Damage to oligodendrocytes in the striatum after MPTP neurotoxicity in mice

Takagi, Sho; Hayakawa, Natsumi; Kimoto, Hiroki; Kato, Harubumi; Araki, Tsutomu

doi:10.1007/s00702-007-0790-9

Cited by 35 publications

(27 citation statements)

References 28 publications

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“…After a 9 min rinse in 0.01 M PBS, the sections were incubated with biotinylated secondary antibody for 60 min and then with avidin-biotin peroxidase complex for 30 min at room temperature. Immunoreactivity was visualized using enzyme substrate kits (Vector Labs., Burlingame, CA, USA), as described previously Takagi et al 2007). Negative control study was performed using nonimmunostained IgG by omission of primary antibody, which showed no notable staining.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…For CNPase immunostaining of the dentate gyrus, area of expression of CNPase-immunopositive profiles of dentate granule cell layer, molecular layer, and hilus in the stained sections was evaluated under a light microscope at a magnification of 4009 without the examiner knowing the experimental protocols. These areas were expressed as the area of the CNPase-immunopositive profiles per 1 mm 2 using the computer-associated image analyzer software, as described previously (Takagi et al 2007). All values were expressed as the means ± SE and statistical significance was evaluated by one-way analysis of variance (ANOVA) followed by Fisher's PLSD multiple comparison test (Stat View version 5.0, SAS Institute Inc., USA).…”

Section: Immunohistochemistrymentioning

confidence: 99%

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Alterations of Glial Cells in the Mouse Hippocampus During Postnatal Development

et al. 2009

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We investigated the postnatal alterations of neurons, astrocyte, oligodendrocyte, and microglia in the mouse hippocampal CA1 sector and dentate gyrus under the same conditions using immunohistochemistry. Neuronal nuclei (NeuN), Glial fibrillary acidic protein (GFAP), 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase), and ionized calcium binding adaptor molecule 1 (Iba 1) immunoreactivity were measured in 1-, 2-, 4-, and 8-week-old mice. Total number of NeuN-positive neurons was unchanged in the mouse hippocampal CA1 sector and dentate gyrus from 1 to 8 weeks of birth. In contrast, a significant increase in the number of GFAP-positive astrocytes was observed only in the hippocampal CA1 sector of 1-week-old mice when compared with 8-week-old animals. Thereafter, total number of GFAP-positive astrocytes was unchanged in the hippocampal CA1 sector and dentate gyrus from 2 to 8 weeks of birth. For microglia, a significant increase in the number of Iba 1-positive microglia was observed in the hippocampal CA1 sector and dentate gyrus of 1-, 2-, and 4-week-old mice as compared with 8-week-old animals. On the other hand, a significant decrease in the area of expression of CNPase-positive fibers was observed in the hippocampal CA1 sector of 1- and 2-week-old mice as compared with 8-week-old animals. In dentate gyrus, a significant decrease in the area of expression of CNPase-positive fibers was found in 1-, 2-, and 4-week-old mice. Furthermore, our double-labeled immunostaining showed that brain-derived neurotrophic factor (BDNF) immunoreactivity was observed in GFAP-positive astrocytes and Iba 1-positive microglia in the hippocampal CA1 sector and dentate gyrus of 1- and 2-week-old mice. These results show that glial cells may play some role in the maintenance and neuronal functions of hippocampal CA1 pyramidal neurons and granule cells of dentate gyrus during postnatal development. Furthermore, our results demonstrate that glial BDNF may play an important role in the maturation of oligodendrocyte in the hippocampal CA1 sector and dentate gyrus during postnatal development. Thus, our findings provide valuable information on the developmental processes.

