Cytotoxicity and Molecular Alterations Induced by Scorpion Venom Antimicrobial Peptide Smp43 in Breast Cancer Cell Lines MDA-MB-231 and MCF-7

Teleb, Wafaa K.; Tantawy, Mohamed A.; Xu, Xueqing; Hussein, Aida A.; Abdel-Rahman, Mohamed A.

doi:10.1007/s10989-022-10474-2

Cited by 6 publications

(2 citation statements)

References 72 publications

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“…Antitumor drugs can cause cell cycle arrest and death via activating signalling pathways 85 , 86 . Cell growth in various cell cycle phases is measured by flow cytometry 87 , 88 . The most potent compounds, 10c and 10d , were picked for additional investigation to discover how they could affect cell cycle progression in the NSC lung cancer cells, HOP-92 ( Figure 8(A,D) ).…”

Section: Resultsmentioning

confidence: 99%

Recruitment of hexahydroquinoline as anticancer scaffold targeting inhibition of wild and mutants EGFR (EGFR ^WT , EGFR ^T790M , and EGFR ^L858R )

Abo Al-Hamd,

Tawfik,

Abdullah

et al. 2023

Journal of Enzyme Inhibition and Medicinal Chemistry

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Hexahydroquinoline (HHQ) scaffold was constructed and recruited for development of new series of anticancer agents. Thirty-two new compounds were synthesised where x-ray crystallography was performed to confirm enantiomerism. Thirteen compounds showed moderate to good activity against NCI 60 cancer cell lines, with GI % mean up to 74% for 10c . Expending erlotinib as a reference drug, target compounds were verified for their inhibiting activities against EGFR WT , EGFR T790M , and EGFR L858R where compound 10d was the best inhibitor with IC 50 = 0.097, 0.280, and 0.051 µM, respectively, compared to erlotinib (IC 50 = 0.082 µM, 0.342 µM, and 0.055 µM, respectively). Safety profile was validated using normal human lung (IMR-90) cells. 10c and 10d disrupted cell cycle at pre-G1 and G2/M phases in lung cancer, HOP-92, and cell line. Molecular docking study was achieved to understand the potential binding interactions and affinities in the active sites of three versions of EGFRs.

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Section: Resultsmentioning

confidence: 99%

Recruitment of hexahydroquinoline as anticancer scaffold targeting inhibition of wild and mutants EGFR (EGFR ^WT , EGFR ^T790M , and EGFR ^L858R )

Abo Al-Hamd,

Tawfik,

Abdullah

et al. 2023

Journal of Enzyme Inhibition and Medicinal Chemistry

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“…(Panja et al 2021) Furthermore, the scorpion venom antimicrobial peptide Smp43 was discovered to be essential for the regulation of in vitro breast cancer cell survival, proliferation, cell cycle progression, apoptosis, migration, metastasis, and invasion. The results may inspire new ways of thinking about how to search for and test potential therapeutic methods and targets for the treatment of breast cancer (Teleb et al 2022).…”

Section: Discussionmentioning

confidence: 99%

Scorpion Venom Peptide Smp24 Revealed Apoptotic and Antiangiogenic Activities in Solid-Ehrlich Carcinoma Bearing Mice

Fawzy

Nafie²,

Ali³

et al. 2023

Int J Pept Res Ther

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Scorpion venom contains various peptides that could be utilized to treat various diseases, including cancer. This study aimed to evaluate the anti-cancer activity of scorpion venom peptide (Smp24) using a solid Ehrlich Carcinoma (SEC) mice model. SEC model was established by subcutaneous transplantation of SEC cells into Swiss albino female mice afterward subcutaneous injection of the Smp24 peptide compared to 5-Fluorouracil (5-FU) as a standard drug. Various biochemical, hematological, histopathological, immunohistochemical, and molecular (western blotting and RT-PCR) assays were performed to evaluate the antitumor activity of Smp24. Results revealed that Smp24 peptide significantly reduced tumor volume. Interestingly, Smp24 peptide significantly restored normal body functions in cancer-treated groups by maintaining HB, RBC’s, and WBC’s levels, reducing the elevated serum ALT and AST, and increasing total protein and albumin as well as enhancing antioxidant status through reducing the level of MDA and NO and elevating GSH, SOD, and CAT levels. Moreover, it restored the normal morphology of the liver and kidney tissues and improved hematological parameters in cancer-treated animals. Smp24 induced apoptosis in SEC cells, through upregulation of caspase-3 and BAX and the downregulation of VEGF, Bcl-2, p53, PCNA, and Ki67. Moreover, results exhibited the apoptotic and antiangiogenic effects of Smp24 against SEC cancer cells. These findings supported our previous results about the anti-cancer efficacy of Smp24 and made it a good candidate for developing effective and safe anti-cancer agents.

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F1 fraction isolated from Mesobuthus eupeus scorpion venom induces macrophage polarization toward M1 phenotype and exerts anti-tumoral effects on the CT26 tumor cell line

Sadeghi,

Amari,

Asadirad

et al. 2024

International Immunopharmacology

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Cytotoxicity and Molecular Alterations Induced by Scorpion Venom Antimicrobial Peptide Smp43 in Breast Cancer Cell Lines MDA-MB-231 and MCF-7

Cited by 6 publications

References 72 publications

Recruitment of hexahydroquinoline as anticancer scaffold targeting inhibition of wild and mutants EGFR (EGFR ^WT , EGFR ^T790M , and EGFR ^L858R )

Recruitment of hexahydroquinoline as anticancer scaffold targeting inhibition of wild and mutants EGFR (EGFR ^WT , EGFR ^T790M , and EGFR ^L858R )

Scorpion Venom Peptide Smp24 Revealed Apoptotic and Antiangiogenic Activities in Solid-Ehrlich Carcinoma Bearing Mice

F1 fraction isolated from Mesobuthus eupeus scorpion venom induces macrophage polarization toward M1 phenotype and exerts anti-tumoral effects on the CT26 tumor cell line

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Cytotoxicity and Molecular Alterations Induced by Scorpion Venom Antimicrobial Peptide Smp43 in Breast Cancer Cell Lines MDA-MB-231 and MCF-7

Cited by 6 publications

References 72 publications

Recruitment of hexahydroquinoline as anticancer scaffold targeting inhibition of wild and mutants EGFR (EGFR WT , EGFR T790M , and EGFR L858R )

Recruitment of hexahydroquinoline as anticancer scaffold targeting inhibition of wild and mutants EGFR (EGFR WT , EGFR T790M , and EGFR L858R )

Scorpion Venom Peptide Smp24 Revealed Apoptotic and Antiangiogenic Activities in Solid-Ehrlich Carcinoma Bearing Mice

F1 fraction isolated from Mesobuthus eupeus scorpion venom induces macrophage polarization toward M1 phenotype and exerts anti-tumoral effects on the CT26 tumor cell line

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Recruitment of hexahydroquinoline as anticancer scaffold targeting inhibition of wild and mutants EGFR (EGFR ^WT , EGFR ^T790M , and EGFR ^L858R )

Recruitment of hexahydroquinoline as anticancer scaffold targeting inhibition of wild and mutants EGFR (EGFR ^WT , EGFR ^T790M , and EGFR ^L858R )