Data in support of comparative physiology and proteomic analysis of two wheat genotypes contrasting in drought tolerance

Faghani, Elham; Gharechahi, Javad; Komatsu, Setsuko; Mirzaei, Mehdi; Khavari-Nejad, Ramzan Ali; Najafi, Farzaneh; Farsad, Laleh Karimi; Salekdeh, Ghasem Hosseini

doi:10.1016/j.dib.2014.11.001

Cited by 8 publications

(6 citation statements)

References 3 publications

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“…In this study, the abundance of GST (spot 19, 20 and 32) ( Table 2 and Fig. 4) was the highest when NH4 + was the sole nitrogen source in solution, implying that GST may play a key role in the resistance to NH4 + toxicity, as previously reported for plants under drought stress (Faghani et al, 2015), salinity stress (Wei et al, 2010), cold stress (Badowiec and Weidner, 2014) or pathogen attack (Han et al, 2016). This suggests that GST is widely involved in plant responses to biotic and abiotic stresses.…”

Section: Discussionsupporting

confidence: 85%

Physiological Response and Comparative Proteomic Analysis of Tobacco Seedling Roots to NH4+

Tan¹

2017

IJAB

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Plants absorb nitrogen, an essential element for their growth and development, mainly in the form of nitrate or ammonium. These nitrogen species have different effects on crops, little is known about their precise influence at both physiological and proteomic levels. This study investigated these mechanisms, hydroponically grown tobacco seedlings treated with three different ratios of NO3 -to NH4 + (10:0, 5:5 or 0:10) for 15 days. The results showed that exposure to high NH4 + concentration affected root morphology and inhibited root development. Moreover, malondialdehyde content and the activity of glutamine synthetase, superoxide dismutase, peroxidase and glutathione S-transferase increased with the concentration of NH4 + in solution. Two-dimensional protein gel analysis showed that 40 protein were differentially expressed between the three treatments. 36 of these proteins were identified using liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). Gene ontology analysis indicated that these proteins were involved in metabolism, energy production, cell defense or signal transduction or structural constituents related pathways. Our results suggest that tobacco seedling roots are subjected to NH4 + toxicity that triggers oxidative stress. Our results help understanding the responses to different nitrogen species and to NH4 + toxicity in roots.

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Section: Discussionsupporting

confidence: 85%

Physiological Response and Comparative Proteomic Analysis of Tobacco Seedling Roots to NH4+

Tan¹

2017

IJAB

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“…Interestingly, two Arabidopsis GLPs (AT4G14630; GLP9 and AT3G62020; GLP10) showed stronger binding with the methylated calmodulin (CaM) compared to the unmethylated CaM in presence of calcium indicating the probable involvement of GLPs in root development (Banerjee et al 2013). Differential accumulation of three germin-like proteins (Q9SM34, C3UZE6, and Q9SM34) was reported during drought stress among the sensitive and tolerant wheat roots using nano LC-ESI-MS/MS analysis (Faghani et al 2015). Among those three proteins, Q9SM34 showed top hit entry from the list of the identified protein in the corresponding spot.…”

Section: Expression and Biological Functions Of Glps In Root Tissuesmentioning

confidence: 96%

Versatility of germin-like proteins in their sequences, expressions, and functions

Barman

Banerjee

2015

Funct Integr Genomics

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Germin-like proteins (GLPs) are evolutionary conserved ubiquitous plant glycoproteins belonging to the cupin superfamily. A large number of GLP family members have been identified from different higher and lower plant species, and those have been classified into different subfamilies. Although three histidine residues (H) and one glutamate residue (E) in germin box B and C were conserved among all the GLP subfamily members, how the sequences of one subfamily member differ from the other is unclear. Progress in the field of genomics, transcriptomics, and proteomics has made it possible to understand the variation at gene level among different GLP members from diverse genera and also their biological significances. GLPs from different plant species were found to have various enzymatic properties including oxalate oxidase (OxO), superoxide dismutase (SOD), ADP glucose pyrophosphatase/phosphodiesterase (AGPPase), and polyphenol oxidase (PPO) activities. 'Omics' study demonstrated the expression as well as involvement of GLP family members in almost every part of higher plants as well as in lower plants. Additionally, GLPs from different species were reported to be involved in biotic as well as abiotic stresses and also in the growth and development. This review describes the present research status of GLPs from different plant species, their expressions, and functional significances. Sequence variation was detected among GLP subfamily members at the amino acid level, and based on the sequence variation and phylogenetic analyses, two new GLP subfamilies have been proposed in this review.

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“…Recently many reports on changes in plant proteome under abiotic stress in large number of plant species have been published. Successful proteomics study for drought stress were reported in wheat [15]; Brassica napus [16]; maize [17]; barley [18] and wild watermelon [19]. Under drought stress plants expresses different proteins, not specifically related to drought, other than induced due to the damages of cell.…”

Section: Introductionmentioning

confidence: 99%

Drought mediated physiological and molecular changes in muskmelon (Cucumis melo L.)

et al. 2019

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Water deficiency up to a certain level and duration leads to a stress condition called drought. It is a multi-dimensional stress causing alteration in the physiological, morphological, biochemical, and molecular traits in plants resulting in improper plant growth and development. Drought is one of the major abiotic stresses responsible for loss of crops including muskmelon (Cucumis melo. L). Muskmelon genotype SC-15, which exhibits high drought resistance as reported in our earlier reports, was exposed to deficient water condition and studied for alteration in physiological, molecular and proteomic profile changes in the leaves. Drought stress results in reduced net photosynthetic rate (Pn), stomatal conductance (Gs) and transpiration (E) rate. With expanded severity of drought, declination recorded in content of total chlorophyll and carotenoid while enhancement observed in phenol content indicating generation of oxidative stress. In contrary, activities of catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX), and guaiacol (POD) were increased under drought stress. Peptide mass fingerprinting (PMF) showed that drought increased the relative abundance of 38 spots while decreases10 spots of protein. The identified proteins belong to protein synthesis, photosynthesis, nucleotide biosynthesis, stress response, transcription regulation, metabolism, energy and DNA binding. A drought-induced MADS-box transcription factor was identified. The present findings indicate that under drought muskmelon elevates the abundance of defense proteins and suppresses catabolic proteins. The data obtained exhibits possible mechanisms adopted by muskmelon to counter the impacts of drought induced stress.

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Data in support of comparative physiology and proteomic analysis of two wheat genotypes contrasting in drought tolerance

Cited by 8 publications

References 3 publications

Physiological Response and Comparative Proteomic Analysis of Tobacco Seedling Roots to NH4+

Physiological Response and Comparative Proteomic Analysis of Tobacco Seedling Roots to NH4+

Versatility of germin-like proteins in their sequences, expressions, and functions

Drought mediated physiological and molecular changes in muskmelon (Cucumis melo L.)

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