Cytotoxicity Study of Gold Nanoparticles on the Basal-Like Triple-Negative HCC-1937 Breast Cancer Cell Line

Abstract

The Triple Negative "Basal-like" breast cancer (TNBL) tumours have a high proliferative capacity and develop a resistance phenotype associated with metastases. However, the management of TNBL carcinomas is still not standardized. Among the promising trails, gold nanoparticles could be a relevant tool for the development of a targeted treatment for this breast cancer subtype in monotherapy, associated and/or conjugated with other drugs. In this work, we report the cytotoxicity impact of gold nanoparticles wrapped in Poly-Ethylene Glycol (PEG) on the TNBL HCC-1937 breast cancer cell line. PEG-coated gold nanoparticles (PEG-Au NPs) were synthesized by a two-step method using a reduction process followed by a post-functionalization called PEGylation. PEG-Au NPs were characterized using transmission electron microscopy and X-ray diffraction. The gold content of the samples was determined using atomic absorption spectrometer. The cytotoxicity tests were performed using Sulforhodamine B survival test and resazurin viability test. PEG-Au NPs impact analysis on HCC1937 TNBL cell line showed a clear toxic action of type dose dependent and at long term. These PEGylated gold nanoparticles present a promising tool for the development of tumor-specific radiosensitizing vectors, with or without the association of other treatment strategies.

Agri-Nanotechnology and Tree Nanobionics: Augmentation in Crop Yield, Biosafety, and Biomass Accumulation

“…Through LD 50 , the level of cellular toxicity is determined that at what concentration of NPs cause 50% cell mortality and 50% cell viability. The poisoning potential of NPs can be measured through a variety of assays such as 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) ( Raja et al, 2019 ), terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) apoptosis assay ( Jin et al, 2021 ), Alamar blue assay ( Usman et al, 2020 ), sulforhodamine B (SRB) ( Massard et al, 2018 ), and cell counting kit-8 (CCK-8; Dojindo Molecular Technologies) ( Jin et al, 2021 ) can be used to determine the cellular toxicity (mortality or viability percentage). In vivo application of NPs in agriculture can cause genotoxicity to both terrestrial and aquatic animals which can be measured using sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE) assay ( El-Sappah et al, 2022 ).…”