Cytotoxicity of Iron Nanoparticles Synthesized Using Dried Ginger

et al. 2021

JPRI

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Introduction: Nanotechnology is getting used in developing countries to assist, treat disease, and stop health issues. Nanotechnology in addition to nanomedicine is being applied to or developed for application to a spread of commercial and purification processes. Ginger, scientifically known as Zingiber officinale, belongs to the family Zingiberaceae. Aim: The aim of the study was to green synthesis copper nanoparticles using ginger oleoresin and to evaluate its cytotoxic activity against liver cancer cells. Materials and Methods: The Ginger oleoresin was obtained from Synthite Industries Private Limited, Kerala with a product code: 4010000370 was used for the study. Copper nanoparticles were prepared from oleoresin and confirmed using UV-Visible spectroscopy. The prepared copper nanoparticles were then evaluated for the anticancer effect on liver cancer cells using the method of Mosmann. The cells (1 × 105 cells per ml) were seeded in a 96 well microtiter plate (100 μl per well) with replications. Different concentration (control, 10, 20, 30, 40, and 60 μg/ml) of ginger oleoresins mediated copper nanoparticles were tested for the anticancer activity. The percentage of cell viability was calculated. Results: Dose-dependent anticancer activity was observed with ginger oleoresins mediated copper Nanoparticles. The IC 50 value was 30 mg / ml Conclusion: In the present study, ginger oleoresin showed a good activity cytotoxic effect on liver cancer cell lines. However, more research is needed to understand the underlying mechanisms of the cytotoxic property of the ginger plant.

Section: Discussionsupporting

confidence: 70%

Green Synthesis of Copper Nanoparticles Using Ginger Oleoresin and Evaluation of its Anticancer Activity against Liver Cancer Cell Line

Kumar

Anitha

et al. 2021

JPRI

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“…Initially the iron nanoparticles were identified by stable dark brown colour and the surface plasmon resonance was at the peak positioned at 370 nm. Their study supported that dried ginger Zingiber are important sources for potent biologic activities and thus these plant-based nanoparticles may be essential in the treatment of various pathologic conditions [35,36]. The morphology of copper nanoparticles synthesized from the extract of Eclipta prostrata leaves as analysed by HRTEM shows spherical and agglomerated particles ranging from 28 to 45 nm [37].…”

Section: Resultsmentioning

confidence: 83%

Controlling Oral Pathogens using Ficus Benghalensis Mediated Silver Nanoparticles

Thirumagal

Jeevitha

et al. 2021

JPRI

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Aim: To find antimicrobial property of Ficus benghalensis mediated silver nanoparticles (AgNPs) Introduction: Nanoparticles have been studied in recent years because of certain properties like physical, chemical, electronic, thermal, magnetic, optical, dielectric and biological. This study was conducted in order to evaluate antimicrobial properties of F. benghalensis mediated AgNPs. Materials and Methods: In this study, extract of F. benghalensis was used to synthesize silver nanoparticles characterized using UV- visible spectrophotometer, Fresh F. benghalensis mediated AgNPs show excellent antimicrobial activity against oral pathogens, Staphylococcus aureus, Streptococcus mutans, Enterococcus faecalis. and Candida albicans. Results: F. benghalensis mediated AgNPs showed antimicrobial activity against gram positive S. aureus with a zone of inhibition of 16.22 ± 0.31 mm at the concentration of 100 μl. The zone of inhibition against S. mutans was 12.14 ± 0.21 mm followed by zone of inhibition against E. faecalis was 12.12 ± 0.2 mm and antimicrobial activity against C. albicans showed a zone of inhibition of 14.02 ± 0.24 mm at the concentration of 100 μl. The zone of inhibition of the nanoparticles was shown to be increased with increase in concentration against all the pathogens and the maximum inhibition was shown against S. aureus. Conclusion: Overall, antimicrobial activity was seen to be increased with increase in concentration. Thus there was potent antimicrobial activity in F. benghalensis mediated AgNPs which could be beneficial when applied in treatment of infectious oral diseases in future.

“…The previous study involves the synthesis of selenium nanoparticles incorporating garlic oil. It can be deduced that garlic oil-mediated selenium nanoparticles have a good cytotoxicity activity at high concentrations [19]. In another study, the green synthesis of selenium nanoparticles was easy and simple.…”

Section: Introductionmentioning

confidence: 96%

Anti-Inflammatory and Cytotoxic Effect of Stevia and Neem Based Herbal Formulation

Selvapriya

Priyadharshini

et al. 2021

JPRI

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Introduction: Nanotechnology is being used in developing countries to treat diseases and prevent health issues. Stevia, conjointly stated as Stevia rebaudiana member of the chrysanthemum family, a subgroup of the Asteraceae family. Azadirachta indica is a native tree to Asian nations. Azadirachta indica could facilitate the fight against plaque buildup and stop periodontal disease. Applied science in drugs needs the employment of nanoparticles to transmit the drug, heat, lightweight, or alternative substances to specific cell sorts like cancer cells. Aim: In this present investigation, Stevia and Neem-based plant extract was prepared and analyzed its anti-inflammatory and cytotoxic effects. Materials and Methods: The plant extract was prepared and an anti-inflammatory and cytotoxic effect was done using a UV-Beckmann spectrometer. The anti-inflammatory activity of nanoparticles prepared using plant extract was investigated by albumin denaturation assay. The results of the test were described as the standard deviation and analyzed using UV-Vis spectroscopy. For the cytotoxic activity, wells are used, wherein the mortality rate of the shrimps was estimated when the plant extract mediated of nanoparticles of different concentrations was added. Results: The nanoparticles synthesized using the stevia and neem extract showed the highest absorbance at a concentration of 50µL (90%) when subjected to inhibition of albumin denaturation assay to check for its anti-inflammatory activity. Brine shrimp lethality was done and the cytotoxicity of these nanoparticles was found to be increasing with increasing concentration of the administered nanoparticles. Conclusion: Anti-inflammatory and cytotoxic activity of Neem and Stevia mediated nanoparticles showed a successful outcome in both the assays.