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Kanchanapoom, Kamnoon; Domyoas, Preamrudee

doi:10.2306/scienceasia1513-1874.1999.25.195

Cited by 28 publications

(7 citation statements)

References 20 publications

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“…4d, e). The accumulation of starch grains in the somatic embryos provides an energy source for the development of embryos in oil palm (Kanchanapoom and Domyoas 1999). The histological sections in the present study also demonstrated formation of adventitious shoots from callus (Fig.…”

Section: Histological Analysissupporting

confidence: 76%

Plant regeneration from axillary bud derived callus in white yam (Dioscorea rotundata)

Rajesh

Tripathi

2016

Plant Cell Tiss Organ Cult

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Dioscorea rotundata, commonly known as white yam, is an important staple food crop widely cultivated in West Africa and provides food security to millions of people. Genetic improvements of this crop using the advanced biotechnology tools have been hampered hitherto by the recalcitrant nature of regeneration through somatic embryogenesis. Therefore, we have developed an efficient and reproducible system for plant regeneration via somatic embryogenesis. Explants of different types (immature leaf, node, stem internode, root segment, petiole, and axillary bud) of D. rotundata accession TDr 2436 were tested for their embryogenic potentials on Murashige and Skoog (MS) medium supplemented with various auxins (2,4-D, NAA, and picloram). Among all explants tested, axillary bud explants cultured on MS medium supplemented with picloram (0.5-12 mg/l) favored the production of calli. Maximum proliferation of calli (526 mg fresh weight/explant) was achieved on MS medium supplemented with picloram (0.5 mg/l), casein hydrolysate (600 mg/l), and proline (1 g/l). Histology analysis confirmed that the embryogenic calli produced on this medium were mixed with non-embryogenic calli. Transfer of calli on MS basal medium supplemented with activated charcoal (1 %) changed the color of calli to purple and promoted the production of somatic embryos (87 embryos/callus) as well as adventitious shoot buds. Furthermore, upon transfer to MS medium supplemented with BAP (0.4 mg/l), the embryos continued their differentiation and maturation and germinated into complete plantlets. The adventitious shoot buds produced multiple shoots on MS medium supplemented with BAP (0.4 mg/l). Well-developed germinated plantlets were acclimatized in the screen house with 90 % survivability. Histology studies confirmed that the regeneration of D. rotundata reported here followed dual regeneration pathways. The embryogenic calli regenerated through development of somatic embryos and germinated into complete plantlets, however non-embryogenic calli regenerated through organogenesis and developed multiple shoots. The developed protocol has potential for somatic hybridization, mass clonal propagation, and genetic transformation applications.

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Section: Histological Analysissupporting

confidence: 76%

Plant regeneration from axillary bud derived callus in white yam (Dioscorea rotundata)

Rajesh

Tripathi

2016

Plant Cell Tiss Organ Cult

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“…Three explants were sampled for each period. The materials sampled for histological studies were fixed in FAA 50 (formol: acetic acid: ethylic alcohol 50%, on 5:5:90 ratio) and conserved in 70% ethylic alcohol (Johansen 1940). The samples were embedded in methacrylate (Historesin-Leica) according to the manufacturer recommendations and sectioned transversally and longitudinally in 6 lm slices with a rotatory microtome.…”

Section: Histological and Histochemical Analysesmentioning

confidence: 99%

“…The samples were embedded in methacrylate (Historesin-Leica) according to the manufacturer recommendations and sectioned transversally and longitudinally in 6 lm slices with a rotatory microtome. The sections were then stained with toluidine blue O (O'Brien et al 1964) for metacromasy and stained with Lugol reagent (Johansen 1940) for starch detection. The slides were mounted in synthetic resin (Permount Ò ).…”

Section: Histological and Histochemical Analysesmentioning

confidence: 99%

Histological study of somatic embryogenesis induction on zygotic embryos of macaw palm (Acrocomia aculeata (Jacq.) Lodd. ex Martius)

