Penelitian ini bertujuan untuk membandingkan efektifitas bioinsektisida yang berbahan utama sari daun pepaya, dan cabai rawit pada beberapa dosis yang berbeda untuk mengurangi serangan hama pada jamur tiram putih (Pleurotus ostreatus). Ujicoba dilakukan terhadap jamur tiram yang sedang berbuah dengan 3 formula yaitu: formula 1 (daun pepaya dan air), formula 2 (daun pepaya, cabai rawit, dan air), dan formula 3 (cabai rawit dan air). Karakter yang diamati yaitu buah tanggal, berat panen, diameter buah, dan jumlah hama mati. Hasil penelitian menunjukkan bahwa formula berpengaruh terhadap semua karakter yang diamati. Formula 2 memiliki tingkat keefektifan insektisida tertinggi yaitu mampu mengurangi buah tanggal hingga 84 %, memiliki hasil panen 2 kali lipat dan diameter 1,3 kali lipat dibandingkan hasil panen dari kontrol. Formula 2 memiliki komposisi ekstrak daun pepaya, cabai rawit dan air sebesar 1:1:2. Kata kunci: jamur tiram, hama, bioinsektisida, daun pepaya, cabai rawit ABSTRACT This study purposes to compare the effectiveness of bio-insecticides made from papaya leaf extract, and chilli pepper in several different doses to reduce pest attack on white oyster mushroom (Pleurotus ostreatus). Experiments conducted on oyster mushrooms that are fruitful with 3 formula are formula 1 (papaya and water), Formula 2 (papaya leaves, chili pepper, and water), and the formula 3 (chili pepper and water). Parameters observed were the fallen fruit, harvest weight, fruit diameter, the number of dead pests. The results concluded that the formula has a significant effect on all parameters. Formula 2 has the highest level of effectiveness of insecticides can reduce the fallen fruit to 84 %, have twice yields and 1.3 times bigger diameter than control crop yields. Formula 2 has the composition of papaya leaf extract and chili pepper (1:1:2) .