Data highlighting phenotypic diversity of urine-associated Escherichia coli isolates

Eberly, Allison R.; Beebout, Connor J.; Tong, Ching Man Carmen; Horn, G; Green, Hamilton D.; Fitzgerald, Madison J.; De, Shuvro; Apple, Emily K.; Schrimpe‐Rutledge, Alexandra C.; Codreanu, Simona G.; Sherrod, Stacy D.; McLean, John A.; Clayton, Douglass B.; Stratton, Charles W.; Schmitz, Jonathan E.; Hadjifrangiskou, Maria

doi:10.1016/j.dib.2020.105811

Cited by 12 publications

(9 citation statements)

References 5 publications

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“…To evaluate if MGE carriage is a distinguishing feature of UPEC, we conducted whole-genome sequencing of 151 urine-isolated E. coli strains that were previously characterized by their biofilm and metabolic traits (see Table S1 in the supplemental material), as well as the clinical scenarios of their source patients ( 52 , 55 ). These 151 strains originated from individuals with diverse clinical presentations, including ASB (63/151, 41.7%), cystitis (77/151, 50.9%), pyelonephritis (6/151, 3.9%), bacteremia (3/151, 1.9%), and one unclear case (1/151, 0.66%) ( Fig.…”

Section: Resultsmentioning

confidence: 99%

The Role of Mobile Genetic Elements in Virulence Factor Carriage from Symptomatic and Asymptomatic Cases of Escherichia coli Bacteriuria

et al. 2023

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Section: Resultsmentioning

confidence: 99%

The Role of Mobile Genetic Elements in Virulence Factor Carriage from Symptomatic and Asymptomatic Cases of Escherichia coli Bacteriuria

et al. 2023

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“…1B ). To exclude the possibility that the higher HOCl resistance of CFT073 is a strain-specific phenomenon, we analyzed the growth behavior of additional UPEC strains during HOCl stress, including the dominant fluoroquinolone-resistant clone EC958 ( 37 ) and two clinical isolates from cystitis patients, VUTI149 and VUTI247 ( 38 ). Notably, all four UPEC strains showed similar LPE responses in the presence of HOCl and were substantially more resistant than K-12 strain MG1655 ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Redox-Mediated Inactivation of the Transcriptional Repressor RcrR is Responsible for Uropathogenic Escherichia coli’s Increased Resistance to Reactive Chlorine Species

Sultana

Crompton

Meurer

et al. 2022

mBio

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How do pathogens deal with antimicrobial oxidants produced by the innate immune system during infection? Uropathogenic Escherichia coli (UPEC), the most common etiological agent of urinary tract infections (UTIs), is particularly exposed to infiltrating neutrophils and, therefore, must counter elevated levels of the antimicrobial oxidant HOCl to establish infection. Our study provides fundamentally new insights into a defense mechanism that enables UPEC to fend off the toxic effects of HOCl stress.

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“…1B). To exclude the possibility that the higher HOCl resistance of CFT073 is a strain-specific phenomenon, we analyzed the growth behavior of additional UPEC strains during HOCl stress including another common UPEC model strain, the cystitis isolate UTI89 (38), as well as the dominant fluoroquinolone-resistant clone EC958 (39) and two clinical isolates from cystitis patients, VUTI149 and VUTI247 (40). Notably, all five UPEC strains showed similar LPE responses in the presence of HOCl and were substantially more resistant than K-12 strain MG1655 (Fig.…”

Section: Resultsmentioning

confidence: 99%

Redox-mediated inactivation of the transcriptional repressor C3600 makes uropathogenicEscherichia coliexquisitely resistant to reactive chlorine species

Sultana¹,

LeDoux²,

Crompton³

et al. 2021

Preprint

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The ability to overcome stressful environments is critical for pathogen survival in the host. One challenge for bacteria is the exposure to reactive chlorine species (RCS), which are generated by innate immune cells as critical part of the oxidative burst. Hypochlorous acid (HOCl) is the most potent antimicrobial RCS and associated with extensive macromolecular damage in the phagocytized pathogen. However, bacteria have evolved defense strategies to alleviate the effects of HOCl-mediated damage. Among these are RCS-sensing transcriptional regulators that control the expression of HOCl-protective genes under non- and HOCl stress. Uropathogenic Escherichia coli (UPEC), the major causative agent of urinary tract infections (UTIs), is particularly exposed to infiltrating neutrophils during pathogenesis, however, their responses to and defenses of HOCl are still completely unexplored. Here, we present evidence that UPEC strains tolerate higher levels of HOCl and are better protected from neutrophil-mediated killing compared to other E. coli. Transcriptomic analysis of HOCl-stressed UPEC revealed the upregulation of an operon consisting of three genes, one of which encodes the transcriptional regulator C3600. We identified C3600 as a HOCl-sensing transcriptional repressor, which, under non-stress conditions, is bound to the operator and represses the expression of its target genes. During HOCl exposure, however, the repressor forms reversible intermolecular disulfide bonds and dissociates from the DNA resulting in the de-repression of the operon. Deletion of one of the target genes renders UPEC significantly more susceptible to HOCl indicating that the HOCl-mediated induction of the regulon plays a major role for UPEC’s HOCl resistance.

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Data highlighting phenotypic diversity of urine-associated Escherichia coli isolates

Cited by 12 publications

References 5 publications

The Role of Mobile Genetic Elements in Virulence Factor Carriage from Symptomatic and Asymptomatic Cases of Escherichia coli Bacteriuria

The Role of Mobile Genetic Elements in Virulence Factor Carriage from Symptomatic and Asymptomatic Cases of Escherichia coli Bacteriuria

Redox-Mediated Inactivation of the Transcriptional Repressor RcrR is Responsible for Uropathogenic Escherichia coli’s Increased Resistance to Reactive Chlorine Species

Redox-mediated inactivation of the transcriptional repressor C3600 makes uropathogenicEscherichia coliexquisitely resistant to reactive chlorine species

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