Cytotoxicity of iron (III), molybdenum (III), and their mixtures in BALB/3T3 and HepG2 cells

Terpiłowska, Sylwia; Siwicka-Gieroba, Dorota; Siwicki, Andrzej K.

doi:10.2478/jvetres-2018-0066

Cited by 20 publications

(9 citation statements)

References 22 publications

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“…Still, the cells demonstrated a comparable response to the material, with cells spreading and dividing across the test sample, indicating a similar initial level of biocompatibility as the positive control. There are studies suggesting iron has a cytotoxic effect [56,57] but that was not confirmed in this study. This is in accordance with previous studies on the biocompatibility of silicon nitride, where in vitro studies found osteoblask-like cells to grown and proliferate on polished silicon nitride discs [29] as well as silicon carbon nitride coatings [58] and no cytotoxic effects when exposing silicon nitride material to fibroblast cells [30].…”

Section: Cell Responsecontrasting

confidence: 78%

Si–Fe–C–N Coatings for Biomedical Applications: A Combinatorial Approach

et al. 2020

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Ceramic coatings may prolong the lifetime of joint implants. Certain ions and wear debris may however lead to negative biological effects. SiN-based materials may substantially reduce these effects, but still need optimization for the application. In this study, a combinatorial deposition method enabled an efficient evaluation of a range of Si–Fe–C–N coating compositions on the same sample. The results revealed compositional gradients of Si (26.0–33.9 at.%), Fe (9.6–20.9 at.%), C (8.2–13.9 at.%) and N (39.7–47.2 at.%), and low oxygen contaminations (0.3–0.6 at.%). The mechanical properties varied with a hardness (H) ranging between 13.7–17.3 GPa and an indentation modulus (M) between 190–212 GPa. Both H and M correlated with the Si (H and M increased as Si increased) and Fe (H and M decreased as Fe increased) content. A slightly columnar morphology was observed in cross-sections, as well as a surface roughness in the nm range. A cell study revealed adhering pre-osteogenic MC3T3 cells, with a morphology similar to that of cells seeded on a tissue culture plastic control. The investigated coatings could be considered for further investigation due to the ability to tune their mechanical properties while maintaining a smooth surface, together with their promising in vitro cell response.

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Section: Cell Responsecontrasting

confidence: 78%

Si–Fe–C–N Coatings for Biomedical Applications: A Combinatorial Approach

et al. 2020

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“…The cell viability of Balb/3T3 mouse embryo fibroblasts and HaCaT human skin keratinocytes exposed to different concentrations of eADF4(κ16)-coated Se NPs and PVA coated Se NPs were measured using the alamarBlue ® assay (Figure 2). Balb/3T3 mouse embryo fibroblasts are frequently used to test materials' carcinogenicity 68 and cytotoxicity, 69,70 and HaCaT keratinocytes are a preliminary in vitro model to investigate skin toxicity. 71 Both of these cell lines have been widely used for cytotoxicity tests of nanoparticles.…”

Section: Cytotoxicity Test Of Selenium Nanoparticles Using Fibroblastmentioning

confidence: 99%

<p>Enhanced Antibacterial Activity of Se Nanoparticles Upon Coating with Recombinant Spider Silk Protein eADF4(κ16)</p>

Huang¹,

Kumari²,

Herold³

et al. 2020

IJN

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Purpose: Selenium nanoparticles (Se NPs) are promising antibacterial agents to tackle the growing problem of antimicrobial resistance. The aim of this study was to fabricate Se NPs with a net positive charge to enhance their antibacterial efficacy. Methods: Se NPs were coated with a positively charged proteinrecombinant spider silk protein eADF4(κ16)to give them a net positive surface charge. Their cytotoxicity and antibacterial activity were investigated, with negatively charged polyvinyl alcohol coated Se NPs as a control. Besides, these eADF4(κ16)-coated Se NPs were immobilized on the spider silk films, and the antibacterial activity of these films was investigated. Results: Compared to the negatively charged polyvinyl alcohol coated Se NPs, the positively charged eADF4(κ16)-coated Se NPs demonstrated a much higher bactericidal efficacy against the Gram-negative bacteria E. coli, with a minimum bactericidal concentration (MBC) approximately 50 times lower than that of negatively charged Se NPs. Cytotoxicity testing showed that the eADF4(κ16)-coated Se NPs are safe to both Balb/3T3 mouse embryo fibroblasts and HaCaT human skin keratinocytes up to 31 µg/mL, which is much higher than the MBC of these particles against E. coli (8 ± 1 µg/mL). In addition, antibacterial coatings were created by immobilising the eADF4(κ16)-coated Se NPs on positively charged spider silk films and these were shown to retain good bactericidal efficacy and overcome the issue of low particle stability in culture broth. It was found that these Se NPs needed to be released from the film surface in order to exert their antibacterial effects and this release can be regulated by the surface charge of the film, such as the change of the spider silk protein used. Conclusion: Overall, eADF4(κ16)-coated Se NPs are promising new antibacterial agents against life-threatening bacteria.

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“…It is to be noted that the essential ion calcium(II) can be cytotoxic and promote hemostasis and the aggregation of certain cells at high concentrations 68,69 . In addition, although iron is indispensable for cells, a higher concentration of its ions can induce genotoxicity and mutagenicity 70 . Thus, the developed blends were evaluated using two different methods: osteoblast culture and CAM assay.…”

Section: Resultsmentioning

confidence: 99%

Four modified sodium alginate/carboxymethylcellulose blends for prednisone delivery

Silva

Costa

Dourado

et al. 2020

J of Applied Polymer Sci

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Polysaccharides have been widely used for the development of drug delivery systems. These systems can be physicochemically modified to enhance their stabilities and control their drug release profiles. However, such modifications cannot alter their biocompatibility or toxicity. Herein, four structurally modified sodium alginate/carboxymethylcellulose blends are synthesized and loaded with prednisone, and the effects of the modifications on their hydrolytic degradation rates, biocompatibilities, toxicities, and drug release profiles are investigated. All the blends are ionically cross‐linked with Ca2+ and Fe3+. Blend 1 is not modified further, blend 2 is additionally reinforced with 8% w/w of cellulose nanocrystals, blend 3 is treated with epoxidized linseed oil to develop a hydrophobic layer, and blend 4 is chemically cross‐linked with epichlorohydrin. Blends 2 and 4 exhibit similar physicochemical characteristics, appropriate hydrolytic degradation rates and drug release patterns, as well as biocompatibility and non‐toxicity. In‐vitro studies using the osteoblasts and CAM assay demonstrate that blends 2 and 4 are also biocompatible and non‐toxic. In contrast, blend 1 exhibits the highest drug release rate, followed by blend 3.

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Cytotoxicity of iron (III), molybdenum (III), and their mixtures in BALB/3T3 and HepG2 cells

Cited by 20 publications

References 22 publications

Si–Fe–C–N Coatings for Biomedical Applications: A Combinatorial Approach

Si–Fe–C–N Coatings for Biomedical Applications: A Combinatorial Approach

<p>Enhanced Antibacterial Activity of Se Nanoparticles Upon Coating with Recombinant Spider Silk Protein eADF4(κ16)</p>

Four modified sodium alginate/carboxymethylcellulose blends for prednisone delivery

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