Cytotoxicity of antibody-coated trypanosomes by normal human lymphoid cells

Mkwananzi, J. B.; Franks, D.; Baker, John

doi:10.1038/259403a0

Cited by 37 publications

(12 citation statements)

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“…SCMC is mediated by unsensitized effector cells and affects a variety of target cells in culture (Takasugi, Mickey & Terasaki 1973); it may be of importance in immune surveillance. ADCC is based on the ability of lymphoid cells from non-immune individuals to destroy antibody coated target cells specifically (Perlmann & Perlmann 1970); this mechanism may protect the host against certain fungal and parasitic infections (Mcwananzi, Franks & Baker 1976). In this investigation, an examination has been made of both SCMC and ADCC activity in the peripheral blood of a group of 25 patients with periodontal disease and 25 control subjects matched for age and sex.…”

Section: Introductionmentioning

confidence: 99%

Human antibody‐dependent cellular cytotoxicity and natural killer cytotoxicity in periodontal disease

Tsoumis

Singh

Dolby

1985

J of Periodontal Research

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Spontaneous cell mediated cytotoxicity (SCMC) and antibody‐dependent cell mediated cytotoxicity (ADCC) were examined in 25 patients with periodontal disease and a group of 25 age and sex matched control subjects. SCMC and ADCC were evaluated from the 51Cr activity released when peripheral blood lymphocytes were cultured with 51Cr labelled K562 and L929 cells, respectively. Four lymphocyte: target cell ratios were used, 50: 1, 25: 1, 12.5: 1, and 6.25: 1 in triplicate. There was no significant difference (p>0.05) in the SCMC and ADCC between the two groups. However, a very significant positive correlation (p < 0.001) was found between the degree of periodontal disease (Russell 1956) and SCMC.

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Section: Introductionmentioning

confidence: 99%

Human antibody‐dependent cellular cytotoxicity and natural killer cytotoxicity in periodontal disease

Tsoumis

Singh

Dolby

1985

J of Periodontal Research

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“…Living cultures of T. dionisii take up ["mTc]pertechnetate in the presence of unlabeled sodium chromate, and release of the radioactive isotope can be used as a measure of cytotoxicity. Using this labeling procedure, Mkwananzi et al (22) have shown that human lymphocytes are cytotoxic to antibody-coated T. dionisii. Abrahamson and da Silva (1) used irreversible loss of motility to prove that mouse spleen lymphocytes are cytotoxic to antibody-coated T. cruzi epimastigotes.…”

mentioning

confidence: 99%

Role of hydrogen peroxide and peroxidase in the cytotoxicity of Trypanosoma dionisii by human granulocytes

Thorne¹,

Svvennsen²,

Franks³

1978

Infect Immun

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The mechanism of the cytotoxic reaction of leukocytes to Trypanosoma dionisii was investigated. Cytotoxicity was measured by release of [aamTc]pertechnetate from labeled protozoa. Both granulocytes and lymphocytes were found to be cytotoxic to antibody-coated T. dionisii. The reaction was inhibited by diethyldithiocarbamate and by potassium cyanide, both of which inhibit myeloperoxidase. Myeloperoxidase from azurophil granules was toxic to T. dionisii, provided that hydrogen peroxide was also present. Hydrogen peroxide formation was induced in granulocytes and, to a lesser extent, in lymphocytes by antibodycoated T. dionisii. Inhibition of this hydrogen peroxide formation by treatment of the effector cell surface withp-diazobenzenesulfonic acid inhibited cytotoxicity. It is therefore concluded that granulocytes, and probably also lymphocytes, kill T. dionisii with hydrogen peroxide by a peroxidase-mediated reaction. Although hydrogen peroxide and myeloperoxidase alone were also cytotoxic to the lymphoblastoid cell line CLA4, it seems unlikely that this is the cytotoxic mechanism for this process because these cells were unable to induce hydrogen peroxide formation.

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“…It has been demonstrated that leukocytes can, in the presence of specific antibody, cause extracellular lysis of a variety of target cells, such as tumor cells (3,16,24,31,35), virus-infected cells (16,19,22,23), parasites (16,30), fungi (7,16,33), and bacteria (13,28,29,34). This antibodydependent cellular cytotoxicity (ADCC) occurs in the absence of complement.…”

mentioning

confidence: 99%

Cytotoxicity of human peripheral blood and colostral leukocytes against Shigella species

Morgan¹,

DuPont²,

Gonik³

et al. 1984

Infect Immun

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We examined the ability of human peripheral blood leukocytes to kill strains of Shigella sonnei and Shigella flexneri by using a modified bactericidal assay. Antibody-dependent cellular cytotoxicity (ADCC) was demonstrated in the presence of specific rabbit immune serum directed against S. sonnei. With peripheral blood leukocytes from adults, ADCC was found only in the mononuclear cell and purified lymphocyte populations. Monocyte-macrophages and polymorphonuclear leukocytes were unable to demonstrate ADCC. Lymphocyte ADCC, which was not affected by the addition of phenylbutazone (an inhibitor of phagocytosis), was mediated by a non-T, Fc receptor-positive, HNK-1-cell. ADCC (using antiserum directed against virulent S. sonnet) was demonstrated against virulent S. sonnei but not against avirulent S. sonnei or virulent S. flexneri. In contrast to leukocytes from adults, both mononuclear and polymorphonuclear cells from neonatal cord blood and from a patient with chronic granulomatous disease mediated anti-Shigella ADCC. Breast milk leukocytes (BMLs) collected 1 to 3 days postpartum were used as effector cells against virulent S. sonnei. The entire BML population, BMLs which did not adhere to plastic and BMLs which passed through nylon wool columns mediated both natural killer cytotoxicity and ADCC. In paired experiments, natural killer cytotoxicity and ADCC were significantly lower (30 to 45% inhibition) but not ablated, when phenylbutazone was added to BMLs and nylon wool-purified BMLs (P < 0.05). These experiments suggest that colostral leukocytes mediated both extracellular and intracellular bacteriolysis in the presence and absence of specific antiserum. These mechanisms may be active in vivo in protection against shigellosis.

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Cytotoxicity of antibody-coated trypanosomes by normal human lymphoid cells

Cited by 37 publications

References 2 publications

Human antibody‐dependent cellular cytotoxicity and natural killer cytotoxicity in periodontal disease

Human antibody‐dependent cellular cytotoxicity and natural killer cytotoxicity in periodontal disease

Role of hydrogen peroxide and peroxidase in the cytotoxicity of Trypanosoma dionisii by human granulocytes

Cytotoxicity of human peripheral blood and colostral leukocytes against Shigella species

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