Cytotoxicity and effects on inflammatory response of modified types of cellulose in macrophage-like THP-1 cells

Kollár, Péter; Závalová, Veronika Müller; Hošek, Jan; Havelka, Pavel; Sopuch, Tomáš; Karpíšek, Michal; Třetinová, Dominika; Suchý, Pavel

doi:10.1016/j.intimp.2011.02.016

Cited by 46 publications

(43 citation statements)

References 19 publications

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“…The first studies showed evidence that highly crystalline regenerated cellulose did not induce the immunological response in vivo in a canine model, as judged by the absence of foreign body reaction (Miyamoto et al 1989). These results were in accordance with in vitro studies showing that CNFs did not induce toxicity of mouse and human fibroblasts (Mathew et al 2012;Alexandrescu et al 2013;Hua et al 2014), mouse and human macrophages (Vartiainen et al 2011), human monocytic leukaemia cells (Kollar et al 2011) and human cervix carcinoma cells (Pitkänen et al 2014). Recently, in contrast, it seems that cellulose nanocrystals (CNCs) thinner and much shorter than CNFs, might be even toxic and proinflammatory, after pulmonary exposition in vivo, as judged by the accumulation of inflammatory cells within the bronchoalveolar fluid and increased production of pro-inflammatory cytokines and chemokines (Yanamala et al 2014).…”

Section: Introductionsupporting

confidence: 92%

“…These findings are in accordance with those published by Vartiainen et al (2011) who demonstrated that microfibrillated cellulose did not caused any effects on TNF-α and IL-1β expression in a mouse monocytic cell line (RAW 264.7 cells) and human monocyte derived macrophages. In addition, it has been shown that microfibrillated nanocellulose was unable to trigger the production of TNF-α by human macrophage-like THP-1 cells, nor to modify the lipopolysaccharide (LPS)-induced production of TNF-α (Kollar et al 2011), in contrast to microcrystalline nanocellulose, which significantly blocked the LPS-stimulated TNF-α secretion. Although our study should be extended to examine the effect of CNFs on the production of pro-inflammatory cytokines by isolated macrophages and dendritic cells, the findings that the secretion of both TNF-α and IL-1β in PBMNC cultures was unchanged by CNFs indicate that this wood-derived nanocellulose did not trigger the inflammatory response.…”

Section: Discussionmentioning

confidence: 99%

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Cytocompatibility and immunomodulatory properties of wood based nanofibrillated cellulose

et al. 2014

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Cellulose nanofibrils (CNFs), unique and promising natural materials have gained significant attention recently for biomedical applications, due to their special biomechanical characteristics, surface chemistry, good biocompatibility and low toxicity. However, their long bio-persistence in the organism may provoke immune reactions and this aspect of CNFs has not been studied to date. Therefore, the aim of this work was to examine and compare the cytocompatibility and immunomodulatory properties of CNFs in vitro. CNFs (diameters of 10-70 nm; lengths of a few microns) were prepared from Norway spruce (Picea abies) by mechanical fibrillation and high pressure homogenisation. L929 cells, rat thymocytes or human peripheral blood mononuclear cells (PBMNCs) were cultivated with CNFs. None of the six concentrations of CNFs (31.25 µg/ml-1 mg/ml) induced cytotoxicity and oxidative stress in the L929 cells, nor induced necrosis and apoptosis of thymocytes and PBMNCs. Higher concentrations (250 µg/ml-1 mg/ml) slightly inhibited the metabolic activities of the L929 cells as a consequence of inhibited proliferation. The same concentrations of CNFs suppressed the proliferation of PBMNCs to phytohemaglutinine, a T-cell mitogen, and the process was followed by down-regulation of interleukin-2 (IL-2) and interferon-γ production. The highest concentration of CNFs inhibited IL-17A, but increased IL-10 and IL-6 production. The secretion of pro-inflammatory cytokines, IL-1β and tumor necrosis factor-α as well as Th2 cytokine (IL-4), remained unaltered. In conclusion, the results suggest that these CNFs are cytocompatible nanomaterial, according to current ISO criteria, with non-inflammatory and nonimmunogenic properties. Higher concentrations seem to be tolerogenic to the immune system, a characteristic very desirable for implantable biomaterials.

