Cytotoxicity of the Aqueous Extract and Organic Fractions from <i>Origanum majorana</i> on Human Breast Cell Line MDA-MB-231 and Human Colon Cell Line HT-29

Makrane, Hanane; Messaoudi, Mouhcine El; Melhaoui, Ahmed; Mzibri, Mohammed El; Benbacer, Laïla; Aziz, Mohammed

doi:10.1155/2018/3297193

Cited by 23 publications

(19 citation statements)

References 21 publications

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“…Moreover, the essential oil extracted from the same plant has been reported to inhibit the proliferation of HT-29 cells [26]. The aqueous extract and certain organic fractions from Origanum marjorana were also reported to inhibit the growth of HT-29 cells [27]. Lastly, the phenolic monoterpenoid carvacrol, which was identified as the major constituent in OOEO (47.99%) (Table 1), also possesses significant antiproliferative properties against colon cancer cells, as it was reported to induce cell cycle arrest and apoptosis in two human colon cancer cell lines, HCT-116 and LoVo [28].…”

Section: Resultsmentioning

confidence: 99%

Extraction, Chemical Composition, and Anticancer Potential of Origanum onites L. Essential Oil

et al. 2019

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Origanum species are plants rich in volatile oils that are mainly used for culinary purposes. In recent years, there has been a growing interest in the biological activities of their essential oils. Origanum onites L. is a plant mainly found in Greece, Turkey, and Sicily, whose oil is rich in carvacrol, a highly bioactive phytochemical. The aim of this study was to analyze the chemical composition of Origanum onites essential oil (OOEO), and investigate its potential anticancer effects in vitro and in vivo. GC/MS analysis identified carvacrol as OOEO’s main constituent. In vitro antiproliferative activity was assayed with the sulforhodamine B (SRB) assay against human cancer cell lines from four tumor types. HT-29, a colorectal cancer cell line, was the most sensitive to the antiproliferative activity of OOEO. Wound-healing assay and Annexin V-PI staining were employed to investigate the antimigratory and the pro-apoptotic potential of OOEO, respectively, against human (HT-29) and murine (CT26) colon cancer cells. Notably, OOEO attenuated migration and induced apoptosis-related morphological changes in both cell lines. Prophylactic oral administration of the oil in a BALB/c experimental mouse model inhibited the growth of syngeneic CT26 colon tumors. As far as we know, this is the first report on the antitumor potential of orally administered OOEO.

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Section: Resultsmentioning

confidence: 99%

Extraction, Chemical Composition, and Anticancer Potential of Origanum onites L. Essential Oil

et al. 2019

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“…Genes participated in fatty acid and cholesterol biosynthesis such as acetyl CoA synthase (ACC), 3‐hydroxy‐3‐methylglutaryl‐coenzyme A reductase (HMGCR), sterol regulatory element‐binding protein (SPREPB1), and fatty acid synthase (FASN) were found to be reduced in transcript profile and protein accumulation resulted in cell growth inhibition. Origanum enhanced the BAX expression and downregulated the BCL2 expression by inducing the mitochondrial mediated apoptosis (Balusamy et al, 2018; Makrane et al, 2018). In concentration and time‐dependent manner, carvacrol in human prostate cancer cells decreased cell viability, induced apoptosis, and disrupted MMP (Khan et al, 2017).…”

Section: Health‐promoting Effects (Clinical Trials In Human Cell Lines)mentioning

confidence: 99%

Phytochemical constituents, biological activities, and health‐promoting effects of the genusOriganum

