Cytotoxicity assessment of MDA-MB-231 breast cancer cells on screen-printed graphene-carbon paste substrate

Waiwijit, Uraiwan; Kandhavivorn, W.; Oonkhanond, Bovornlak; Lomas, Tanom; Phokaratkul, D.; Wisitsoraat, Anurat; Tuantranont, Adisorn

doi:10.1016/j.colsurfb.2013.09.008

Cited by 23 publications

(13 citation statements)

References 30 publications

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“…ROS act as second messengers in many intracellular signalling cascades and lead to cellular macromolecular damage, such as membrane lipid breakdown, DNA fragmentation, protein denaturation and mitochondrial dysfunction, which greatly influence cell metabolism and signalling [175–177]. The interactions of GO with cells can lead to excessive ROS generation, which is the first step in the mechanisms of carcinogenesis, ageing, and mutagenesis [83, 122].…”

Section: Possible Toxicity Mechanisms Of Gfnsmentioning

confidence: 99%

Toxicity of graphene-family nanoparticles: a general review of the origins and mechanisms

et al. 2016

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Due to their unique physicochemical properties, graphene-family nanomaterials (GFNs) are widely used in many fields, especially in biomedical applications. Currently, many studies have investigated the biocompatibility and toxicity of GFNs in vivo and in intro. Generally, GFNs may exert different degrees of toxicity in animals or cell models by following with different administration routes and penetrating through physiological barriers, subsequently being distributed in tissues or located in cells, eventually being excreted out of the bodies. This review collects studies on the toxic effects of GFNs in several organs and cell models. We also point out that various factors determine the toxicity of GFNs including the lateral size, surface structure, functionalization, charge, impurities, aggregations, and corona effect ect. In addition, several typical mechanisms underlying GFN toxicity have been revealed, for instance, physical destruction, oxidative stress, DNA damage, inflammatory response, apoptosis, autophagy, and necrosis. In these mechanisms, (toll-like receptors-) TLR-, transforming growth factor β- (TGF-β-) and tumor necrosis factor-alpha (TNF-α) dependent-pathways are involved in the signalling pathway network, and oxidative stress plays a crucial role in these pathways. In this review, we summarize the available information on regulating factors and the mechanisms of GFNs toxicity, and propose some challenges and suggestions for further investigations of GFNs, with the aim of completing the toxicology mechanisms, and providing suggestions to improve the biological safety of GFNs and facilitate their wide application.

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Section: Possible Toxicity Mechanisms Of Gfnsmentioning

confidence: 99%

Toxicity of graphene-family nanoparticles: a general review of the origins and mechanisms

et al. 2016

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“…78 ROS act as second messengers in a variety of signaling pathways involved in apoptosis by inducing cellular macromolecular damage-such as membrane lipid breakdown, DNA fragmentation, protein denaturation, and mitochondrial dysfunction-increase in the level of oxidants, and decrease in the level of antioxidants. 34,47,79 The loss of mitochondrial membrane potential (MMP) is an early event of apoptosis. In order to determine if ROS induced mitochondrial dysfunction, we measured MMP using JC-1 monomer assay.…”

Section: Rgo-ag and Tsa Cause Mitochondrial Dysfunctionmentioning

confidence: 99%

“…ROS is the major factor inducing apoptosis by various mechanisms of macromolecular damage, such as lipid peroxidation, DNA fragmentation, protein denaturation, and mitochondrial dysfunction. 34,79,92 Graphene and graphenerelated nanoparticles possess significant genotoxic properties due to their small size, high surface area, and high surface charge. A previous study suggested that HDAC inhibition produces an increase in ROS and that this could contribute to the promotion of DNA damage.…”

Section: Rgo-ag and Tsa Potentially Induce Apoptosismentioning

confidence: 99%

Novel biomolecule lycopene-reduced graphene oxide-silver nanoparticle enhances apoptotic potential of trichostatin A in human ovarian cancer cells (SKOV3)

Zhang¹,

Huang²,

Zhang³

et al. 2017

IJN

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Background Recently, there has been much interest in the field of nanomedicine to improve prevention, diagnosis, and treatment. Combination therapy seems to be most effective when two different molecules that work by different mechanisms are combined at low dose, thereby decreasing the possibility of drug resistance and occurrence of unbearable side effects. Based on this consideration, the study was designed to investigate the combination effect of reduced graphene oxide-silver nanoparticles (rGO-AgNPs) and trichostatin A (TSA) in human ovarian cancer cells (SKOV3). Methods The rGO-AgNPs were synthesized using a biomolecule called lycopene, and the resultant product was characterized by various analytical techniques. The combination effect of rGO-Ag and TSA was investigated in SKOV3 cells using various cellular assays such as cell viability, cytotoxicity, and immunofluorescence analysis. Results AgNPs were uniformly distributed on the surface of graphene sheet with an average size between 10 and 50 nm. rGO-Ag and TSA were found to inhibit cell viability in a dose-dependent manner. The combination of rGO-Ag and TSA at low concentration showed a significant effect on cell viability, and increased cytotoxicity by increasing the level of malondialdehyde and decreasing the level of glutathione, and also causing mitochondrial dysfunction. Furthermore, the combination of rGO-Ag and TSA had a more pronounced effect on DNA fragmentation and double-strand breaks, and eventually induced apoptosis. Conclusion This study is the first to report that the combination of rGO-Ag and TSA can cause potential cytotoxicity and also induce significantly greater cell death compared to either rGO-Ag alone or TSA alone in SKOV3 cells by various mechanisms including reactive oxygen species generation, mitochondrial dysfunction, and DNA damage. Therefore, this combination chemotherapy could be possibly used in advanced cancers that are not suitable for radiation therapy or surgical treatment and facilitate overcoming tumor resistance and disease progression.

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“…In this context, considering the enormous biological applications of graphene-based nanomaterials, we demonstrated the angiogenic properties of GO and rGO, supported by several in vitro and in vivo assays [6]. The idea was initially reported by the published literature that demonstrates the killing of cancer cells by GO and rGO through formation of excessive ROS [19,20]. It is also well established that reactive oxygen species (ROS) can work as pro-angiogenic as well as anti-angiogenic agent depending upon its concentration.…”

Section: Graphene Oxides For Angiogenesis and Its Importancementioning

confidence: 95%

Graphene Oxides and the Angiogenic Process

Patra

2015

Nanomedicine (Lond.)

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Cytotoxicity assessment of MDA-MB-231 breast cancer cells on screen-printed graphene-carbon paste substrate

Cited by 23 publications

References 30 publications

Toxicity of graphene-family nanoparticles: a general review of the origins and mechanisms

Toxicity of graphene-family nanoparticles: a general review of the origins and mechanisms

Novel biomolecule lycopene-reduced graphene oxide-silver nanoparticle enhances apoptotic potential of trichostatin A in human ovarian cancer cells (SKOV3)

Graphene Oxides and the Angiogenic Process

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