Daily ingestion of tetracycline residue present in pasteurized milk: a public health problem

Fernandes, Sérgio Augusto de Albuquerque; Magnavita, Ana Prudência Assis; Ferrão, Sibelli Passini Barbosa; Gualberto, Simone Andrade; Faleiro, Amanda Santos; Figueiredo, Abdias Jose; Matarazzo, Soraia Vanessa

doi:10.1007/s11356-013-2286-5

Cited by 30 publications

(13 citation statements)

References 25 publications

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“…Widespread tetracycline use has fueled concerns that tetracycline residues and their decay products may persist in milk (levels measured at >2mg/L (34)), wastewater (35), the food supply (36), and agricultural settings (37). Tetracycline use is particularly heavy in livestock rearing (37), with estimates that tetracyclines account for two-thirds of total therapeutic antibiotic use in animals (38).…”

Section: Discussionmentioning

confidence: 99%

The Tetracycline Destructases: A Novel Family of Tetracycline-Inactivating Enzymes

Forsberg¹,

Patel²,

Wencewicz³

et al. 2015

Chemistry & Biology

149

162

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Summary Enzymes capable of inactivating tetracycline are paradoxically rare compared with enzymes that inactivate other natural product antibiotics. We describe a family of flavoenzymes, previously unrecognizable as resistance genes, which are capable of degrading tetracycline antibiotics. From soil functional metagenomic selections, we discovered nine genes that confer high-level tetracycline resistance by enzymatic inactivation. We also demonstrate that a tenth enzyme, an uncharacterized homolog in the human pathogen Legionella longbeachae, similarly inactivates tetracycline. These enzymes catalyze the oxidation of tetracyclines in vitro both by known mechanisms and via previously undescribed activity. Tetracycline inactivation genes were identified in diverse soil types, encompass substantial sequence diversity, and are adjacent to genes implicated in horizontal gene transfer. Because tetracycline inactivation is scarcely observed in hospitals, these enzymes may fill an empty niche in pathogenic organisms, and should therefore be monitored for their dissemination potential into the clinic.

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Section: Discussionmentioning

confidence: 99%

The Tetracycline Destructases: A Novel Family of Tetracycline-Inactivating Enzymes

Forsberg¹,

Patel²,

Wencewicz³

et al. 2015

Chemistry & Biology

149

162

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“…The bulk milk can be contaminated with the residues of drugs or chemical agents during their extensive use. Therefore, it may lead to resistance development of antibiotic resistance in the pathogen [ 5 ] or allergic reactions in humans [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Rhodomyrtus tomentosa Leaf Extract on Staphylococcal Adhesion and Invasion in Bovine Udder Epidermal Tissue Model

et al. 2015

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Bovine mastitis is one of the most important infectious diseases in dairy herds, and staphylococci are the most important etiologic agents of this disease. Antibiotics and chemical agents used in livestock for prevention and cure of the disease can accumulate in milk and give rise to food safety concerns. Rhodomyrtus tomentosa leaf extract was studied as an alternative approach to reduce the bacterial infections. The ethanolic extract of this plant demonstrated antibacterial activity with minimum inhibitory concentration (MIC) values as low as 16–64 μg/mL against staphylococcal isolates. In addition, the extract had an effect on the bacterial cell surface properties by increasing its hydrophobicity in a concentration dependent manner. To further extend the antibacterial efficacy, silver nanoparticles synthesized with the extract, a pure rhodomyrtone, and liposomal encapsulated rhodomyrtone were applied and their inhibitory effects on bacterial adhesion and invasion were determined by ex vivo study in a bovine udder epidermal tissue model. These agents exerted remarkable antibacterial activity against staphylococci and decreased the adhesion of the bacterial cells to the tissues. These results supported that R. tomentosa ethanolic extract could be applied as an alternative agent for bovine udder care in dairy farms.

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“…Nero et al (2007) Macedo e Freitas (2009) analisaram 103 amostras de leite de mistura, ou seja, de várias propriedades rurais, usinas de beneficiamento e mercado varejista, através de dois testes enzimáticos e um de inibição microbiana, revelando uma considerável frequência de resíduos de β-lactâmicos em 11 (10,68%) amostras, procedentes de propriedades rurais da região de Conceição do Araguaia, Tucuruí e Santarém. Fernandes et al (2014) buscaram a detecção de resíduos de antibióticos em amostras de leite pasteurizado comercializadas nas regiões sul e sudoeste da Bahia. À partir do método de triagem Delvotest ® SP-NT foi possível detectar 7,54% (19/252) de amostras positivas e 9,52% (24/252) de amostras suspeitas.…”

Section: Resultsunclassified

Detecção de resíduos de antibióticos em leite cru, pasteurizado e Ultra High Temperature (UHT) comercializados no município de Unaí, Minas Gerais

Caldeira

Sola

2020

RSD

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A qualidade do leite pode ser avaliada por parâmetros físico-químicos, sensoriais, microbiológicos e também pela presença de resíduos químicos, como os antimicrobianos. A principal fonte destas substâncias no leite é consequência do manejo inadequado de medicamentos visando o controle de enfermidades no rebanho, descumprimento no descarte de leite durante o período de carência e falhas na higienização de utensílios e equipamentos. Buscou-se neste estudo avaliar a qualidade do leite cru, pasteurizado e UHT comercializados no município de Unaí-MG sob fiscalização dos serviços de inspeção ou comercializados informalmente, quanto à presença de resíduos de antibióticos. Foram analisadas 56 amostras de leite, sendo 16 de leite cru, 16 de leite pasteurizado integral e 24 amostras de leite UHT (8 amostras de leite integral, 8 de semidesnatado e 8 amostras de leite desnatado) a partir do emprego do kit IDEXX SNAP® duo™ ST Plus. Após a realização dos ensaios analíticos foi possível verificar que as amostras de leite pasteurizado e UHT não continham resíduos dos antibióticos testados, enquanto apenas uma amostra de leite cru apresentou-se positiva para a presença de resíduos de β-lactâmicos. Estes resultados indicam a necessidade do cumprimento do período de carência durante o tratamento de animais de produção bem como a necessidade de controle de resíduos de antimicrobianos nas plataformas de recepção de indústrias de laticínios, além da fiscalização por parte do Serviço de Inspeção Oficial visando a produção de alimentos inócuos.

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Daily ingestion of tetracycline residue present in pasteurized milk: a public health problem

Cited by 30 publications

References 25 publications

The Tetracycline Destructases: A Novel Family of Tetracycline-Inactivating Enzymes

The Tetracycline Destructases: A Novel Family of Tetracycline-Inactivating Enzymes

Effects of Rhodomyrtus tomentosa Leaf Extract on Staphylococcal Adhesion and Invasion in Bovine Udder Epidermal Tissue Model

Detecção de resíduos de antibióticos em leite cru, pasteurizado e Ultra High Temperature (UHT) comercializados no município de Unaí, Minas Gerais

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