Moura

Ventrella

Motoike

et al. 2008

Plant Cell Tiss Organ Cult

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The aim of this paper was to describe the histological events that led to somatic embryogenesis in macaw palm (Acrocomia aculeata (Jacq.) Lodd. ex Martius). Zygotic embryos were inoculated on Y 3 medium containing 9 lM 4-amino-3,5,6-trichloropicolonic acid (picloram). Somatic embryos regenerated from nodular callus on induction medium with activated charcoal under photoperiod or without activated charcoal under dark. Many proembryos originated from the fundamental meristem after 10-20 days of culture. When transferred to medium containing activated charcoal, under photoperiod, calli regenerated into somatic embryos of unicellular origin. These embryos had protoderm, plumule and procambial strands and some of them could germinate. After 30-40 days of culture, meristematic masses grew from procambial cells. The masses generated nodular callus, and after transfer to medium without activated charcoal, under dark, they generated somatic embryos of multicellular origin. Those embryos did not regenerate into plants.

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“…On top of AC and putrescine, some other additives are frequently added to the culture medium. These include hydrolyzed casein (Kanchanapoom and Domyoas, 1999; Jayanthi et al, 2011, 2015; Thuzar et al, 2011; Corrêa et al, 2016; Gomes et al, 2016, 2017), the amino acids glutamine (Scherwinski-Pereira et al, 2010; Guedes et al, 2011; de Carvalho Silva et al, 2014; Gomes et al, 2016, 2017; Wan Nur Syuhada et al, 2016; Monteiro et al, 2017), asparagine and arginine (Jayanthi et al, 2015; Corrêa et al, 2016), ascorbic acid (Teixeira et al, 1995; Monteiro et al, 2017), and polyvinylpyrrolidone (Teixeira et al, 1994, 1995), added at variable concentrations.…”

Section: Oil Palm Somatic Embryogenesismentioning

confidence: 99%

“…While, with the exception of a few protocols (Kanchanapoom and Domyoas, 1999; Thuzar et al, 2011; Wan Nur Syuhada et al, 2016), cultures are kept in dark conditions during the EC induction phase, in around 50% of the protocols cultures are transferred to light conditions for induction and maturation of SEs. Regeneration is always performed in light conditions, with a photoperiod of 16 h d −1 and a photosynthetic photon flux varying between 20 and 60 μmol m −2 s −1 .…”

Section: Oil Palm Somatic Embryogenesismentioning

confidence: 99%

Tissue Culture of Oil Palm: Finding the Balance Between Mass Propagation and Somaclonal Variation

Weckx¹,

Inzé

Maene³

2019

Front. Plant Sci.

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The oil palm ( Elaeis guineensis Jacq.) is typically propagated in vitro by indirect somatic embryogenesis, a process in which somatic cells of an explant of choice are, via an intermediate phase of callus growth, induced to differentiate into somatic embryos. The architecture of the oil palm, lacking axillary shoots, does not allow for vegetative propagation. Therefore, somatic embryogenesis is the only alternative to seed propagation, which is hampered by long germination times and low germination rates, for the production of planting material. The current oil palm somatic embryogenesis procedure is associated with several difficulties, which are described in this review. The limited availability of explants, combined with low somatic embryo initiation and regeneration rates, necessitate the proliferation of embryogenic structures, increasing the risk for somaclonal variants such as the mantled phenotype. Several ways to improve the efficiency of the tissue culture method and to reduce the risk of somaclonal variation are described. These include the use of alternative explants and propagation techniques, the introduction of specific embryo maturation treatments and the detection of the mantled abnormality in an early stage. These methods have not yet been fully explored and provide interesting research field for the future. The development of an efficient oil palm micropropagation protocol is needed to keep up with the increasing demand for palm oil in a sustainable way. Mass production of selected, high-yielding palms by tissue culture could raise yields on existing plantations, reducing the need for further expansion of the cultivated area, which is often associated with negative environmental impacts.

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Untitled

Cited by 28 publications

References 20 publications

Plant regeneration from axillary bud derived callus in white yam (Dioscorea rotundata)

Plant regeneration from axillary bud derived callus in white yam (Dioscorea rotundata)

Histological study of somatic embryogenesis induction on zygotic embryos of macaw palm (Acrocomia aculeata (Jacq.) Lodd. ex Martius)

Tissue Culture of Oil Palm: Finding the Balance Between Mass Propagation and Somaclonal Variation

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