Cytotoxicity of the rhizome of medicinal plants

In vitro antioxidant, cytotoxic, thrombolytic activities and phytochemical evaluation of methanol extract of the A. philippense L. leaves

“…The eggs of the brine shrimp were hatched in artificial seawater (3.8% NaCl solution) for 48 h to mature shrimp called nauplii. The cytotoxicity assay was performed on brine shrimp nauplii using the method of Meyer et al which also concurred with the method used by Hossain S et al [19,20] . The test sample was prepared by dissolving them in DMSO (not more than 50 µL in 5 mL solution) with sea water (3.8% NaCl in water) to attain concentrations of 6.25, 12.5, 25, 50, 100, 200 and 400 µg/mL.…”

“…Adiantum philippense L. (A. philippense) (Family-Adiantaceae) locally known as Goyalelata, is a small fern. Stipes 10-15 cm long, tufted, naked, wiry, polished dark chestnut-brown; fronds [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30] cm long and 7.6 cm broad, simply pinnate, often elongated and rooting at the apex; pinnae subdimidiate, the lower edge nearly in a line or oblique with the petiole, the upper edge rounded.…”

Determination of Antiemetic, Antimicrobial, Anti-Radical and Cytotoxic Activity of Methanolic Extracts of <i>Centella asiatica</i>

“…It is established on the killing ability of a simple zoological organism Artemia salina (brine shrimp eggs) used to determine the cytotoxic activity [30].The brine shrimp eggs collected from pet shops was used as the test organism. Two days (48 hours) were allowed to hatch the shrimp and to be matured as naupliiin artificial seawater (3.8% NaCl solution) [31,32]. Constant oxygen supply was provided throughout the hatching time.…”

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“…The cytotoxicity assay was performed on brine shrimp nauplii (Artemia salina) by following the method described by Meyer et al [15] that also concurred with the method used by Hossain et al [16]. The brine shrimp lethality bioassay was used to determine the cytotoxicity of compounds using simple zoological organism Artemia salina as a convenient monitor for the screening.…”