Cytotoxicity of Taxol in Combination with Vincristine and Vinblastine Against A375 Cell Line

Abstract

Background: Annually, various types of cancer cause thousands of deaths globally, and identifying an appropriate therapeutic option for these disorders is of crucial importance. Side effects of anticancer drugs can be reduced through the promising strategy of combination therapy. Objectives: The present paper has investigated the in vitro cytotoxicity of Taxol, carboplatin, vinblastine, and vincristine alone and in combination against human malignant melanoma A375 cells and non-cancerous fibroblast HU2 cells to examine the possible side effects of the drugs. Methods: The cells were subjected to the examined compounds for 48 h, and the MTT test was conducted to evaluate the cytotoxicity. Results: The results indicated that the most significant effect was related to 120 μg/mL vincristine and 7.5 μg/mL Taxol+ vincristine treatments, with the survival amounts of 24 ± 0.6 and 28 ± 0%, respectively. In addition, the best 50% inhibitory effect was found to be related to Taxol + vincristine, vinblastine, and Taxol+ vinblastine treatments at the concentrations of 0.04, 2.2, and 3.4 μg/mL, respectively. Conclusions: According to the findings of in vitro toxicity, the evaluated complexes are not cytotoxic against human fibroblast HU2 cells. Also, the most significant effect on A375 cells was associated with vincristine treatment. No synergistic reaction was recorded among the different combinations of drugs based on the calculated CI values.

Trichoderma and sodium nitroprusside elicitation improves vinblastine and vincristine yield by increasing TIA pathway genes expression in cell suspension of Catharanthus roseus

“…Catharanthus roseus is one of the medicinal and ornamental members of Apocynaceae family and it is a sole source of the vincristine and vinblastine as an anticancer drug (Alhaithloul et al 2019; Birat et al 2022). In cell biology, vinblastine and vincristine bind to tubulin and cause polymerization and results in inhibiting microtubule assembly that leads to the disorder in the mitotic spindle, nally metaphase stopped (Zandi 2021). Moreover, the accumulation of these bene cial alkaloids is very limited, which rarely meet the global demand (Zhang et al 2018).…”