Cytotoxicity in L929 fibroblasts and inhibition of herpes simplex virus type 1 Kupka by estuarine cyanobacteria extracts

Abstract

The cyanobacteria are known to be a rich source of metabolites with a variety of biological activities in different biological systems. In the present work, the bioactivity of aqueous and organic (methanolic and hexane) crude extracts of cyanobacteria isolated from estuarine ecosystems was studied using different bioassays. The assessment of DNA damage on the SOS gene repair region of mutant PQ37 strain of Escherichia coli was performed. Antiviral activity was evaluated against influenza virus, HRV-2, CVB3and HSV-1 viruses using crystal violet dye uptake on HeLa, MDCK and GMK cell lines. Cytotoxicity evaluation was performed with L929 fibroblasts by MTT assay. Of a total of 18 cyanobacterial isolates studied, only the crude methanolic extract of LEGE 06078 proved to be genotoxic (IF > 1.5) in a dosedependent manner and other four were putative candidates to induce DNA damage. Furthermore, the crude aqueous extract of LEGE 07085 showed anti-herpes type 1 activity (IC50 = 174.10 μg dry extract mL −1 )while not presenting any cytotoxic activity against GMK cell lines. Of the 54 cyanobacterial extracts tested, only the crude methanolic and hexane ones showed impair on metabolic activity of L929 fibroblasts after long exposure (48-72 h). The inhibition of HSV-1 and the strong cytotoxicity against L929 cells observed emphasizes the importance of evaluating the impact of those estuarine cyanobacteria on aquatic ecosystem and on human health. The data also point out their potential application in HSV-1 treatment and pharmacological interest.

Picocyanobacteria From a Clade of MarineCyanobiumRevealed Bioactive Potential Against Microalgae, Bacteria, and Marine Invertebrates

“…To better visualize the bioactivity of extracts from diverse picocyanobacteria, a summary next to the phylogenetic tree is provided (Figure 1), with bioassay results obtained in this study compared to those from previous studies (Frazao et al, 2010;Semary and Naby, 2010;Lopes et al, 2011).The same bioactivity pattern was observed for isolates used in our study. Nevertheless, unlike that observed for other LEGE strains that represent closely related phylotypes, no apparent bioactivity was detected for the A. salina assay.…”

“…The biological activity displayed by marine and estuarine picocyanobacteria strains has been previously studied in our lab (Martins et al, 2007(Martins et al, , 2008Frazao et al, 2010;Lopes et al, 2011;Costa et al, 2014) and by other investigators (Hamilton et al, 2014). Since bioactivity effects were predominantly reported for strains of the Synechocystis and Synechococcus genera, the aim of this study was to assess the potential of novel marine Cyanobium strains as producers of bioactive compounds.…”

Pholiota nameko Polysaccharides Promotes Cell Proliferation and Migration and Reduces ROS Content in H2O2-Induced L929 Cells

“…In wound healing, a major concern is the positive response of fibroblasts toward the materials of interest; L929 is widely used for testing if the samples possess the stimulation activity by studying its cell proliferation activity [48,49]. The MTT assay was used to determine the proliferation of L929 fibroblast cells.…”

Exploring Bioactive Properties of Marine Cyanobacteria Isolated from the Portuguese Coast: High Potential as a Source of Anticancer Compounds

“…Some strains of the genera included in this study, namely Cyanobium , Synechocystis , Synechococcus and Leptolyngbya , were already identified as a potential source of bioactive compounds, based on screenings with mammals [36], invertebrates [37,38] virus, bacteria and some cell lines [39,40], although only compounds from Leptolyngbya were yet isolated [12,24]. The results obtained in the present study allow us to confirm the interest of this picoplanktonic and filamentous genera as a source of anticancer compounds.…”