Cytotoxicity study of 32 MEIC chemicals by colony formation and ATP assays

Wakuri, Shinobu; Izumi, Junkichi; Sasaki, Kiyoshi; Tanaka, Noriho; Ono, Hiroshi

doi:10.1016/0887-2333(93)90057-c

Cited by 20 publications

(9 citation statements)

References 12 publications

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“…The IC 50 s of 25-30 chemicals evaluated by the colony formation assay had close correlation (r = 0.73-0.81) with human acute oral lethal doses (LDs), human acute lethal blood concentrations, and mouse oral LD 50 s of these chemicals. 11,23 This fact shows the correlation between the results and human acute toxicity and the applicability of the colony formation assay as a screening test for the investigation of a chemical's toxicity for humans.…”

Section: Discussionmentioning

confidence: 86%

Generic tendency of metal salt cytotoxicity for six cell lines

Yamamoto

Honma

Tanaka

et al. 1999

J. Biomed. Mater. Res.

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Systematic cytotoxicity evaluation of various metallic elements may contribute to the development of new metallic biomaterials with superior biocompatibility. It is generally reported that the cytotoxicity of a chemical differs with cell lines. However, our previous study revealed a high correlation in the cytotoxicity of 43 metal salts between two murine cell lines. If there is any generic tendency toward metal salt cytotoxicity for many kinds of cells, that information is helpful for the determination of the chemical composition of new metallic biomaterials that are expected to have lower cytotoxicity. In this study, the cytotoxicity of 12 metal salts was evaluated using four cell lines, and the results were compared, including those for two other cell lines obtained in our previous study. A metal salt concentration that reduced cell viability to 50% of that without any metal salt (IC(50)) was used as an index to compare the metal salt cytotoxicity between cell lines. The correlation was statistically proved by the IC(50)s of 12 metal salts among these cell lines (p < 0.01), suggesting the existence of a generic tendency to metal salt cytotoxicity beyond cell lines. The metal salt order of toxicity from the highest was K(2)Cr(2)O(7), AgNO(3), VCl(3), SbCl(3), CuCl(2), CoCl(2), NiCl(2), ZnCl(2), Cr(NO(3))(3), FeCl(3), TiCl(4), and Al(NO(3))(3). The sensitivity for metal salt cytotoxicity differed with cell lines; IMR-32 had the highest sensitivity among the six cell lines.

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Section: Discussionmentioning

confidence: 86%

Generic tendency of metal salt cytotoxicity for six cell lines

Yamamoto

Honma

Tanaka

et al. 1999

J. Biomed. Mater. Res.

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“…Assays using cultured cells are toxicity measuring assays that satisfy the requisites described above. The assays using cultured mammalian cells have already been developed by various researchers to measure the toxicity of chemicals for human health 1–5 …”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity evaluation of synthesized chemicals using suspension-cultured fish cells

Mori

Wakabayashi

2000

Fisheries Sci

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SUMMARY: A suspension‐cultured fish cell line was applied to develop a rapid and convenient cytotoxicity assay to evaluate toxicity of a large number of chemicals for fish. Suspension‐cultured fish cells, CHSE‐sp cells, and monolayer‐cultured fish cells, CHSE‐214 cells were used. Cytotoxicity of 11 synthesized chemicals chosen from various chemical classes was determined by the neutral red assay. A good correlation was obtained between cytotoxicity values of 11 chemicals to the CHSE‐sp cells and to the CHSE‐214 cells. Also, good correlations were observed between in vitro toxicity data obtained from the assay using two types of cultured fish cells and in vivo toxicity data obtained from a database on fathead minnow and rainbow trout. From these results, the method using suspension‐cultured fish cells used in this study was considered applicable for a screening test method prior to in vivo testing.

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“…PGD G1, G2, and G3 ( Fig. 1) were synthesized and characterized according to previous reports [37,38]. In brief, TMP used as a starting material was allylated by allyl chloride, which produced a PGD (G0.5).…”

Section: Synthesis and Characterization Of Pgdsmentioning

confidence: 99%

“…Cytotoxicity of PGDs was assayed by the colony formation method [38]. V79 cells were obtained from JCRB Cell Bank (Japan) and cultured in a CO2 incubator (CO2 concentration: 5%, 37°C) using an Eagle's MEM medium (MEM10 medium) containing vol.% of fetal bovine serum (lot number: 24300133, Moregate).…”

Section: Cytotoxic Assaymentioning

confidence: 99%

Temperature-induced recovery of a bioactive enzyme using polyglycerol dendrimers: correlation between bound water and protein interaction

Ooya

Ogawa

Takeuchi

2018

Journal of Biomaterials Science, Polymer Edition

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Enzyme application has gained importance over the past decade in bioprocess, biomedical, and pharmaceutical fields. We found that polyglycerol dendrimers (PGDs), which are biocompatible molecules, can recover alcohol dehydrogenase (ADH) from aqueous solution under elevated temperature. A low concentration of PGD (5 wt.%) is sufficient for the recovery of high enzymatic activity, although a high concentration (25-75 wt.%) of glycerol is generally required to stabilize ADH. The enzymatic activity of ADH in suspension with PGDs is over 60% but it is only 10% in that with glycerol. The results of osmolarity and spin-lattice relaxation time (T) of water measurements in the presence of PGDs suggest that increased amounts of bound water to PGD molecules trigger aggregation along with the direct interaction with ADH. PGDs therefore represent good potential additives for direct recovery of enzymes from aqueous solutions.

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Cytotoxicity study of 32 MEIC chemicals by colony formation and ATP assays

Cited by 20 publications

References 12 publications

Generic tendency of metal salt cytotoxicity for six cell lines

Generic tendency of metal salt cytotoxicity for six cell lines

Cytotoxicity evaluation of synthesized chemicals using suspension-cultured fish cells

Temperature-induced recovery of a bioactive enzyme using polyglycerol dendrimers: correlation between bound water and protein interaction

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