Cytotoxicity Assay of Water, Ethanol N-Hexane Extract of Dates Fruit (Phoenix dactylifera) Against Murine Leukemia Cancer Cell P388

Abstract

The World Health Organization (WHO) states, in 2015, there are an estimated 9 million people who die of cancer and in 2030 there are an estimated 11.4 million deaths from cancer. Blood cancer reaches 3% of all cancers found in humans and is most common in children. The cancer drug that is available today has quite severe side effects, therefore in this study an antileukemia activity test will be carried out on water, 70% ethanol, and n-hexane extract of dates (Phoenix dactylifera). This study, the extracts from dates will be tested for cytotoxicity on Murine Leukemia P388 cells using the MTT Assay method. The IC50 value of extract of dates is used as an assessment of the activity of palm fruit extract as antileukemia. Citotoxicity assay against Murine Leukemia P388 cells by MTT assay, showed IC50 value is more than 100 µg/mL for all extracts. This result informed that the water, ethanol, and n-hexane extract of dates fruit are less potencial as a antileukemia agent.

Antioxidant and Anticancer Assessment and Phytochemical Investigation of Three Varieties of Date Fruits

“…According to the WHO, one of the main causes of death all over the world after cardiovascular diseases is cancer [ 17 ]. Among cancers, lung, breast, stomach, liver, and colorectal cancers are the leading causes of death yearly [ 17 , 18 ].…”

“…According to the WHO, one of the main causes of death all over the world after cardiovascular diseases is cancer [ 17 ]. Among cancers, lung, breast, stomach, liver, and colorectal cancers are the leading causes of death yearly [ 17 , 18 ]. Natural compounds originating from plants are one of the main sources of potential anticancer drugs [ 19 ] due to their novel structures.…”