Sharifi‐Rad

Yılmaz

Antika

et al. 2020

Phytotherapy Research

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Origanum species are mostly distributed around the Mediterranean, Euro‐Siberian, and Iran‐Siberian regions. Since time immemorial, the genus has popularly been used in Southern Europe, as well as on the American continent as a spice now known all over the world under the name “oregano” or “pizza‐spice.” Origanum plants are also employed to prepare bitter tinctures, wines, vermouths, beer, and kvass. The major components of Origanum essential oil are various terpenes, phenols, phenolic acids, and flavonoids with predominant occurrence of carvacrol and thymol (with reasonable amounts of p‐cymen and ‐terpinene) or of terpinene‐4‐ol, linalool, and sabinene hydrate. Many species of Origanum genus are used to treat kidney, digestive, nervous, and respiratory disorders, spasms, sore throat, diabetes, lean menstruation, hypertension, cold, insomnia, toothache, headache, epilepsy, urinary tract infections, etc. Origanum essential oil showed potent bioactivities owing to its major constituents' carvacrol, thymol, and monoterpenes. Several preclinical studies evidenced its pharmacological potential as antiproliferative or anticancer, antidiabetic, antihyperlipidemic, anti‐obesity, renoprotective, antiinflammatory, vasoprotective, cardioprotective, antinociceptive, insecticidal, and hepatoprotective properties. Its nanotechnological applications as a promising pharmaceutical in order to enhance the solubility, physicochemical stability, and the accumulation rate of its essential oils have been investigated. However, Origanum has been reported causing angioedema, perioral dermatitis, allergic reaction, inhibition of platelet aggregation, hypoglycemia, and abortion. Conclusive evidences are still required for its clinical applications against human medical conditions. Toxicity analyses and risk assessment will aid to its safe and efficacious application. In addition, elaborate structure–activity studies are needed to explore the potential use of Origanum‐derived phytochemicals as promising drug candidates.

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“…Plants with high amounts of phytoconstituents such as flavonoids and polyphenols are reported to exhibit antioxidant properties [16]. In the current research work, the total polyphenolic content was quantified using the Folin–Ciocalteau assay [22].…”

Section: Discussionmentioning

confidence: 99%

“…IC 50 value (the inhibition concentration required to reduce 50% of cell proliferation) was determined graphically on the basis of regression analysis performed on WST1 assay viability [16].…”

Section: Methodsmentioning

confidence: 99%

Phytochemistry, Antioxidant Activity, Antiproliferative Effect, and Acute Toxicity Testing of Two Moroccan Aristolochia Species

Bourhia

Laasri

Moussa

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Ethnopharmacological Relevance Aristolochia baetica (A. baetica) and Aristolochia paucinervis (A. paucinervis) have been largely used in Moroccan folk medicine. The objective of the study was to investigate the phytochemical composition, the antioxidant activity, the antiproliferative effect, and the acute toxicity of the methanolic extract of A. baetica and A. paucinervis roots. Materials and Methods Phytochemical composition of the methanolic extract of A. baetica and A. paucinervis roots were studied using qualitative and quantitative methods, the antioxidant activity was evaluated using DPPH assay, the antiproliferative effects against human cancer cell lines (T-24, HT-29, and Hep G-2) was assessed using WST1 assay, and the acute toxicity was carried out orally by gavage of single dose 2000 mg/kg to mice for 14 days. Results The two studied plants have different classes of secondary metabolites. The concentrations of the total polyphenolic content of A. baetica and A. paucinervis root extracts were estimated at 360 ± 20 mg GAE/g and 280 ± 27 mg GAE/g, respectively. The total flavonoids content of A. baetica and A. paucinervis extracts were estimated at 35 ± 8 mg QE/g and 235 ± 7 mg QE/g, respectively. A. baetica and A. paucinervis extracts exhibited promising DPPH activity with IC50 values of 150 ± 8 μg/ml and 160 ± 10 μg/ml, respectively. The extracts exerted also antiproliferative effects on all tested cancer cell lines (T-24, HT-29, and Hep G-2) with IC50 values ranging from 6 ± 1 μg/ml to 380 ± 7 μg/ml. Regarding the results of acute toxicity study, no signs of toxicities nor mortalities were observed on the oral treated mice with 2000 mg/kg of the two investigated exacts. Conclusion The methanolic extracts of A. baetica and A. paucinervis possess several phytochemicals that exhibited promising free radical scavenging activity and antiproliferative effects.

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Cytotoxicity of the Aqueous Extract and Organic Fractions from Origanum majorana on Human Breast Cell Line MDA-MB-231 and Human Colon Cell Line HT-29

Cited by 23 publications

References 21 publications

Extraction, Chemical Composition, and Anticancer Potential of Origanum onites L. Essential Oil

Extraction, Chemical Composition, and Anticancer Potential of Origanum onites L. Essential Oil

Phytochemical constituents, biological activities, and health‐promoting effects of the genusOriganum

Phytochemistry, Antioxidant Activity, Antiproliferative Effect, and Acute Toxicity Testing of Two Moroccan Aristolochia Species

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