The aim of this study is to determine the cytotoxicity of three different nano composite resins (CRs) on human gingival fibroblast (hGF) and periodontal ligament fibroblast (hPDLF) cell lines. These CRs selected were nanohybrid organic monomer-based Admira Fusion (AF), nanohybrid Bis-(acryloyloxymethyl) tricyclo [5.2.1.0.sup.2,6] decane-based Charisma Topaz (CT), and supra nano filled resin-based Estelite Quick Sigma (EQS). MTT assay was performed to assess the cytotoxicity of CRs at 24 h and one week. AF and EQS applied on hGF cells at 24 h and one week demonstrated similar cytotoxic outcomes. Cytotoxicity of CT on hGF cells at one week was higher than 24 h (p = 0.04). Cytotoxicity of CT on hGF cells was higher at 24 h (p = 0.002) and one week (p = 0.009) compared to control. All composites showed higher cytotoxicity on hPDLF cells at one week than the 24 h (AF; p = 0.02, CT; p = 0.02, EQS; p = 0.04). AF and EQS demonstrated lower cytotoxicity on hPDLF cells than the control group at 24 h (AF; p = 0.01, EQS; p = 0.001). CT was found more cytotoxic on hPDLF cells than the control (p = 0.01) and EQS group (p = 0.008) at one week. The cytotoxicity of CRs on hGF and hPDLF cells vary, according to the type of composites, cell types, and exposure time.

Objective:  In this case report, both the diagnosis of pemphigus vulgaris and the periodontal treatment approach including the use of local/systemic medications are presented. Case Presentation: 36-year-old female patient applied to the periodontology clinic with complaints of burning mouth and pain. Physical examination revealed cutaneous blisters on nose, hand and fingers while intra-oral examination showed widespread desquamation and ulcers depending on PV and severe gingival inflammation due to the lack of oral hygiene and oral PV. Initial periodontal treatment (IPT) was implemented to the patient along with local and systemic medications. Periodontal parameters including plaque Index (PI), gingival Index (GI), probing depth (PD) and clinical attachment level (CAL) were recorded before and six weeks after IPT.  Periodontal treatment procedures did not cause any negative effect on the lesions. Six weeks following IPT and the use systemic medications, all clinical parameters improved significantly. Furthermore, lesions including mucosal blisters and desquamations partially recovered, the patient started to perform oral hygiene more effectively. It was concluded that atraumatic and non-invasive periodontal treatment supported by the use of local/systemic corticosteroid and immunosuppressive medications was efficient on controlling of widespread desquamations and gingival inflammation of PV patients.

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