Human antibody‐dependent cellular cytotoxicity and natural killer cytotoxicity in periodontal disease

Abstract

Spontaneous cell mediated cytotoxicity (SCMC) and antibody‐dependent cell mediated cytotoxicity (ADCC) were examined in 25 patients with periodontal disease and a group of 25 age and sex matched control subjects. SCMC and ADCC were evaluated from the 51Cr activity released when peripheral blood lymphocytes were cultured with 51Cr labelled K562 and L929 cells, respectively. Four lymphocyte: target cell ratios were used, 50: 1, 25: 1, 12.5: 1, and 6.25: 1 in triplicate. There was no significant difference (p>0.05) in the SCMC and ADCC between the two groups. However, a very significant positive correlation (p < 0.001) was found between the degree of periodontal disease (Russell 1956) and SCMC.

Cytotoxic and immunostimulatory effects of Bacteroides cell products

“…Therefore, host cellular immune responses may be considered partially responsible for the progression of alveolar bone loss. Fibroblasts derived from the periodontium have been shown to be susceptible to cytotoxic products of lymphocytes obtained from inflamed gingiva (19,(32)(33)(34). In addition, in vitro studies using the CRL-1292 gingival fibroblast line has demonstrated that BG wall fragments can induce the release of thymocyte-activating factor from murine thymocytes (21), Two methods by which suspected pathogens such as BG and Bl may stimulate cytotoxic lymphocytes include; 1) human antibody-dependent cellular cytotoxicity (ADCC), and 2) natural killer cytotoxicity (NKC), The incubation of peripheral human lymphocyte populations in autologous serum with BG sonicates resulted in significant cytotoxicity (P< 0,005) (Table 5), However, this was only shown when media supernates plus cells were added to target fibroblasts.…”

“…While chronic adult periodontal disease is viewed primarily as a plasma cell lesion, the initiation and periodic nature of this disease suggests the importance of T-cell interactions (17,18). Lymphocyte-mediated cytotoxicity may also play a role in periodontal and periapical disease through antibody dependent cellular cytotoxicity or natural killer (NK) cell activity as has been suggested by in vitro studies (19). Humoral and cellular immune components are thought to pass through tissue interfaces which allows for relatively large molecular weight products to can gain entry to the systemic circulation and be made available to serve as nonspecific or antigenic stimulators of immune effector cells.…”

Immunohistological analysis of CD1a + langerhans cells and CD57 + natural killer cells in healthy and diseased human gingival tissue: A comparative study

“…This finding is in conformity with previously published results. [72526] Tsoumis,[6] demonstrated a significant positive correlation between peripheral blood NK cytotoxicity and degree of inflammatory periodontal disease. The precise role played by these cells in modulating periodontal disease is not fully understood.…”

“…Tsoumis[6] demonstrated a significant positive correlation between peripheral blood NK cytotoxicity and degree of inflammatory periodontal disease. Fujita[7] reported an increased infiltration of the gingival connective tissue by NK cells in severe forms of periodontal disease and suggested that these cells may play a role in the destruction of tissues in periodontal disease.…”

Induction of Activated Lymphocyte Killing by Bacteria Associated with Periodontal Disease

“…Tsoumis et al (1985) demonstrated a significant positive correlation between peripheral blood NK cytotoxicity and the degree of inflammatory periodontal disease. An increase in antibody-dependent cellular cytotoxicity, mediated by NK cells, was noted in the early stages of the chronic mucosal disease, recurrent aphthous ulceration (Greenspan et al, 1981).…”