CYTOTOXICITY OF &lt;i&gt;Phaleria macrocarpa&lt;/i&gt; (Scheff.) Boerl. FRUIT MEAT AND SEED ETHANOL EXTRACT TO MONONUCLEAR PERIFER NORMAL CELL OF HUMAN BODY

Astuti, Endang; Pranowo, Deni; Puspitasari, Santi

doi:10.22146/ijc.21763

Cited by 4 publications

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“…The differences CC50 values between incubation 3 and 5 days were probably due to the compounds of both extracts. The content of alkaloids probably gave a major influence on the cell death when compared to the content of flavonoids in the extracts (Astuti et al, 2006). CC50 values of 70% ethanol extract and water extract were lower than fractionated-70% ethanol extract.…”

Section: Discussionmentioning

confidence: 93%

EFFECT OF VARYING INCUBATION PERIODS ON CYTOTOXICITY AND VIRUCIDAL ACTIVITIES OF Justicia gendarussa Burm.f. LEAF EXTRACT ON HIV-INFECTED MOLT-4 CELLS

Widiyanti¹,

Prajogo²,

Widodo³

2018

AJID

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Backgrounds: Justicia gendarussa Burm.f. has an anti-HIV activity. This study was conducted to evaluate the effects of incubation periods on the cytotoxicity and virucidal activities of the J. gendarussa leaves extract on MOLT-4 cells. Materials and Methods: The cytotoxicity assay was evaluated by using the WST-1 test with incubation periods of 3 days and 5 days. The virucidal activity test was determined by measuring the inhibitory activities on the syncytium formation. Results: The cytotoxicity assay showed the value of CC 50 on MOLT-4 cell culture with the test material of 70% ethanol extract of J. gendarussa leaves as much as 3928.620 µg /mL and 3176.581 µg /mL (incubation day 3 and day 5, respectively); fractionated-70% ethanol extract = 81782.428 µg /mL and 12175.870 µg/mL; and water extract = 16372.689 µg/mL and 2946.117 µg/mL. The test results of the virucidal activities (inhibit ≥ 90% the formation of syncytium) of 70% ethanol extract of J. gendarussa leaves is at a concentration 250 µg/mL, 500 µg/mL and 1000 µg/mL (3-day incubation) and 250 µg/mL (5-day incubation); and fractionated-70% ethanol extract at a concentration 250 µg /mL, 500 µg/mL and 1000 µg/mL (3-day incubation) and 1000 µg/mL (5-day incubation). Conclusion: 70% ethanol extract, fractionated-70% ethanol extract, and water extract of J. gendarussa leaves were relatively nontoxic toward MOLT-4 cells, and fractionated-70% ethanol extract had better potentials in virucidal activities.

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Section: Discussionmentioning

confidence: 93%

EFFECT OF VARYING INCUBATION PERIODS ON CYTOTOXICITY AND VIRUCIDAL ACTIVITIES OF Justicia gendarussa Burm.f. LEAF EXTRACT ON HIV-INFECTED MOLT-4 CELLS

Widiyanti¹,

Prajogo²,

Widodo³

2018

AJID

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“…Various cell signalling activities involved in protein expression of the programmed cells death such as bid, bax, and bcl-2 are likely to be activated when cell lines are exposed to active compounds contained in the extract 25 causing an increase in programmed cell death (apoptosis). 26 Testing by using tetrazolium only measures the formation of formazan (which is related to mitochondria living cell activities) but it is not able to determine the cause of cell death. 27 There was no statistical difference between the two extracts despite having twice the amount of gendarusin A in the extract which indicates that gendarusin A probably does not significantly contribute to the cytotoxicity of the extract.…”

