptosis, G2/M arrest and genotoxicity in normal rat kidney cells (Chen et al, 2010;Taira et al, 2001). Ni(II) also presents an apoptotic effect on HeLa tumor cells (Ada et al, 2010). The present study also verified that Ni(II), in the form of NiCl 2 salt, was toxic for both normal and tumor cells (Figs.…”

“…In the same way, Ni(II) has been described as an important cytotoxic agent and apoptosis inductor (Ada et al, 2010;Chen et al, 2010). In the present study, InsP 6 was used for the first time in the form of a complex with Ni(II) to enhance the efficacy of this compound as an antitumoral agent.…”

A Comparative Study of CdTe Quantum Dots and CdTe@SiO2 Nanoparticles: Fabrication and Cytotoxicity in HEK293 Cells

“…The findings were similar to Ada et al 2010. 29 The results were consisting with the findings of Lihong et al 2010.…”

“…The findings were similar to Ada et al 2010. 29 The results were consisting with the findings of Lihong et al 2010. 23 The decrease of fluorescence QY has been explained by various mechanisms mainly involving ligand exchanges and impurities were the key factor responsible for the loss of fluorescence QY.…”

Genotoxicity and oxidative stress induced by the orally administered nanosized nickel and cobalt oxides in male albino rats

“…NiO showed relatively low toxicity as compared to the other Ni compounds (Zhao et al, 2009). However, several studies have evidenced that NiO-NPs caused cytotoxic and apoptotic effects in various mammalian cell lines (Lu et al, 2008;Ada et al, 2010 andPietruska et al, 2011). Furthermore, NiO-NP-induced toxicity has been demonstrated in microorganisms such as bacteria (Baek & An, 2011), algae (Gong et al, 2011), and plants (Faisal et al, 2013).…”