Data from in vivo functionalization of diatom mesoporous biosilica with bisphosphonates

Abstract

Diatoms are unicellular photosynthetic microalgae that produce a sophisticated mesoporous biosilica shell called frustule . Easy to achieve and extract, diatom frustules represent a low-cost source of mesoporous biocompatible biosilica. In this paper, the possibility to in vivo functionalize the diatom biosilica with bisphosphonates (BPs) was investigated. In particular, two BPs were tested: the amino-containing sodium alendronate (ALE) and the amino-lacking sodium etidronate (ETI). According to first SEM-EDX analysis, the presence of the amino-moiety in ALE structure allowed a better incorporation of this BP into living diatom biosilica, compared to ETI. Then, diatom growth was deeply investigated in presence of ALE. After extraction of functionalized frustules, ALE-biosilica was further characterized by XPS and microscopy, and ALE release was evaluated by ferrochelation assay. Moreover, the bone regeneration performances of ALE-functionalized frustules were preliminarily investigated on bone osteoblast-like cells, via Comassie staining. Data are related to the research article “ In vivo functionalization of diatom biosilica with sodium alendronate as osteoactive material”.

Polydopamine coating of living diatom microalgae

“…5a) and 3D merge reconstruction (Fig. 5b) performed on bare and PDA diatoms show the presence of a dark layer around the cell after the polymerization process: the chemical decoration occurs not only Inside cells, chloroplasts mottles are still red emissive, compartmentalized and polarized: these data, together with a fine cell morphology, constitute strong evidence of maintained diatoms viability, even if the presence of the PDA coating [32]. 3D comparison analysis allowed us to determine that a PDA capsule (1.17 ± 0.388 μm in thickness) is formed all over the whole transparent shell of Thalassiosira weissflogii after functionalization.…”

“…After Guillard stock additions (Na 2 SiO 3 •9H 2 O, trace metals, and a vitamin mix), the medium was buffered with NaOH (2 N) reaching pH 7.8. Growth was controlled following the standard diatoms parameters (T: 18 °C ± 2, Relative % Humidity: 64%, Light:Dark cycle: 16: 8 h, white light 7000 lx) optimized for obtaining cultures for technological applications [32].…”

Copper‐deposited diatom‐biosilica enhanced osteogenic potential in periodontal ligament stem cells and rat cranium

“…It can load a variety of therapeutic factors to form a variety of multifunctional composite biological materials. [30][31][32][33] Copper(II) ions have been reported to have the ability to promote osteoblast migration and differentiation, collagen deposition, angiogenesis, and accelerated wound healing. [34][35][36][37][38][39] However, the clinical applications of Cubased materials present significant limitations due to the potential biotoxicity of localized acute high concentrations of Cu 2+ ions.…”

“…It is a multistage porous marine biological material. It can load a variety of therapeutic factors to form a variety of multifunctional composite biological materials 30–33 . Copper(II) ions have been reported to have the ability to promote osteoblast migration and differentiation, collagen deposition, angiogenesis, and accelerated wound healing 34–39 .…”

Nanostructured interfaces between photosynthetic bacterial Reaction Center and Silicon electrodes

“…Based on a literature protocol, [35] an activation process of Diatomaceous Earth (DE) was exploited for purifying silica matrix from oxides. This method is commonly used for extracting living diatom biosilica [36][37] for photonics, [38] nanoparticles [39][40] and tissue engineering [41][42] applications. After the treatment, casting of the DE suspension in water:acetone 1:1 v/v (DE 4 mg/mL) on pSi, followed by thermal annealing on heating plate at 350°C for 1 hour, were performed in order to produce 2 types of ntSilica:pSi surfaces (1 or 3 layered of DE on pSi, referred as DE1 and DE3).…”