Cytotoxicity Tests on Cultured Rat Skin Fibroblasts Revealed no Toxicity for Low Concentrations of GdYVO<sub>4</sub>:Eu<sup>3+</sup> Nanoparticles

Prokopіuk, Volodymyr; Onishchenko, Anatolii; Yefimova, Svetlana; Chumachenko, Tetyana; Kavok, Nataliya; Maksimchuk, Pavel; Klochkov, Vladimir K.; Tkachenko, Anton

doi:10.1109/nap51885.2021.9568547

Cited by 4 publications

(1 citation statement)

References 11 publications

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“…A complex evaluation of TiO 2 − x NPs was conducted using rat embryonic broblasts using the previously described set of techniques [40]. The cells were isolated by an enzymatic method using 0.25% trypsin-EDTA (Biowest, France) and were cultivated in the SPL culture asks (Republic of Korea) with 5% CO 2 at 37 ° C in Dulbecco's modi ed Eagle's medium (DMEM, Biowest, France) with addition of 10% fetal bovine serum (BioWhittaker® reagents, Lonza, Belgium) up to the third passage.…”

Section: Fibroblast Culturesmentioning

confidence: 99%

Assessing the Cytotoxicity of TiO2−x Nanoparticles with a Different Ti3+(Ti2+)/Ti4+ Ratio

Prokopіuk

Yefimova

Onishchenko

et al. 2022

Biol Trace Elem Res

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Titanium dioxide (TiO 2 ) nanoparticles are promising biomedical agents characterized by good biocompatibility. In this study, we explored the cytotoxicity of TiO 2-x nanoparticles with a different Ti 3+ (Ti 2+ )/Ti 4+ ratio and analyzed the effeciency of eryptosis indices as a tool in nanotoxicology.Methods. Two types of TiO 2-x nanoparticles (5 nm) with various Ti 3+ (Ti 2+ )/ Ti 4+ ratios in the crystal lattice were synthesized. 1-TiO 2-x nanoparticles contained 54% Ti 4+ , 38% Ti 3+ and 8% Ti 2+ , while the relative amount of Ti 4+ and Ti 3+ in the crystal lattice of 2-TiO 2-x nanoparticles was 63% and 37%, respectively. Cell viability and cell motility induced by TiO 2-x nanoparticles were investigated on primary broblast cultures. Eryptosis modulation by the nanoparticles along with cell death mechanisms was studied on rat erythrocytes.Results. We report that both TiO 2-x nanoparticles don't decrease the viability of broblasts simultaneously stimulating cell migration. Data from in vitro studies on erythrocytes indicate that TiO 2-x nanoparticles trigger eryptosis via ROS-(1-TiO 2-x ) and Ca 2+ -mediated mechanisms (both TiO 2-x nanoparticles) suggesting that evaluation of eryptosis parameters is a more sensitive nanotoxicological approach for TiO 2-x nanoparticles than cultured broblast assays.Conclusion. TiO 2-x nanoparticles are characterized by low toxicity against broblasts, but they induce eryptosis, which is shown to be a promising tool for nanotoxicity screening. The Ti 3+ (Ti 2+ )/ Ti 4+ ratio at least partially determines the cytotoxicity mechanisms for TiO 2-x nanoparticles.

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Section: Fibroblast Culturesmentioning

confidence: 99%

Assessing the Cytotoxicity of TiO2−x Nanoparticles with a Different Ti3+(Ti2+)/Ti4+ Ratio

Prokopіuk

Yefimova

Onishchenko

et al. 2022

Biol Trace Elem Res

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Amphi-PIC J-Aggregate - Protein Complexes: Stability in Blood and Toxicity to Cell Cultures

Sorokin

Prokopіuk

Grankina

et al. 2022

2022 IEEE 12th International Conference Nanomaterials: Applications &Amp; Properties (NAP)

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Assessing the Cytotoxicity of Tio2-x Nanoparticles With a Different Ti3+ (Ti2+)/Ti4+ Ratio

Prokopіuk

Yefimova

Onishchenko

et al. 2022

Preprint

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Titanium dioxide (TiO2) nanoparticles are promising biomedical agents characterized by good biocompatibility. In this study, we explored the cytotoxicity of TiO2-x nanoparticles with a different Ti3+(Ti2+)/Ti4+ ratio and analyzed the effeciency of eryptosis indices as a tool in nanotoxicology. Methods. Two types of TiO2-x nanoparticles (5 nm) with various Ti3+ (Ti2+)/ Ti4+ ratios in the crystal lattice were synthesized. 1-TiO2-x nanoparticles contained 54% Ti4+, 38% Ti3+ and 8% Ti2+, while the relative amount of Ti4+ and Ti3+ in the crystal lattice of 2-TiO2-x nanoparticles was 63% and 37%, respectively. Cell viability and cell motility induced by TiO2-x nanoparticles were investigated on primary fibroblast cultures. Eryptosis modulation by the nanoparticles along with cell death mechanisms was studied on rat erythrocytes.Results. We report that both TiO2-x nanoparticles don’t decrease the viability of fibroblasts simultaneously stimulating cell migration. Data from in vitro studies on erythrocytes indicate that TiO2-x nanoparticles trigger eryptosis via ROS- (1-TiO2-x) and Ca2+-mediated mechanisms (both TiO2-x nanoparticles) suggesting that evaluation of eryptosis parameters is a more sensitive nanotoxicological approach for TiO2-x nanoparticles than cultured fibroblast assays.Conclusion. TiO2-x nanoparticles are characterized by low toxicity against fibroblasts, but they induce eryptosis, which is shown to be a promising tool for nanotoxicity screening. The Ti3+ (Ti2+)/ Ti4+ ratio at least partially determines the cytotoxicity mechanisms for TiO2-x nanoparticles.

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Cytotoxicity Tests on Cultured Rat Skin Fibroblasts Revealed no Toxicity for Low Concentrations of GdYVO₄:Eu³⁺ Nanoparticles

Cited by 4 publications

References 11 publications

Assessing the Cytotoxicity of TiO2−x Nanoparticles with a Different Ti3+(Ti2+)/Ti4+ Ratio

Assessing the Cytotoxicity of TiO2−x Nanoparticles with a Different Ti3+(Ti2+)/Ti4+ Ratio

Amphi-PIC J-Aggregate - Protein Complexes: Stability in Blood and Toxicity to Cell Cultures

Assessing the Cytotoxicity of Tio2-x Nanoparticles With a Different Ti3+ (Ti2+)/Ti4+ Ratio

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Cytotoxicity Tests on Cultured Rat Skin Fibroblasts Revealed no Toxicity for Low Concentrations of GdYVO4:Eu3+ Nanoparticles

Cited by 4 publications

References 11 publications

Assessing the Cytotoxicity of TiO2−x Nanoparticles with a Different Ti3+(Ti2+)/Ti4+ Ratio

Assessing the Cytotoxicity of TiO2−x Nanoparticles with a Different Ti3+(Ti2+)/Ti4+ Ratio

Amphi-PIC J-Aggregate - Protein Complexes: Stability in Blood and Toxicity to Cell Cultures

Assessing the Cytotoxicity of Tio2-x Nanoparticles With a Different Ti3+ (Ti2+)/Ti4+ Ratio

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Cytotoxicity Tests on Cultured Rat Skin Fibroblasts Revealed no Toxicity for Low Concentrations of GdYVO₄:Eu³⁺ Nanoparticles