Cytotoxicity mechanisms in melanoma cells and UPLC-QTOF/MS2 chemical characterization of two Brazilian stingless bee propolis: Uncommon presence of piperidinic alkaloids

Cisilotto, Júlia; Sandjo, Louis P.; Faqueti, Larissa; Fernandes, Heloísa; Joppi, Débora; Biavatti, Maique W.; Creczynski-Pasa, Tânia Beatriz

doi:10.1016/j.jpba.2017.11.038

Cited by 44 publications

(52 citation statements)

References 29 publications

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“…The antiproliferative properties of propolis from some parts of the world (Chilean, Turkish, Brazil, etc.) have been reported, however much is needed regarding their molecular mechanisms of action 18‐20 . Recently Zingue et al 21 reported the antiproliferative effects of the ethanol extract of Cameroonian propolis in the prostate but our survey revealed there has been no report on its effect against breast cancer yet.…”

Section: Introductionmentioning

confidence: 74%

The antimammary tumor effects of ethanolic extract of propolis from Adamawa region (Cameroon) are by apoptosis via reactive oxygen species‐mediated mitochondrial pathway

Zingue

Cisilotto

Fogang

et al. 2021

Environmental Toxicology

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Identification of novel natural treatment to combat cancer is a current need. This study was aimed at assessing the anticancer effects of ethanol‐extracted Cameroonian propolis (EEP). The antitumor effect of EPP was evaluated in vitro by measuring; cell viability, cell cycle, cell death mechanism, cell migration/invasion, reactive oxygen species (ROS), mitochondrial potential (ΔΨm), caspase activity, and apoptosis‐regulating proteins (Bcl‐2 and Bcl‐XL) in cell lines. In vivo, the effect of EEP against 7,12 dimethylbenz(a)anthracene (DMBA)‐induced breast tumorigenesis in rats was assessed. EEP was found to induce cytotoxicity against ER negative MDA‐MB‐231 breast cancer cells by activating apoptosis through ROS‐mediated mitochondrial pathway. The extract equally triggered caspase‐3 and caspase‐9, increment of ROS level, disruption of ΔΨm and down‐regulation of Bcl‐XL and Bcl‐2 proteins. Besides, EPP prevented migration and invasion activities by inhibiting MMP‐2 activity. At all doses it prevented breast tumor incidence (20% in EEP 150 mg/kg vs 70% in DMBA) as well as tumor burden. Tumor sections from EEP‐treated rats showed middle proliferation of mammary ducts with weak inflammatory responses. In summary, Cameroonian propolis exhibited antimammary tumor effects via the intrinsic pathway of apoptosis.

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Section: Introductionmentioning

confidence: 74%

The antimammary tumor effects of ethanolic extract of propolis from Adamawa region (Cameroon) are by apoptosis via reactive oxygen species‐mediated mitochondrial pathway

Zingue

Cisilotto

Fogang

et al. 2021

Environmental Toxicology

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“…Antimigratory activity of alloxanthin and crocoxanthin was determined as previously reported (Cisilotto et al 2018). A2058 cells (2 × 10 4 /well) were incubated and grown to 90% confluence in 24-well plates.…”

Section: Cell Migration Assaymentioning

confidence: 99%

Carotenoids from Rhodomonas salina Induce Apoptosis and Sensitize A2058 Melanoma Cells to Chemotherapy

Oliveira-Júnior

Nicolau

Bonnet

et al. 2020

Rev. Bras. Farmacogn.

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Melanoma is an aggressive tumor with invasive and metastatic potential, frequently exhibiting multidrug resistance mechanisms. In our continuous search for antimelanoma molecules, we have identified some effective marine compounds capable of not only inducing cell death, but also of sensitizing chemoresistant tumor cells to clinically used anticancer drugs. In this report, the cryptophyte Rhodomonas salina (Wislouch) D.R.A.Hill & R.Wetherbee, Pyrenomonadaceae, was chemically investigated in order to identify pigments efficiently inhibiting melanoma cells proliferation. All pharmacological tests were performed on A2058 cells expressing the oncogenic BRAF V600E mutation and resistant to dacarbazine treatment. Flash chromatography of R. salina ethanol extract led to purification of alloxanthin and crocoxanthin, which showed significant antiproliferative activity against A2058 cells, exhibiting IC 50 = 29 and 50 μM, respectively. These carotenoids promoted growth inhibition, decreased cell migration, and induced apoptosis and sub-G1 cells accumulation after 72 h of treatment. In addition, alloxanthin potentiated the cytotoxic activity of vemurafenib (a BRAF inhibitor) and restored the sensitivity of A2058 cells to dacarbazine treatment.

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“…Cell monolayers were scratched with a sterile plastic tip, washed with PBS and incubated in a new cell culture medium containing bixin (50 and 100 µM), dacarbazine (50 and 100 µM) or combined treatment, for 24h. Cell migration was microscopically (100x) monitored at 0 and 24h (Cisilotto et al, 2018). Results were expressed as percentage of cell migration calculated by measuring the cell surface using ImageJ software.…”

Section: Cell Migration Assaymentioning

confidence: 99%

Bixin, an apocarotenoid isolated from Bixa orellana L., sensitizes human melanoma cells to dacarbazine-induced apoptosis through ROS-mediated cytotoxicity

Oliveira-Júnior

Bonnet

Braconnier

et al. 2019

Food and Chemical Toxicology

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HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

show abstract

Cytotoxicity mechanisms in melanoma cells and UPLC-QTOF/MS2 chemical characterization of two Brazilian stingless bee propolis: Uncommon presence of piperidinic alkaloids

Cited by 44 publications

References 29 publications

The antimammary tumor effects of ethanolic extract of propolis from Adamawa region (Cameroon) are by apoptosis via reactive oxygen species‐mediated mitochondrial pathway

The antimammary tumor effects of ethanolic extract of propolis from Adamawa region (Cameroon) are by apoptosis via reactive oxygen species‐mediated mitochondrial pathway

Carotenoids from Rhodomonas salina Induce Apoptosis and Sensitize A2058 Melanoma Cells to Chemotherapy

Bixin, an apocarotenoid isolated from Bixa orellana L., sensitizes human melanoma cells to dacarbazine-induced apoptosis through ROS-mediated cytotoxicity

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