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Section: Introductionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Cytocompatibility and immunomodulatory properties of wood based nanofibrillated cellulose

et al. 2014

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“…In our previous report, in chitosan-based matrix did not show any sign of toxicity on osteoblastic lines [39]. On the other hand, no cytotoxic effect has been reported for carboxymethyl cellulose [51]. Thus, our present results demonstrate that both PEC-US and PEC exerted no in vitro cytotoxicity on Raw 264.7 macrophages.…”

Section: Cytotoxicity Evaluationsupporting

confidence: 51%

Ultrasonic compatibilization of polyelectrolyte complex based on polysaccharides for biomedical applications

Belluzo

Medina

Cortizo

et al. 2016

Ultrasonics Sonochemistry

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a b s t r a c tIn recent years, there has been an increasing interest in the design of biomaterials for cartilage tissue engineering. This type of materials must meet several requirements. In this study, we apply ultrasound to prepare a compatibilized blend of polyelectrolyte complexes (PEC) based on carboxymethyl cellulose (CMC) and chitosan (CHI), in order to improve stability and mechanical properties through the interpolymer macroradicals coupling produced by sonochemical reaction. We study the kinetic of the sonochemical degradation of each component in order to optimize the experimental conditions for PEC compatibilization. Scaffolds obtained applying this methodology and scaffolds without ultrasound processing were prepared and their morphology (by scanning electron microscopy), polyelectrolyte interactions (by FTIR), stability and mechanical properties were analyzed. The swelling kinetics was studied and interpreted based on the structural differences between the two kinds of scaffolds. In addition we evaluate the possible in vitro cytotoxicity of the scaffolds using macrophage cells in culture. Our results demonstrate that the ultrasound is a very efficient methodology to compatibilize PEC, exhibiting improved properties compared with the simple mixture of the two polysaccharides. The test with murine macrophage RAW 264.7 cells showed no evince of cytotoxicity, suggesting that PEC biomaterials obtained under ultrasound conditions could be useful in the cartilage tissue engineering field.

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“…CNF produced with chemical pre-treatments are nanoobjects with widths less than 20 nm and lengths in the micrometer scale (Chinga-Carrasco, 2011), with advantages such as maintaining a moist environment, being strong and forming translucent structures. CNF has thus attracted much attention as a biomaterial for biomedical applications (Klemm et al, 2011;Kollar et al, 2011;Lin & Dufresne, 2014). However, in order to use CNF in contact with the human body, ultrapure CNF qualities are required, with low levels of endotoxins such as bacterially derived lipopolysaccharides (LPS).…”

Section: Introductionmentioning

confidence: 99%

Producing ultrapure wood cellulose nanofibrils and evaluating the cytotoxicity using human skin cells

Nordli

Chinga-Carrasco²,

Rokstad

et al. 2016

Carbohydrate Polymers

103

125

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 Cellulose nanofibril dispersion (50 µg/ml) did not affect the cytotoxicity or metabolic activity of Normal Human Dermal Fibroblasts and Human Epidermal Keratinocytes  Aerogels made of cellulose nanofibrils induced a reduction of metabolic activity by the fibroblasts and keratinocytes, but no significant cell death  Cytokine profiling measuring 27 cytokines revealed that the keratinocytes and fibroblasts did not induce cytokines upon direct exposure to the CNF materials. Due to the nano dimension of the CNFs, the aerogels had a high moisture-holding capacity (~7500%) 3 AbstractWood cellulose nanofibrils have been suggested as a potential wound healing material, but its utilization is limited by FDA requirements regarding endotoxin levels. In this study a method using sodium hydroxide followed by TEMPO mediated oxidation was developed to produce ultrapure cellulose nanofibrils (CNF), with an endotoxin level of 45 endotoxin units/g (EU/g) cellulose. Scanning transmission electron microscopy (S(T)EM) revealed a highly nanofibrillated structure (lateral width of 3.7±1.3 nm).Assessment of cytotoxicity and metabolic activity on Normal Human Dermal Fibroblasts and Human Epidermal Keratinocytes was done. CNF-dispersion of 50 g/ml did not affect the cells. CNF-aerogels induced a reduction of metabolic activity by the fibroblasts and keratinocytes, but no significant cell death. Cytokine profiling revealed no induction of the 27 cytokines tested upon exposure to CNF. The moisture-holding capacity of aerogels was relatively high (~7500%), compared to a commercially available wound dressing (~2500%),indicating that the CNF material is promising as dressing material for management of wounds with a moderate to high amount of exudate.

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Cytotoxicity and effects on inflammatory response of modified types of cellulose in macrophage-like THP-1 cells

Cited by 46 publications

References 19 publications

Cytocompatibility and immunomodulatory properties of wood based nanofibrillated cellulose

Cytocompatibility and immunomodulatory properties of wood based nanofibrillated cellulose

Ultrasonic compatibilization of polyelectrolyte complex based on polysaccharides for biomedical applications

Producing ultrapure wood cellulose nanofibrils and evaluating the cytotoxicity using human skin cells

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