DAPI derivative: A fluorescent DNA dye that Can be covalently attached to biomolecules

Li, Min; Wu, Robert S.; Tsai, Jane

doi:10.1016/j.bmcl.2003.09.038

Cited by 15 publications

(19 citation statements)

References 13 publications

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“…The chromatin condensation and fragmentation can be visualized by fluorescence microscopy after DAPI staining. DAPI passes through the intact cell membrane and binds to the minor groove of A-T-rich sequences of DNA with very high affinity [13]. DAPI staining of the C. albicans cells treated with Ple and Ple (4-25) showed concentrated and split fluorescence compared with that of untreated cells, indicating nuclear condensation and fragmentation (Fig.…”

Section: Dna Fragmentation and Nuclear Condensation By Ple And Analogmentioning

confidence: 94%

“…To investigate the effect of the terminal regions on apoptosis, Ple (4-25) and Ple (7-25) were synthesized by cutting GWG (1-3) and GWGSFF (1)(2)(3)(4)(5)(6) in the N-terminal region, respectively. Ple and Ple (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19) were synthesized by deletion of HYL (23)(24)(25) and ALTHYL (20)(21)(22)(23)(24)(25) in the Cterminal region of Ple ( Table 1). The net charges of Ple and Ple (7-25) are +7, like that of Ple, whereas the net charges of Ple and Ple (1-19) are +6 (Table 1).…”

Section: Effect Of Ple-induced Ros On the Apoptosis And Characterizatmentioning

confidence: 99%

“…In addition, AMPs also inhibit the synthesis of intracellular substances such as DNA, RNA, and proteins [3,21]. Recently, some AMPs, such as coprisin [12], RsAFP2 [1], dermaseptin S3 (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16) [18], and pleurocidin (Ple) [4], have been reported to exert an antifungal mechanism via apoptosis induction.…”

Section: Introductionmentioning

confidence: 99%

“…To investigate the functional region of AMPs or to develop effective AMPs with minimal synthesis cost, partially truncated AMPs have been synthesized. The antimicrobial activity was greatly decreased by removing the N-terminal region of HP (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20), suggesting that the Nterminal region was necessary for the antimicrobial activity [19]. On the other hand, LL-31, a truncated peptide missing six amino acids at the C-terminus of LL-37, exhibited a higher killing effect than LL-37 by disrupting the bacterial membrane [11].…”

Section: Introductionmentioning

confidence: 99%

“…In this study, four truncated analogs [Ple , Ple (7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25), Ple , and Ple (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)] were synthesized and the relationship between Ple-induced apoptosis and the role of each truncated N-or C-terminal regions was investigated.…”

Section: Introductionmentioning

confidence: 99%

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The Influence of the N-Terminal Region of Antimicrobial Peptide Pleurocidin on Fungal Apoptosis

Choi

Lee²

2013

J. Microbiol. Biotechnol.

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In our previous study, the 25-mer antimicrobial peptide pleurocidin (Ple) had been thought to induce apoptosis in Candida albicans. This study demonstrated that reactive oxygen species (ROS) production was a major cause of Ple-induced apoptosis. Four truncated analogs were synthesized to understand the functional roles in the N- and C-terminal regions of Ple on the apoptosis. Ple, Ple (4-25), Ple (1-22), and Ple (1-19) produced ROS, including hydroxyl radicals, on the order of [Ple > Ple (1-22) > Ple (4-25) > Ple (1-19)], whereas Ple (7-25) did not induce any ROS production. The results suggested that the N-terminal deletion affected the ROS-inducing activities much more than that of the C-terminal deletion, and net hydrophobicity [Ple > Ple (1-22) > Ple (4-25) > Ple (1-19) > Ple (7-25)] was related to ROS generation rather than other primary factors like net charge. Hence, we focused on the N-terminal-truncated peptides, Ple (4-25) and Ple (7-25), and examined other apoptotic features, including mitochondrial membrane depolarization, caspase activation, phosphatidylserine externalization, and DNA and nuclear fragmentation. The results also confirmed the disappearance of apoptotic activity of Ple (7-25) by the truncation of the N-terminal region (1-6) and the specific activity patterns between Ple and analogs. In conclusion, the N-terminal region of Ple played an important role in apoptosis.

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Section: Dna Fragmentation and Nuclear Condensation By Ple And Analogmentioning

confidence: 94%

Section: Effect Of Ple-induced Ros On the Apoptosis And Characterizatmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Influence of the N-Terminal Region of Antimicrobial Peptide Pleurocidin on Fungal Apoptosis

Choi

Lee²

2013

J. Microbiol. Biotechnol.

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Fluorescence Spectroscopy

Lee¹

2015

CP Essential Lab Tech

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Fluorescence is an extremely powerful tool in modern biology, physics, and chemistry laboratories. This unit begins with the physics of fluorescence, the biological applications of fluorescence, and the mechanisms behind spectrometers and fluorometers, followed by strategies to choose an appropriate fluorophore according to the instrumentation at hand. The first part of the unit walks the reader through the acquisition of an absorption spectrum and explains how it can be used to measure the degree of labeling on conjugated proteins. The discussion then extends into how to obtain fluorescence emission spectra, which can be utilized as fluorescence indicators for protein detection. © 2015 by John Wiley & Sons, Inc.

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Fluorescent UO₂(II) and ZrO(II) complexes: Synthesis, structural characterization, fluorescence, DNA binding studies and biological applications in cell probing

El‐Aziz

El-Wakiel

Gaber

2019

Applied Organom Chemis

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Highlights• New fluorescent UO 2 (II) and ZrO(II) azo-complexes have been synthesized and characterized.• The complexes were tested as cell staining and imaging under fluorescent microscope.• The binding constant (Kb) with DNA has been calculated using UV-vis absorption titration and fluorescence spectral methods.• New ZrO(II) azo-complexe based on 5-nitro-8-hydroxyquinoline can be effectively used as fluorescent probes in studying cell biology.

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DAPI derivative: A fluorescent DNA dye that Can be covalently attached to biomolecules

Cited by 15 publications

References 13 publications

The Influence of the N-Terminal Region of Antimicrobial Peptide Pleurocidin on Fungal Apoptosis

The Influence of the N-Terminal Region of Antimicrobial Peptide Pleurocidin on Fungal Apoptosis

Fluorescence Spectroscopy

Fluorescent UO₂(II) and ZrO(II) complexes: Synthesis, structural characterization, fluorescence, DNA binding studies and biological applications in cell probing

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DAPI derivative: A fluorescent DNA dye that Can be covalently attached to biomolecules

Cited by 15 publications

References 13 publications

The Influence of the N-Terminal Region of Antimicrobial Peptide Pleurocidin on Fungal Apoptosis

The Influence of the N-Terminal Region of Antimicrobial Peptide Pleurocidin on Fungal Apoptosis

Fluorescence Spectroscopy

Fluorescent UO2(II) and ZrO(II) complexes: Synthesis, structural characterization, fluorescence, DNA binding studies and biological applications in cell probing

Contact Info

Product

Resources

About

Fluorescent UO₂(II) and ZrO(II) complexes: Synthesis, structural characterization, fluorescence, DNA binding studies and biological applications in cell probing