Section: Resultsmentioning

confidence: 99%

Cytotoxicity of Justicia Gendarussa Burm F. Leaf Extracts on Molt-4 Cell

Widiyanti

Prajogo

Hikmawati

2016

IJTID

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Justicia gendarussa Burm f. (Acanthaceae) is known for its activity as a male contraceptive and anti-HIV properties. The present study was designed to evaluate extracts of J. gendarussa for cytotoxicity activity against MOLT-4 cells. The cytotoxic activity of the fractionated-extract and 70% ethanol extracts of J. gendarussa leaves on MOLT-4 cells were evaluated using a WST-1 assay. The treatment cells, control cells without treatment and control media were also tested in duplicate. The absorbance was measured at a wavelength of 450 nm using a microplate absorbance reader (Bio-Rad). The average absorbance measures formazan produced by viable cells that metabolize the WST-1 reagent. Then the data was analyzed with regression analysis Microsoft Excel 2007 program to determine the concentration with 50% cell viability (50% Cytotoxicity Concentration, CC50). The CC50 values of the fractionated-extract and 70% ethanol extract of J. gendarussa leaves were 94 μg/ml and 78 μg/ml, respectively. The cytotoxicity of fractionated-extract and 70% ethanol extract of J. gendarussa leaves were not significantly different (p > 0.05). It can be concluded that the fractionated-extract and 70% ethanol extract of J. gendarussa leaves are not toxic to MOLT-4 cells.

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“…It is apparently due to the compound content of the extract. The presence of alkaloid compounds seems to give a major influence on cell death when it's compared to the flavonoid compounds (Astuti et al, 2006). The influence of alkaloids against cell death could be identified from the results of 70% ethanol extract and water extract of J. gendarussa leaves (Figure 2), where on the sixth day of incubation almost of all concentrations causes the MT-4 cell viability less than 50%.…”

Section: Discussionmentioning

confidence: 99%

ANTIVIRAL ACTIVITY OF Justicia gendarussa Burm.f. LEAVES AGAINST HIV-INFECTED MT-4 CELLS

Widodo¹,

Widiyanti²,

Prajogo³

2018

AJID

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Backgrounds: Justicia gendarussa Burm.f. has been known to have anti-HIV activity. This study was conducted to evaluate the effect of incubation time on the antiviral activity of the J. gendarussa leaves extract on HIV-infected MT-4 cells in vitro. Molecular docking test was also conducted to determine the interaction of alkaloids and flavonoids on the J. gendarussa leaves against HIV-1 reverse transcriptase receptor. It is expected that this research will provide scientific information on the development of J. gendarussa leaves as an anti-HIV drug. Materials and Methods: In the activity test, the effect of incubation time on the antiviral activity of J. gendarussa leaves on HIV-infected MT-4 cells were evaluated. During the activity test, a parameter of cytolysis effect inhibition on MT-4 cell line was observed after 4 days and 6 days incubation period. The molecular docking test is performed by using Molegro Virtual Docker software to determine the interaction of alkaloid and flavonoid compounds of J. gendarussa leaves with HIV-1 reverse transcriptase receptor. Results: The incubation time influences the CC 50 and EC 50 value. Fractionated-70% ethanol extract of J. gendarussa leaves showed a higher anti-HIV activity with EC 50 = 3.045 x 10-9 μg/mL, SI = 6.309 x 10 12 (4 days of incubation) and EC 50 = 6.066 μg/mL, SI = 58494.845 (6 days of incubation). From molecular docking test, it was found that flavonoid of J. gendarussa leaves could inhibit the activity of HIV reverse transcriptase enzyme. Conclusion: Fractionated-70% ethanol extract of J. gendarussa has potential as an anti-HIV.

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CYTOTOXICITY OF <i>Phaleria macrocarpa</i> (Scheff.) Boerl. FRUIT MEAT AND SEED ETHANOL EXTRACT TO MONONUCLEAR PERIFER NORMAL CELL OF HUMAN BODY

Cited by 4 publications

References 0 publications

EFFECT OF VARYING INCUBATION PERIODS ON CYTOTOXICITY AND VIRUCIDAL ACTIVITIES OF Justicia gendarussa Burm.f. LEAF EXTRACT ON HIV-INFECTED MOLT-4 CELLS

EFFECT OF VARYING INCUBATION PERIODS ON CYTOTOXICITY AND VIRUCIDAL ACTIVITIES OF Justicia gendarussa Burm.f. LEAF EXTRACT ON HIV-INFECTED MOLT-4 CELLS

Cytotoxicity of Justicia Gendarussa Burm F. Leaf Extracts on Molt-4 Cell

ANTIVIRAL ACTIVITY OF Justicia gendarussa Burm.f. LEAVES AGAINST HIV-INFECTED MT-4 CELLS

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