This study was carried out to reveal the characteristic features of honey produced in the Kolludere Valley, which is located within the borders of the Hizan district of Bitlis province. This area is isolated as there are no seasonal migratory beekeepers. In 2022, a flora study was carried out in the area and 133 plant taxa belonging to 19 families from which bees receive nectar or pollen were identified. 23 of these taxa are endemic. Content analysis of honey samples taken from the study area was carried out. Proline value, which is an important parameter of honey quality, was determined as 809.41 mg kg-1, diastase number 28.9, HMF 2.9 mg kg-1, and sucrose 0.2 g/100g. All other parameters (humidity, acidity, pH, fructose+glucose, fructose/glucose, saccharose, maltose, electrical conductivity) were also met standart according to the values of the Food Codex Honey Communiqué and European Union Standards. In addition, pollen analysis of honey samples was made and the data were compared with the flora. In honey samples, pollen is generally minor or rare, and pollen of a dominant taxon was found in only one sample. For this reason, most of the honey produced in the Kolludere Valley was evaluated as flower honey.