Cytotoxicity evaluation and hepatoprotective potential of bioassay guided fractions from Feronia limmonia Linn leaf

Jain, Mahendra; Kapadia, Rakhee; Jadeja, Ravirajsinh N.; Thounaojam, Menaka C.; Devkar, Ranjitsinh; Mishra, S.H.

doi:10.1016/s2221-1691(11)60097-x

Cited by 37 publications

(8 citation statements)

References 13 publications

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“…HepG2 cells were treated with 1% CCl 4 either in the absence or presence of FRB‐7, marmesin or silymarin at a concentration of 10, 20, 50, 100 or 200 µg/ml for 24 h. Later, supernatants were removed and activity levels of aspartate aminotransaminase (AST) and alanine aminotransaminase (ALT) were determined. The 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide, a yellow tetrazole assay was performed in the adherent cells as per the modified method described previously [10] …”

Section: Methodsmentioning

confidence: 99%

“…Phytochemical analysis has found root bark of F. limonia to be rich in coumarins (xanthotoxin, osthenol, osthol, auraptan, isopimpinellin), tetranortriterpenes (obacunone, acidissimin), steroids (stigmasterol) and alkaloids (integriquinolone) [6–9] . Previous studies from our laboratory as well as from other research groups have shown F. limonia leaves and fruits to have hepatoprotective potential [10–13] …”

Section: Introductionmentioning

confidence: 91%

“…The 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a yellow tetrazole assay was performed in the adherent cells as per the modified method described previously. [10] Acridine orange/ethidium bromide assay HepG2 cells (5.0 ¥ 10 5 cells per well) were maintained as described earlier for 24 h and the cells were collected using a cell scraper. One microlitre of dye mixture (1 mg/ml acridine orange and 1 mg/ml ethidium bromide in phosphate buffered saline) was mixed with 9 ml cell suspension (0.5 ¥ 10 6 cells/ml) on a clean microscope slide.…”

Section: Ccl4-induced Hepatotoxicity Assay In Hepg2 Cellsmentioning

confidence: 99%

“…[6][7][8][9] Previous studies from our laboratory as well as from other research groups have shown F. limonia leaves and fruits to have hepatoprotective potential. [10][11][12][13] However, the hepatoprotective potential of F. limonia root bark extract has never been reported. Owing to this lacuna of scientific information, this study was designed to investigate the hepatoprotective potential of a methanolic extract of the root bark of F. limonia.…”

Section: Introductionmentioning

confidence: 99%

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Hepatoprotective activity of Feronia limonia root

Jain

Kapadia

Jadeja

et al. 2012

Journal of Pharmacy and Pharmacology

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 91%

Section: Ccl4-induced Hepatotoxicity Assay In Hepg2 Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hepatoprotective activity of Feronia limonia root

Jain

Kapadia

Jadeja

et al. 2012

Journal of Pharmacy and Pharmacology

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“…However, the metabolism of toxic chemicals, drugs, and virus infections in liver results in hepatic damage, gross cellular changes, and cell death causing hepatotoxicity or liver damage (Jain et al, 2011). Hepatitis is the leading cause of acute liver failure (Wang et al, 2009), and hepatic problems are responsible for a significant numbers of liver transplantations and deaths (Samal and Dangi, 2014).…”

mentioning

confidence: 99%

Hepatoprotective effect of Bifidobacterium adolescentis SPM0212 on carbon tetrachloride induced hepatotoxicity

Lee¹,

Park²,

Kim³

et al. 2015

The Korean Journal of Microbiology

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Probiotics are microbial food supplements or components of bacteria which have traditionally been added to dairy foods for extra health boost. Our aim was to evaluate the hepatoprotective effect of Bifidobacterium adolescentis SPM0212 as probiotics, which we previously found has potential anti-hepatitis B virus activity. The study was conducted using Wistar albino rats and probiotics were treated orally for 9 days consecutively and acute liver injury was induced by administration of carbon tetrachloride (CCl4) on the 7th and 8th days. Liver damage was assessed by quantifying serum activities of glutamate oxaloacetate transaminase (SGOT) and glutamate pyruvate transaminase (SGPT), as well as by histopathological examination. B. adolescentis SPM0212 significantly prevented the elevation of SGOT and SGPT levels, and reduced the negative effect of CCl4 on body and organ weights. Histopathological study revealed the livers of the carbon tetrachloride treated rats showed almost complete loss of normal hepatocyte architecture, but that rats treated with B. adolescentis SPM0212 showed minimal damage and normal hepatocyte architecture. Our results suggest that B. adolescentis SPM0212 be considered useful probiotics for protecting the liver from xenobiotics and hepatitis B virus, and as well as useful as a functional food for maintaining human health.

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Antipyretic and hepatoprotective potential of Tinospora crispa and investigation of possible lead compounds through in silico approaches

Rakib

Ahmed

et al. 2019

Food Science & Nutrition

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This research describes an investigation of the antipyretic and hepatoprotectiveproperties of both a crude organic extract and various subfractions of the ethnomedicinal plant Tinospora crispa, using appropriate animal models. In an attempt to identify potential lead hepatoprotective compounds, in silico experiments were utilized.Antipyretic activity was assessed via the Brewer's yeast-induced pyrexia method, while hepatoprotective effects were evaluated in a carbon tetrachloride (CCl 4 )induced animal model. A computer-aided prediction of activity spectra for substances (PASS) model was applied to a selection of documented phytoconstituents, with the aim of identifying those compounds with most promising hepatoprotective effects.Results were analyzed using Molinspiration software. Our results showed that both the methanol extract (METC) and various subfractions (pet ether, PEFTC; n-hexane, NHFTC; and chloroform, CFTC) significantly (p < .05) reduced pyrexia in a dose-dependent manner. In CCl 4 -induced hepatotoxicity studies, METC ameliorated elevated hepatic markers including serum alanine amino transferase (ALT), aspartate amino transferase (AST), alkaline phosphatase (ALP), and total bilirubin. Malondialdehyde (MDA) levels were significantly reduced, while superoxide dismutase (SOD) levels were significantly increased. Among a selection of metabolites of T. crispa, genkwanin was found to be the most potent hepatoprotective constituent using PASS predictive models. These results demonstrate that both the methanolic extract of T. crispa and those fractions containing genkwanin may offer promise in reducing pyrexia and as a source of potential hepatoprotective agents. K E Y W O R D S antipyretic, CCl 4 -induced hepatotoxicity, hepatoprotective, in silico studies, PASS prediction, Tinospora crispa, yeast-induced pyrexia How to cite this article: Rakib A, Ahmed S, Islam MA, et al. Antipyretic and hepatoprotective potential of Tinospora crispa and investigation of possible lead compounds through in silico approaches. Food Sci Nutr. 2020;8:547-556. https ://doi.

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Cytotoxicity evaluation and hepatoprotective potential of bioassay guided fractions from Feronia limmonia Linn leaf

Cited by 37 publications

References 13 publications

Hepatoprotective activity of Feronia limonia root

Hepatoprotective activity of Feronia limonia root

Hepatoprotective effect of Bifidobacterium adolescentis SPM0212 on carbon tetrachloride induced hepatotoxicity

Antipyretic and hepatoprotective potential of Tinospora crispa and investigation of possible lead compounds through in silico approaches

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