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Section: Immunohistochemistrymentioning

confidence: 99%

Section: Immunohistochemistrymentioning

confidence: 99%

Alterations of Glial Cells in the Mouse Hippocampus During Postnatal Development

et al. 2009

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“…MPTP selectively damages the dopaminergic nigrostriatal system, resulting in the loss of dopaminergic neurons in the substantia nigra and a reduction of dopamine in the striatum (Kurosaki et al 2004(Kurosaki et al , 2005Takagi et al 2007;Watanabe et al 2008). …”

Section: Introductionmentioning

confidence: 98%

Systemic Administration of Proteasome Inhibitor Protects Against MPTP Neurotoxicity in Mice

et al. 2009

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Dysfunction of the proteasome has been suggested to contribute in the degeneration of nigrostriatal dopaminergic neurons. Here, we investigated to determine whether systematic administration of proteasome inhibitor, carbobenzoxy-L: -gamma-t-butyl-L: -glutamyl-L: -alanyl-L: -leucinal (PSI) protects against MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) neurotoxicity in mice. Three administrations of MPTP at 1-h intervals to mice reduced significantly the concentration of dopamine, DOPAC (3,4-dihydroxyphenylacetic acid) and HVA (homovanillic acid) in the striatum after 5 days. In contrast, PSI (0.3 and 1.0 mg/kg) prevented a significant decrease in dopamine, DOPAC and HVA contents of the striatum 5 days after MPTP treatment. In our Western blot analysis study, PSI at a dose of 1.0 mg/kg prevented a significant decrease in TH (tyrosine hydroxylase) protein and a significant increase in glial fibrillary acidic protein 5 days after MPTP treatment. Furthermore, our immunohistochemical study showed that PSI at a dose of 1.0 mg/kg prevented a significant loss in TH immunopositive neurons in the striatum and substantia nigra 5 days after MPTP treatment. In contrast, PSI caused a significant increase in the number of intense ubiquitin immunopositive cells in the striatum and substantia nigra 5 days after MPTP treatment. These results indicate that proteasome inhibitors can protect against MPTP neurotoxicity in mice. The neuroprotective effect of PSI against dopaminergic cell damage may be mediated by the elevation of ubiquitination. Thus, our findings provide further valuable information for the pathogenesis of Parkinson's disease.

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“…Immunoreactivity was visualized using enzyme substrate kits (Vector Labs. ), as described previously Takagi et al 2007). Negative control study was performed using nonimmuned IgG by omission of primary antibody, which showed no notable staining.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Postnatal Development of Neurons, Interneurons and Glial Cells in the Substantia Nigra of Mice

et al. 2010

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We investigated postnatal alterations of neurons, interneurons and glial cells in the mouse substantia nigra using immunohistochemistry. Tyrosine hydroxylase (TH), neuronal nuclei (NeuN), parvalbumin (PV), neuronal nitric oxide synthase (nNOS), glial fibrillary acidic protein (GFAP), ionized calcium-binding adaptor molecule 1 (Iba 1), CNPase (2',3'-cyclic nucleotide 3'-phosphodiesterase), brain-derived neurotrophic factor (BDNF) and glial cell-line-derived neurotrophic factor (GDNF) immunoreactivity were measured in 1-, 2-, 4- and 8-week-old mice. In the present study, the maturation of NeuN-immunopositive neurons preceded the production of TH in the substantia nigra during postnatal development in mice. Furthermore, the maturation of nNOS-immunopositive interneurons preceded the maturation of PV-immunopositive interneurons in the substantia nigra during postnatal development. Among astrocytes, microglia and oligodendrocytes, in contrast, the development process of oligodendrocytes is delayed in the substantia nigra. Our double-labeled immunohistochemical study suggests that the neurotrophic factors such as BDNF and GDNF secreted by GFAP-positive astrocytes may play some role in maturation of neurons, interneurons and glial cells of the substantia nigra during postnatal development in mice. Thus, our findings provide valuable information on the development processes of the substantia nigra.

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Damage to oligodendrocytes in the striatum after MPTP neurotoxicity in mice

Cited by 35 publications

References 28 publications

Alterations of Glial Cells in the Mouse Hippocampus During Postnatal Development

Alterations of Glial Cells in the Mouse Hippocampus During Postnatal Development

Systemic Administration of Proteasome Inhibitor Protects Against MPTP Neurotoxicity in Mice

Postnatal Development of Neurons, Interneurons and Glial Cells in the Substantia Nigra of Mice

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