Dark-field/hyperspectral microscopy for detecting nanoscale particles in environmental nanotoxicology research

Fakhrullin, Rawil; Nigamatzyanova, Läysän; Fakhrullina, Gölnur

doi:10.1016/j.scitotenv.2021.145478

Cited by 67 publications

(44 citation statements)

References 118 publications

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“…Occasionally, along with the reflected/scattered light from the sample, the out-of-focus light also reaches the objective, reducing the spatial resolution of the image. Therefore, appropriate instrumental adjustments are required to provide the users with vertical scanning capabilities [40]. These aspects should be considered during the analysis.…”

Section: Discussionmentioning

confidence: 99%

Label-Free Detection of Human Coronaviruses in Infected Cells Using Enhanced Darkfield Hyperspectral Microscopy (EDHM)

Gosavi

Cheatham²,

Sztuba-Solińska

2022

J. Imaging

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Human coronaviruses (HCoV) are causative agents of mild to severe intestinal and respiratory infections in humans. In the last 15 years, we have witnessed the emergence of three zoonotic, highly pathogenic HCoVs. Thus, early and accurate detection of these viral pathogens is essential for preventing transmission and providing timely treatment and monitoring of drug resistance. Herein, we applied enhanced darkfield hyperspectral microscopy (EDHM), a novel non-invasive, label-free diagnostic tool, to rapidly and accurately identify two strains of HCoVs, i.e., OC43 and 229E. The EDHM technology allows collecting the optical image with spectral and spatial details in a single measurement without direct contact between the specimen and the sensor. Thus, it can directly map spectral signatures specific for a given viral strain in a complex biological milieu. Our study demonstrated distinct spectral patterns for HCoV-OC43 and HCoV-229E virions in the solution, serving as distinguishable parameters for their differentiation. Furthermore, spectral signatures obtained for both HCoV strains in the infected cells displayed a considerable peak wavelength shift compared to the uninfected cell, indicating that the EDHM is applicable to detect HCoV infection in mammalian cells.

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Section: Discussionmentioning

confidence: 99%

Label-Free Detection of Human Coronaviruses in Infected Cells Using Enhanced Darkfield Hyperspectral Microscopy (EDHM)

Gosavi

Cheatham²,

Sztuba-Solińska

2022

J. Imaging

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show abstract

“…Occasionally, along with the re ected/scattered light from the sample, the out-of-focus light also reaches the objective, reducing the spatial resolution of the image. Therefore, appropriate instrumental adjustments are required to provide the users with vertical scanning capabilities 53 .…”

Section: Discussionmentioning

confidence: 99%

Label-Free Detection of Human Coronaviruses in Infected Cells Using Enhanced Darkfield Hyperspectral Microscopy (EDHM)

Gosavi

Cheatham²,

Sztuba-Solińska

2021

Preprint

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Human coronaviruses (HCoV) are causative agents of mild to severe intestinal and respiratory infections in humans. In the last 15 years, we have witnessed the emergence of three zoonotic, highly pathogenic HCoVs. Thus, early, and accurate detection of these viral pathogens is not only essential for preventing transmission but also for the timely treatment and monitoring of drug resistance. Herein, we applied enhanced darkfield hyperspectral microscopy (EDHM), a novel non-invasive, label-free diagnostic tool for rapid and accurate identification of two strains of HCoVs, i.e., OC43 and 229E. The EDHM technology allows collecting the optical image with both spectral and spatial details in a single measurement without direct contact between the specimen and the sensor. Thus, it can provide the direct mapping of spectral signatures specific for a given viral strain in a complex biological milieu. Our study demonstrated distinct spectral patterns for HCoV-OC43 and HCoV-229E virions in the solution, which can serve as distinguishable parameters for their differentiation. Furthermore, spectral signatures for both HCoV strains in the infected cells displayed a considerable peak wavelength shift compared to the uninfected cell samples indicating that the EDHM is applicable to detect and differentiate between HCoV infected and uninfected cells.

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“…Enhanced dark-field images and hypercubes of PS particles and exposed cells were obtained using the optical system consisting of Olympus BX51 upright microscope (Olympus, Tokyo, Japan) with a motorised stage module ProScan III (JH Technologies, Fremont, CA, USA); a CytoViva ® enhanced dark-field condenser (CytoViva, Auburn, AL, USA) with a halogen light source (Fiber-Lite DC-950, 150 W; Dolan Jenner Industries Inc., Boxborough, MA, USA); an ImSpector V10E spectrograph (Specim, Oulu, Finland); and a Pco.Pixelfly usb CCD camera (PCO AG, Kelheim, Germany). Dark-field images and image sequences during fine focus adjustments were obtained using Exponent 7 image acquisition software (Dage-MTI, Michigan City, IN, USA) at 0.1–1.0 s and 5.0% of exposure time and gain value, respectively [ 38 , 39 ]. Then the hypercubes of the same field of view were captured in the range between 400 and 1000 nm with 2 nm spectral resolution at 0.25 s exposure and full illumination intensity.…”

Section: Methodsmentioning

confidence: 99%

Nanomechanical Atomic Force Microscopy to Probe Cellular Microplastics Uptake and Distribution

Akhatova

Ishmukhametov

Fakhrullina

et al. 2022

IJMS

Self Cite

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The concerns regarding microplastics and nanoplastics pollution stimulate studies on the uptake and biodistribution of these emerging pollutants in vitro. Atomic force microscopy in nanomechanical PeakForce Tapping mode was used here to visualise the uptake and distribution of polystyrene spherical microplastics in human skin fibroblast. Particles down to 500 nm were imaged in whole fixed cells, the nanomechanical characterization allowed for differentiation between internalized and surface attached plastics. This study opens new avenues in microplastics toxicity research.

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Dark-field/hyperspectral microscopy for detecting nanoscale particles in environmental nanotoxicology research

Cited by 67 publications

References 118 publications

Label-Free Detection of Human Coronaviruses in Infected Cells Using Enhanced Darkfield Hyperspectral Microscopy (EDHM)

Label-Free Detection of Human Coronaviruses in Infected Cells Using Enhanced Darkfield Hyperspectral Microscopy (EDHM)

Label-Free Detection of Human Coronaviruses in Infected Cells Using Enhanced Darkfield Hyperspectral Microscopy (EDHM)

Nanomechanical Atomic Force Microscopy to Probe Cellular Microplastics Uptake and Distribution

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