Cytotoxicity of AgNPs/CS composite films: AgNPs immobilized in chitosan matrix contributes a higher inhibition rate to cell proliferation

Abstract

In this study, the cytotoxicity of sliver nanoparticle-doped chitosan composite films (AgNPs/CS) was investigated in vitro. In this slow-release system, the doped silver nanoparticles (AgNPs) might modify both the surface properties of the matrix and the ion environment of the surrounding fluid via slow-release, determining the dominant mechanism is of interest. Here, AgNPs (average size is 25 nm) were doped into chitosan (CS) films by mechanical mixing to form a slow-release system. The surface properties and stabilities of the films and the Ag-releasing behavior were studied by means of X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-visible spectrophotometry, and a weight loss method. The morphology of adhered cells and the survival rate (obtained by both MTT and CCK-8 assays) of human umbilical vein endothelial cells (HUVEC) were employed to describe the cytotoxicity. Using statistical analysis, the following conclusions can be made: the doped AgNPs dispersed in the CS matrix with a polycrystalline structure. During the early erosion, a small amount of debris peeled off and became suspended in the fluid. After that erosion, the composite film became relatively stable, and the doped Ag was slowly released into the fluid. In comparison with the released Ag (either in the peeled debris or dissolved in the fluid), Ag immobilized in the AgNPs/CS films shows a more significant influence on cell adhesion and subsequent proliferation. Film thickness and AgNP content show a synergistic effect on the survival rate of the cell, with the AgNPs content being the key factor.

Silver-Chitosan Nanocomposite Prepared With Aqueous Sodium-hydroxide and Aqueous Acetic Acid Solutions: Characteristics and Their Cytotoxic Effects

“…Tyliszczak et al stated that chitosan-based hydrogels modi ed with SNPs produced by sodium borohydride in concentrations of 25, 50, 75 and 100 (wt%), showed no toxic effect on dermis cells BJ (CRL-2522TM) [9]. Wang et al reported that silver immobilized in the sliver nanoparticle-doped chitosan composite lms, shows a signi cant in uence on the cell adhesion and subsequent proliferation of human umbilical vein endothelial cells [11]. Jena et al reported that chitosan-coated silver nanoparticles, using chitosan as stabilizing and reducing agent, showed no signi cant cytotoxic or DNA damage on the macrophages at the bactericidal dose [13].…”

“…Various size of the nanoparticles, different types of cell lines affect the cytotoxicity. Accordingly, some reports showed the cytotoxicity, and anti-cancer effects of silver-chitosan nanocomposite on human umbilical artery endothelial cells (HUAECs) and A549 cells (Lung cancer cell line), respectively [10,11]. In contrast, some publications advocated the non-toxicity of the chitosan-coated silver nanoparticles on healthy dermis cells (ATCC CRL-2522TM), and macrophages [9,12,13].…”

Μελέτη Της Επίδρασης Τεχνητών Νανοσωματιδίων Αργύρου Στη Διαπερατότητα Του Υπεζωκότα Προβάτου

“…Δεν υπάρχουν παρόμοιες μελέτες σε μεσοθηλιακά κύτταρα που να εξετάζουν τις επιδράσεις των AgNPs στις δύο αυτές κυτταρικές λειτουργίες, με τις οποίες θα μπορούσαμε να συγκρίνουμε τα αποτελέσματα της παρούσας διατριβής. Σε αντίθεση με τα δικά μας ευρήματα, τα AgNPs έχει αποδειχθεί ότι αναστέλλουν την προσκόλληση και τον πολλαπλασιασμό των ανθρώπινων ενδοθηλιακών κυττάρων ομφαλικής φλέβας[121]. Υπάρχει, ωστόσο, μια θεμελιώδης διαφορά μεταξύ των δύο μελετών, καθώς τα ενδοθηλιακά κύτταρα αφέθηκαν να προσκολληθούν σε φιλμ χιτοζάνης AgNPs με μεταβλητό πάχος ενώ στην παρούσα διατριβή αφέθηκαν να προσκολληθούν σε υπόστρωμα φιμπρονεκτίνης.…”