Cytotoxicity of gold nanoparticles in human neural precursor cells and rat cerebral cortex

Lee, Uhn; Yoo, Chan-Jong; Kim, Yong-Jung; Yoo, Young-Mi

doi:10.1016/j.jbiosc.2015.07.004

Cited by 41 publications

(26 citation statements)

References 21 publications

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“…9 In recent years, the toxicity of AuNPs has been reported, which depends largely on their physicochemical properties such as shape, size and surface chemistry. 10,11 Particle size is one of the most important factors in determining the interactions of AuNPs with biological systems, ultimately influencing their uptake and toxicity. 10,12,13 AuNPs with a small particle size can pass through cells or subcellular organelles such as nucleus, which may facilitate the delivery of therapeutic molecules into cells.…”

Section: Introductionmentioning

confidence: 99%

“…10,11 Particle size is one of the most important factors in determining the interactions of AuNPs with biological systems, ultimately influencing their uptake and toxicity. 10,12,13 AuNPs with a small particle size can pass through cells or subcellular organelles such as nucleus, which may facilitate the delivery of therapeutic molecules into cells. 14 For example, Elbakry et al reported the size-dependent delivery of DNA into cells by layer-by-layer-coated AuNPs (20 nm, 30 nm, 50 nm and 80 nm).…”

Section: Introductionmentioning

confidence: 99%

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<p>Size- and cell type-dependent cellular uptake, cytotoxicity and in vivo distribution of gold nanoparticles</p>

Xia

Huang

Feng

et al. 2019

IJN

117

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Background: Gold nanoparticles (AuNPs) have shown great promise in biomedical applications. However, the interaction of AuNPs with biological systems, its underlying mechanisms and influencing factors need to be further elucidated. Purpose: The aim of this study was to systematically investigate the effects of particle size on the uptake and cytotoxicity of AuNPs in normal cells and cancer cells as well as their biological distribution in vivo. Results: Our data demonstrated that the uptake of AuNPs increased in HepG2 cancer cells but decreased in L02 normal cells, with the increase of particle size (5-50 nm). In both cancer cells and normal cells, small (5 nm) AuNPs exhibited greater cytotoxicity than large ones (20 and 50 nm). Interestingly, 5 nm AuNPs induced both apoptosis and necrosis in HepG2 cells through the production of reactive oxygen species (ROS) and the activation of pro-caspase3, whereas it mainly induced necrosis in L02 cells through the overexpression of TLR2 and the release of IL-6 and IL-1a cytokines. Among them, 50 nm AuNPs showed the longest blood circulation and highest distribution in liver and spleen, and the treatment of 5 nm AuNPs but not 20 nm and 50 nm AuNPs resulted in the increase of neutrophils and slight hepatotoxicity in mice. Conclusion: Our results indicate that the particle size of AuNPs and target cell type are critical determinants of cellular uptake, cytotoxicity and underlying mechanisms, and biological distribution in vivo, which deserves careful consideration in the future biomedical applications.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

<p>Size- and cell type-dependent cellular uptake, cytotoxicity and in vivo distribution of gold nanoparticles</p>

Xia

Huang

Feng

et al. 2019

IJN

117

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“…A great variety of new nanoparticles (NPs), with specific features have been under intense investigation [1][2][3][4][5][6][7][8]. Also, there are many studies on the effects of these nanoparticles within the human body [9][10][11][12], and a huge number of new nanodrugs have been approved for clinical use worldwide [13]. Despite all these data, and the supposed advantages of nanomedicine/nandodrugs, many aspects of the biological interaction as the human behavior are still unrevealed, especially in the molecular and cellular level.…”

Section: Introductionmentioning

confidence: 99%

Molecular and Cellular Risk Assessment of Healthy Human Cells and Cancer Human Cells Exposed to Nanoparticles

Helal-Neto

Silva

Saldanha-Gama

et al. 2019

IJMS

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Nanodrugs have in recent years been a subject of great debate. In 2017 alone, almost 50 nanodrugs were approved for clinical use worldwide. Despite the advantages related to nanodrugs/nanomedicine, there is still a lack of information regarding the biological safety, as the real behavior of these nanodrugs in the body. In order to better understand these aspects, in this study, we evaluated the effect of polylactic acid (PLA) nanoparticles (NPs) and magnetic core mesoporous silica nanoparticles (MMSN), of 1000 nm and 50 nm, respectively, on human cells. In this direction we evaluated the cell cycle, cytochemistry, proliferation and tubulogenesis on tumor cells lines: from melanoma (MV3), breast cancer (MCF-7, MDA-MB-213), glioma (U373MG), prostate (PC3), gastric (AGS) and colon adenocarcinoma (HT-29) and non-tumor cell lines: from human melanocyte (NGM), fibroblast (FGH) and endothelial (HUVEC), respectively. The data showed that an acute exposure to both, polymeric nanoparticles or MMSN, did not show any relevant toxic effects on neither tumor cells nor non-tumor cells, suggesting that although nanodrugs may present unrevealed aspects, under acute exposition to human cells they are harmless.

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“…The common method is to implant the test material into the body of an animal. However, implantation in vivo is limited by the long experimental period, complicated operation process, complex body environment and parameter control, among others (Wang et al, 2012;Lee et al, 2016). In contrast, experiments conducted in vitro are relatively simple and their reproducibility can easily be controlled (Li et al, 2015).…”

Section: Time (H) Groups Ckmentioning

confidence: 99%

Expression and functional evaluation of Mytilus galloprovincialis foot protein type 5 (Mgfp-5), the recombinant mussel adhesive protein

YaWei¹,

Zhang²,

Gao³

et al. 2017

Afr. J. Biotechnol.

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Mussel contains a variety of adhesion proteins, among which, Mytilus galloprovincialis foot protein type 5 (Mgfp-5) is one of the major proteins required for substrate adhesion. The labor-intensive nature and insufficiency of the extraction process have frequently resulted in very little purified recombinant Mgfp-5. These prompt technologies such as chemical synthesis and genetic engineering are employed to overcome these limitations. In this study, successful expression and purification of the recombinant Mgfp-5 using Escherichia coli BL21 (DE3) and affinity chromatography were reported. Production yield of 12.25% and purity of 96.92%, respectively were observed. The 3,4-dihydroxyphenylalanine (DOPA) content (9.60 pmol/g) and the adhesion (1 116 nN) in modified recombinant Mgfp-5 were 9.32 times and 1.6 times as great as those in the unmodified recombinant Mgfp-5, respectively. Recombinant Mgfp-5 at a concentration of 9.6 mg/L had little cytotoxicity on mouse L-929 fibroblast cells, which was toxic at first in cytotoxicity test, and a concentration of not more than 20 μg/mL would not lead to hemolysis of rabbit erythrocytes. In this case, recombinant Mgfp-5 is biosecure, providing the foundation for Mgfp-5 manufacturing as well as the development of clinical biological adhesive.

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Cytotoxicity of gold nanoparticles in human neural precursor cells and rat cerebral cortex

Cited by 41 publications

References 21 publications

<p>Size- and cell type-dependent cellular uptake, cytotoxicity and in vivo distribution of gold nanoparticles</p>

<p>Size- and cell type-dependent cellular uptake, cytotoxicity and in vivo distribution of gold nanoparticles</p>

Molecular and Cellular Risk Assessment of Healthy Human Cells and Cancer Human Cells Exposed to Nanoparticles

Expression and functional evaluation of Mytilus galloprovincialis foot protein type 5 (Mgfp-5), the recombinant mussel adhesive protein

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