Cytotoxicity and migration of fibroblasts on two types of calcium sulfate dihydrate

Abstract

MCS was less cytotoxic and provided greater migration of L929 fibroblasts compared with CCS.

Customized Design 3D Printed PLGA/Calcium Sulfate Scaffold Enhances Mechanical and Biological Properties for Bone Regeneration

“…When calcium levels exceed this range, cell migration is inhibited ( Arun Kumar et al, 2016 ; Aquino-Martínez et al, 2017 ). What’s more, excessive calcium ions will affect cell proliferation, and cell migration will also be affected when the number of cell proliferation is reduced ( Teparat-Burana et al, 2015 ), which was consistent with the results of in vitro biocompatibility. The PLGA/20%CaSO 4 scaffold had the highest cell viability, while the cell viability in PLGA/30%CaSO 4 decreased compared with 20%.…”

“…However, PLGA/30%CaSO 4 scaffolds had the lowest cell viability rate. This might be because Ca 2+ was the second messenger in cells, and excessive Ca 2+ affects cell signaling, thereby inhibiting cell proliferation and migration ( Teparat-Burana et al, 2015 ). We further evaluated the biocompatibility by live/dead staining assay ( Figure 5A ).…”

“…Calcium sulfate is the most commonly used bone repair material in clinical, which can cause changes in local calcium ions concentration after implantation, thereby regulating the process of bone tissue regeneration. As an intracellular second messenger and an important signaling molecule, Ca 2+ controls many key processes in cells, including proliferation, migration, and differentiation ( Teparat-Burana et al, 2015 ). Ca 2+ is released during CaSO 4 degradation Ca 2+ can bind to calmodulin (CaM) to Ca 2+ -CaM form complexes and promote osteoblast differentiation and matrix mineralization through corresponding signaling pathways ( Cao et al, 2022 ).…”