Cytotoxicity of Different Nano Composite Resins on Human Gingival and Periodontal Ligament Fibroblast Cell Lines: An In Vitro Study

Abstract

The aim of this study is to determine the cytotoxicity of three different nano composite resins (CRs) on human gingival fibroblast (hGF) and periodontal ligament fibroblast (hPDLF) cell lines. These CRs selected were nanohybrid organic monomer-based Admira Fusion (AF), nanohybrid Bis-(acryloyloxymethyl) tricyclo [5.2.1.0.sup.2,6] decane-based Charisma Topaz (CT), and supra nano filled resin-based Estelite Quick Sigma (EQS). MTT assay was performed to assess the cytotoxicity of CRs at 24 h and one week. AF and EQS applied on hGF cells at 24 h and one week demonstrated similar cytotoxic outcomes. Cytotoxicity of CT on hGF cells at one week was higher than 24 h (p = 0.04). Cytotoxicity of CT on hGF cells was higher at 24 h (p = 0.002) and one week (p = 0.009) compared to control. All composites showed higher cytotoxicity on hPDLF cells at one week than the 24 h (AF; p = 0.02, CT; p = 0.02, EQS; p = 0.04). AF and EQS demonstrated lower cytotoxicity on hPDLF cells than the control group at 24 h (AF; p = 0.01, EQS; p = 0.001). CT was found more cytotoxic on hPDLF cells than the control (p = 0.01) and EQS group (p = 0.008) at one week. The cytotoxicity of CRs on hGF and hPDLF cells vary, according to the type of composites, cell types, and exposure time.

Antimicrobial agents in dental restorative materials: Effect on polymerization, short‐term drug release and biological impact

“…However, elution of the residual monomers in water‐based systems is estimated to be rather low [59–61]. Ormocer materials do not contain any free dimethacrylate monomers [62], which is also stated by the manufacturer of the ormocer Admira Fusion [49] used here, and they therefore result in a less cytotoxic behavior on human gingival and periodontal ligament fibroblasts compared to resin‐based composites [63].…”

Considerations about Cytotoxicity of Resin-Based Composite Dental Materials: A Systematic Review

“…Enamel Plus HRi and G-aenial showed, respectively, severe and moderate toxicity after 48 h. After 72 h of incubation, Omnichroma and Omnichroma Blocker showed mild cytotoxicity with a significant decrease in cell viability rates as compared to levels after 48 h. Admira Fusion x-tra and Enamel Plus HRi Bio Function Enamel showed a significant reduction to moderate cytotoxicity after 72 h. G-aenial Flo X and Enamel Plus HRi Bio Function Bio Dentine showed similar results after 48 h and 72 h. Both materials showed a lower cell viability rate after 72 h as compared to 48 h incubation [ 39 ]. Kavuncu et al tested three composite materials, Admira Fusion, Charisma Topaz, and Estelite Quick Sigma, on human gingival fibroblasts and periodontal ligament fibroblasts [ 40 ]. In comparison to the control group, Admira Fusion, and Estelite Quick Sigma presented no cytotoxic effects on gingival fibroblasts after 24 h incubation.…”

“…After 1 week of incubation, the viability of gingival fibroblasts exposed to Charisma Topaz was significantly lower than the control, while the other materials were similar to the control. In contrast, Charisma Topaz did not produce significant cytotoxicity on periodontal ligament fibroblasts after 24 h of incubation and was the only cytotoxic material in 1 week of exposure [ 40 ]. Sulek et al tested the cytotoxicity of Charisma, Estelite Sigma Quick, and Filtek Z250 on human gingival fibroblasts via MTT assay [ 41 ].…”

Biological Response Induced in Primary Human Gingival Fibroblasts upon Exposure to Various Types of Injectable Astringent Retraction Agents

“…Moreover, they respond to different external stimuli, including mechanical stress [ 25 ]. Their presence in the oral mucosa lamina propria, as well as their multiple biological functions, made HGFs a common model for the evaluation of cytotoxicity of dental materials [ 26 , 27 ].…”