2020
DOI: 10.1016/j.dib.2019.105053
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Data describing the experimental design and quality control of RNA-Seq of human adipose-derived stem cells undergoing early adipogenesis and osteogenesis

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Cited by 8 publications
(17 citation statements)
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“…Transcriptomic analysis confirmed the upregulation of key transcriptional factors during early adipogenesis. Different analysis demonstrated that KLF15 expression was detected in the first 24 h of induction ( Ambele et al, 2016 ; Marcon et al, 2019 , 2020 ), and remained upregulated after 3 ( Spangenberg et al, 2013 ), 7, 14 and 21 ( Ambele et al, 2016 ) days of adipogenic treatment. An augmentation of LMO3, FOXO1, ZBTB16 ( Ambele et al, 2016 ; Marcon et al, 2019 ) CEBPB and CEBPD ( Marcon et al, 2019 ) mRNA was also detected in the total and in the polysome-associated mRNA fraction ( Marcon et al, 2019 ), suggesting not only an upregulation in terms of mRNA abundance but also in protein synthesis rate.…”
Section: Gene Expression Profile In Adipogenesis Of Mscmentioning
confidence: 99%
See 1 more Smart Citation
“…Transcriptomic analysis confirmed the upregulation of key transcriptional factors during early adipogenesis. Different analysis demonstrated that KLF15 expression was detected in the first 24 h of induction ( Ambele et al, 2016 ; Marcon et al, 2019 , 2020 ), and remained upregulated after 3 ( Spangenberg et al, 2013 ), 7, 14 and 21 ( Ambele et al, 2016 ) days of adipogenic treatment. An augmentation of LMO3, FOXO1, ZBTB16 ( Ambele et al, 2016 ; Marcon et al, 2019 ) CEBPB and CEBPD ( Marcon et al, 2019 ) mRNA was also detected in the total and in the polysome-associated mRNA fraction ( Marcon et al, 2019 ), suggesting not only an upregulation in terms of mRNA abundance but also in protein synthesis rate.…”
Section: Gene Expression Profile In Adipogenesis Of Mscmentioning
confidence: 99%
“…The mRNAs associated with polysomes and the rate of translation of the transcripts may also be regulated, modulating protein synthesis. The use of methodologies as the polysome profiling ( Spangenberg et al, 2013 ; Marcon et al, 2020 ) and the ribosome profiling ( Ingolia et al, 2009 , 2012 ; Ingolia, 2016 ; Marcon et al, 2017 ) are interesting to investigate these aspects of gene expression. The analysis and comparison of total or ribosome free mRNA fraction with the one associated to polysomes or ribosomes may provide important information about the level at which genes are being regulated, identifying mechanisms of translational efficiency ( Marcon et al, 2017 ) and of coordinated/opposite actions of the transcriptional and the post-transcriptional mechanisms ( Marcon et al, 2017 ; Pereira et al, 2019 ).…”
Section: Introductionmentioning
confidence: 99%
“…NEFM, GRIN2A, NPTX1, NRG1, and NGF genes are related to nerve cell development and nerve signal transmission. NEFM is associated with neural maturation ( Urrutia et al, 2019 ; Kawase-Koga et al, 2020 ) and is down-regulated after early adipogenic differentiation ( Urrutia et al, 2019 ; Marcon et al, 2020 ; Sadeghi et al, 2020 ; Nie et al, 2021 ). GRIN2A is found in nerve cells (neurons) in the brain and spinal cord and one component of a subset of NMDA receptors.…”
Section: Discussionmentioning
confidence: 99%
“…hASCs were isolated from adipose tissue obtained from female donors who underwent liposuction surgery, as previously described[ 13 ]. Informed consent was provided by each donor.…”
Section: Methodsmentioning
confidence: 99%
“…Immunophenotypic characterization was based on the parameters established for defining mesenchymal stem cells, as indicated by the International Society for Cellular Therapy[ 14 ]. The protocol used followed that previously established in our laboratory[ 13 ]. CD90, CD105, CD73 and CD140b were considered positive markers, while the negative markers were CD19, CD11b, HLA-DR, CD45, CD34 and CD31.…”
Section: Methodsmentioning
confidence: 99%