Cytotoxicity of the Methanol Extract of Datura innoxia Petals on MCF-7 and HEK-293 Cell Lines

Abstract

Background: Medicinal plants are traditionally used to prevent and treat various diseases, including cancer. Despite the development of advanced methods of treatment, cancer mortality is still increasing every year. Medicinal plants are one of the most important sources of anticancer agents. Objectives: The present study was conducted to evaluate the cytotoxic effects of the methanolic extracts of Datura innoxia petals, native to central and South America and distributed in Africa, Asia, Australia, and Europe, on the breast cancer cell line (MCF-7). Materials and Methods: The petals were collected, cleaned, and powdered. The extraction was conducted using the maceration method and then filtered, centrifuged, freeze-dried, and kept at 4°C. The MCF-7 and HEK-293 cell lines were treated with the methanolic extract of D. innoxia petals at various dilutions. Cell viability was quantitated using the MTT assay after 48 and 72 hours. The methanolic extract of D. innoxia petals showed cytotoxic effects on the MCF-7 cell lines at 48 and 72 hours of incubation but did not affect the non-malignant cell line HEK-293. Conclusions: These findings indicate that the methanolic extracts of D. innoxia petals can act as a possible food supplement with anticancer effects and can be used after complementary tests.

Anti-Inflammatory and Anti-cancer Activities of Leaf Extracts from Three Datura Species Found in Mali

“…D. ferox has presented a moderate antiproliferative activity on both cancer cell lines tested. The cytotoxic activity of Datura on these two cancer cell strains has already been reported in the literature [33,35,36,37]. Indeed, with 50 µg/mL methanoic extract of D. stramonium seeds, Iqbal et al [36] have noted a toxicity rate of 72.52% against the MCF-7 cell line IC50 of 265.7 µg/mL and 272.5 µg/mL were observed against HeLa and MCF-7 respectively with leaf ethanolic extracts of D. innoxia harvested in Turkey [33].…”

Social and Economic Burden of Cancer on 2020-Minireview

“…Charaka made fifty (50) groups of ten herbs both of them adequate for a commonplace physician's demand. Sushruta arranged 760 herbs in 7 dissimilar sets based on some of their prevalent properties [67]. Cancer represents an infection which occurs when change in a group of standard cells within the body lead to unrestrained growth, causing a lump called a tumor; this is true of all cancers except leukemia (cancer of the blood).…”

Withanolide Metabolites Inhibit PI3K/AKT and MAPK Pro-Survival Pathways and Induce Apoptosis in Acute Myeloid Leukemia Cells

“…Datura innoxia and Withania coagulanse are two important medicinal plants belonging to family Solanaceae. Extracts of both plants have been extensively investigated for treating different diseases and their putative anticancer properties are well-documented [15][16][17]. In an effort to identify the molecular causes of their reported antineoplastic properties, several withanolides were isolated from these plants and reported to have potent anti-proliferative activities in cancer cells [18], which may be due to their ability to induce apoptosis as well as the expression of tumor necrosis factor-α (TNF-α) activated nuclear factor-κB (NF-κB) [19].…”